Information on EC 1.3.1.43 - arogenate dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.3.1.43
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RECOMMENDED NAME
GeneOntology No.
arogenate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-arogenate + NAD+ = L-tyrosine + NADH + CO2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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oxidative decarboxylation
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-
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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L-tyrosine biosynthesis III
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Metabolic pathways
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Novobiocin biosynthesis
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Phenylalanine, tyrosine and tryptophan biosynthesis
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tyrosine metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-arogenate:NAD+ oxidoreductase (decarboxylating)
Arogenate dehydrogenases may utilize NAD+ (EC 1.3.1.43), NADP+ (EC 1.3.1.78), or both (EC 1.3.1.79). NAD+-specific enzymes have been reported from some bacteria [2] and plants [3]. Some enzymes also possess the activity of EC 1.3.1.12, prephenate dehydrogenase.
CAS REGISTRY NUMBER
COMMENTARY hide
64295-75-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 27630 and isolated HGH 154
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
JM300
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Manually annotated by BRENDA team
JM300
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-
Manually annotated by BRENDA team
-
-
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Manually annotated by BRENDA team
29108
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Manually annotated by BRENDA team
29108
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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a maize opaque endosperm mutant (mto140), which shows retarded vegetative growth, is described. The opaque phenotype co-segregates with a mutator transposon insertion in an arogenate dehydrogenase gene (zmAroDH-1). Mto140/arodh-1 seeds show a general reduction in zein storage protein accumulation and an elevated lysine phenotype typical of other opaque endosperm mutants; a mutator insertion at an equivalent position in AroDH-3, the most closely related family member to AroDH-1, is associated with opaque endosperm and stunted vegetative growth phenotypes
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-arogenate + NAD(P)+
L-tyrosine + NAD(P)H + CO2
show the reaction diagram
L-arogenate + NAD+
L-tyrosine + NADH + CO2
show the reaction diagram
prephenate + NAD(P)+
?
show the reaction diagram
prephenate + NAD+
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-arogenate + NAD+
L-tyrosine + NADH + CO2
show the reaction diagram
prephenate + NAD+
?
show the reaction diagram
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3,4-dihydroxyphenylalanine
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slight inhibition
4-amino-L-phenylalanine
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4-hydroxybenzoate
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4-hydroxyphenylacetate
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4-hydroxyphenylpyruvate
D-tyrosine
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DL-4-hydroxyphenyllactate
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L-phenylalanine
L-tyrosine
m-Fluoro-DL-tyrosine
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phenylpyruvate
prephenate
additional information
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no inhibition with phenyllactate and benzoate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-tyrosine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09 - 0.91
L-arogenate
0.01 - 0.41
NAD+
0.07 - 0.25
prephenate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.23
4-hydroxylphenylpyruvate
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0.016 - 0.26
L-tyrosine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0017
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ATCC 27360
0.0058
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HGH 154
3.1
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purified enzyme, substrate prephenate
8.825
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purified enzyme, substrate L-arogenate
18.8
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purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Synechocystis sp. (strain PCC 6803 / Kazusa)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61700
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gel filtration
66000
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dimer, gel filtration
66300
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gel filtration
68000
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gel filtration
69000
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gel electrophoresis after cross-linking with dimethylsuberimidate
150000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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? * 32000, sequence determination and SDS-PAGE
hexamer
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6 * 25500, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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7 days, 90% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in absence of glycerol
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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tyrAc gene, sequence analysis, overexpression of His-tagged protein in Escherichia coli
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tyrc gene, sequence analysis, expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Show AA Sequence (342 entries)
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