Information on EC 1.3.1.38 - trans-2-enoyl-CoA reductase (NADPH)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.3.1.38
-
RECOMMENDED NAME
GeneOntology No.
trans-2-enoyl-CoA reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenation
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of unsaturated fatty acids
-
-
Fatty acid elongation
-
-
Metabolic pathways
-
-
phytol degradation
-
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:NADP+ trans-2-oxidoreductase
Not identical with EC 1.3.1.37 cis-2-enoyl-CoA reductase (NADPH) [cf. EC 1.3.1.8 acyl-CoA dehydrogenase (NADP+)].
CAS REGISTRY NUMBER
COMMENTARY hide
77649-64-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
guinea pig
Uniprot
Manually annotated by BRENDA team
K12
-
-
Manually annotated by BRENDA team
no information in the literature concerning reduction of cis-2-enoyl-CoA substrates, thus classification of rat enzyme according to EC 1.3.1.8 or EC 1.3.1.38 not yet possible
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
-
up-regulated expression upon Erwinia chrysanthemi infection
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-dodecenoyl-acyl carrier protein + NADH
dodecanoyl-acyl carrier protein + NAD+
show the reaction diagram
-
-
-
-
?
2-dodecenoyl-CoA + NADH
dodecanoyl-CoA + NAD+
show the reaction diagram
-
-
-
-
?
2-trans-hexenoyl-CoA + NADPH
?
show the reaction diagram
-
-
-
?
acyl-CoA + NADP+
trans-2,3-dehydroacyl-CoA + NADPH
show the reaction diagram
-
-
-
-
?
crotonyl-CoA + NADPH
butanoyl-CoA + NADP+
show the reaction diagram
nervonyl-CoA + NADPH
tetracosanoyl-CoA + NADP+
show the reaction diagram
-
-
1% of the activity with trans-phytenoyl-CoA
-
ir
octa-2-enoyl-CoA + NADPH
octanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
ir
trans-2,3-dehydrostearoyl-CoA + NADPH
stearoyl-CoA + NADP+
show the reaction diagram
trans-2-decenoyl-CoA + NADPH
decanoyl-CoA + NADP+
show the reaction diagram
trans-2-hexenoyl-CoA + NADPH
hexanoyl-CoA + NADP+
show the reaction diagram
trans-2-hexenoyl-CoA + NADPH + H+
hexanoyl-CoA + NADP+
show the reaction diagram
trans-2-octenoyl-CoA + NADPH
octanoyl-CoA + NADP+
show the reaction diagram
trans-2-octenoyl-CoA + NADPH + H+
octanoyl-CoA + NADP+
show the reaction diagram
trans-phytenoyl-CoA + NADPH + H+
phytanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
ir
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
crotonyl-CoA + NADPH
butanoyl-CoA + NADP+
show the reaction diagram
-
i.e. trans-2-butenoyl-CoA
-
?
trans-2-hexenoyl-CoA + NADPH + H+
hexanoyl-CoA + NADP+
show the reaction diagram
trans-2-octenoyl-CoA + NADPH + H+
octanoyl-CoA + NADP+
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
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i.e. DTNB, inhibition can be prevented by preincubation with NADPH, thus a SH-group is involved in cofactor binding
bovine serum albumin
-
-
-
decanoyl-CoA
-
-
iodoacetic acid
-
-
Lauroyl-CoA
-
-
myristoyl-CoA
-
-
N-ethylmaleimide
Octanoyl-CoA
-
-
p-hydroxymercuribenzoate
-
-
palmitoleoyl-CoA
-
-
palmitoyl-CoA
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Saturated CoA analogs
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-
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Triton X-100
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-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no flavoprotein
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00433 - 0.0054
2-dodecenoyl-CoA
0.0152 - 0.0352
NADH
0.0049 - 0.106
NADPH
0.072
trans-2-crotonyl-CoA
-
-
0.0071 - 0.018
trans-2-decenoyl-CoA
0.0037
trans-2-dodecenoyl-CoA
-
-
0.048
trans-2-hexenoyl-CoA
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-
0.011
trans-2-octenoyl-CoA
-
-
0.018
trans-phytenoyl-CoA
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37C, pH 7.2
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11 - 3.17
2-dodecenoyl-CoA
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.063
decanoyl-CoA
-
-
0.045
Lauroyl-CoA
-
-
0.0092
myristoyl-CoA
-
-
0.198
Octanoyl-CoA
-
-
0.00054
palmitoleoyl-CoA
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-
0.0025
palmitoyl-CoA
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.005
-
substrate crotonyl-CoA
0.05
-
substrate trans-hexenoyl-CoA
0.065
-
substrate trans-hexenoyl-CoA
0.0752
partially purified
0.2
mutant cells over-expressiong MECR-1; mutant cells over-expressiong MECR-1
0.346
purified recombinant enzyme
0.49
-
partially purified enzyme
3.21
cells overexpressing native Etr1p; cells overexpressing native Etr1p
20
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TER mRNA
Manually annotated by BRENDA team
TER mRNA
Manually annotated by BRENDA team
TER mRNA
Manually annotated by BRENDA team
TER mRNA
Manually annotated by BRENDA team
TER mRNA
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
-
-
390749
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
15 min, pH 6.0-8.0
56
-
10 min, pH 6.0, 50% inactivation but reactivation at pH 8.0
60
-
10 min, inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for at least a month, when stored at -20C in PBS containing 50% (v/v) glycerol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, pH 7.0
-
-20C, Tris-HCl buffer, pH 8.0, 10% v/v glycerol
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0C, pH 7.0, 1 mg per ml, 70% of activity remaining after 44 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
associated with peroxisomal acyldihydroxyacetone phosphate reductase; partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli
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cloning of cDNA
cloning of cDNA, expression of TER in HEK-293 cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
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expression as His-tagged protein in Escherichia coli, sequence analysis
expression in Saccharomyces cerevisiae DELTA etr1 cells; expression in Saccharomyces cerevisiae DELTA etr1 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is up-regulated during cotton fibre elongation; expression is up-regulated during cotton fibre elongation
trans-2-enoyl-CoA reductase is one of the most abundant transcripts in the Erwinia chrysanthemi-induced EST library
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G225A
upon expression in Saccharomyces cerevisiae tsc13DELTA mutant significant accumulation of fatty acids C22:0, C20:0, C18:0, C18-OH, and especially C16-OH is detected
G227A
similar to wild-type
G228A
similar to wild-type
G234A
upon expression in Saccharomyces cerevisiae tsc13DELTA mutant significant accumulation of fatty acids C22:0, C20:0, C18:0, C18-OH, and especially C16-OH is detected
I231A
upon expression in Saccharomyces cerevisiae tsc13DELTA mutant significant accumulation of fatty acids C22:0, C20:0, C18:0, C18-OH, and especially C16-OH is detected
P232A
upon expression in Saccharomyces cerevisiae tsc13DELTA mutant significant accumulation of fatty acids C22:0, C20:0, C18:0, C18-OH, and especially C16-OH is detected
Q230A
similar to wild-type
R233A
similar to wild-type
S226A
similar to wild-type
Y229A
similar to wild-type
F149A
-
the mutant is not able to optimally position the cofactor for hydride transfer, which correlates with the 30fold decrease in kcat
K165A
-
normal expression in Escherichia coli
K165Q
-
normal expression in Escherichia coli
Y158F
-
expression in Escherichia coli sub-population BL21(DE3)NH
additional information
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