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Information on EC 1.3.1.21 - 7-dehydrocholesterol reductase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LDU6

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IUBMB Comments
The enzyme is part of the cholesterol biosynthesis pathway.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9LDU6
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
dhcr7, 7-dehydrocholesterol reductase, neverland, daf-36, sterol delta7-reductase, 7-dhc reductase, 3beta-hydroxysterol delta7-reductase, 7-dhcr, 3beta-hydroxysterol-delta7-reductase, 7-dehydrocholesterol delta7-reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sterol DELTA7-reductase
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7-DHC reductase
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Dwarf5 protein
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-
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reductase, 7-dehydrocholesterol
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-
-
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Sterol delta-7-reductase
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-
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sterol DELTA7-reductase
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
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oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
cholesterol:NADP+ DELTA7-oxidoreductase
The enzyme is part of the cholesterol biosynthesis pathway.
CAS REGISTRY NUMBER
COMMENTARY hide
9080-21-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cholesta-5,7-dien-3-beta-ol + NADPH
cholesterol + NADP+
show the reaction diagram
highly regiospecific for DELTA7-bond reduction, but wide substrate specificity
-
?
cholesta-5,7-dien-3beta-ol + NADPH
cholesterol + NADP+
show the reaction diagram
wide substrate spectrum
-
?
ergosta-5,7,22-trien-3beta-ol + NADPH
ergosta-5,22-diene-3beta-ol + NADP+
show the reaction diagram
i.e. ergosterol
-
?
ergosta-5,7-diene-3beta-ol + NADPH
ergosta-5-ene-3beta-ol + NADP+
show the reaction diagram
also ergosta-5,24(28)-, ergosta-5,22,24(28)-eneols and cholesta-5,24- or cholesta-5,22,24-eneols can be formed in vivo in the mutants of Saccharomyces cerevisiae
-
?
ergosta-5,7-diene-3beta-ol + NADPH
ergosta-5-eneol + NADP+
show the reaction diagram
-
-
?
cholesta-5,7-dien-3-beta-ol + NADPH
cholesterol + NADP+
show the reaction diagram
-
-
-
?
cholesta-5,7-dien-3beta-ol + NADPH
cholesterol + NADP+
show the reaction diagram
-
wide substrate spectrum
-
?
ergosta-5,7,22-trien-3beta-ol + NADPH
ergosta-5,22-diene-3beta-ol + NADP+
show the reaction diagram
-
i.e. ergosterol
-
?
ergosta-5,7-diene-3beta-ol + NADPH
ergosta-5-ene-3beta-ol + NADP+
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cholesta-5,7-dien-3-beta-ol + NADPH
cholesterol + NADP+
show the reaction diagram
highly regiospecific for DELTA7-bond reduction, but wide substrate specificity
-
?
ergosta-5,7-diene-3beta-ol + NADPH
ergosta-5-ene-3beta-ol + NADP+
show the reaction diagram
also ergosta-5,24(28)-, ergosta-5,22,24(28)-eneols and cholesta-5,24- or cholesta-5,22,24-eneols can be formed in vivo in the mutants of Saccharomyces cerevisiae
-
?
cholesta-5,7-dien-3-beta-ol + NADPH
cholesterol + NADP+
show the reaction diagram
-
-
-
?
ergosta-5,7-diene-3beta-ol + NADPH
ergosta-5-ene-3beta-ol + NADP+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
no activity with NADH
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ST7R_ARATH
432
9
49591
Swiss-Prot
Secretory Pathway (Reliability: 4)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of cDNA in Saccharomyces cerevisiae and overexpression of recombinant protein Escherichia coli, sequence analysis
gene characterization
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lecain, E.; Chenivesse, X.; Spagnoli, R.; Pompon, D.
Cloning by metabolic interference in yeast and enzymic characterization of Arabidopsis thaliana sterol DELTA7-reductase
J. Biol. Chem.
271
10866-10873
1996
Arabidopsis thaliana (Q9LDU6)
Manually annotated by BRENDA team
Waterham, H.R.; Wanders, R.J.A.
Biochemical and genetic aspects of 7-dehydrocholesterol reductase and Smith-Lemli-Opitz syndrome
Biochim. Biophys. Acta
1529
340-356
2000
Arabidopsis thaliana, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team