Information on EC 1.2.2.1 - formate dehydrogenase (cytochrome)

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The expected taxonomic range for this enzyme is: Gammaproteobacteria

EC NUMBER
COMMENTARY
1.2.2.1
-
RECOMMENDED NAME
GeneOntology No.
formate dehydrogenase (cytochrome)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
formate + 2 ferricytochrome b1 = CO2 + 2 ferrocytochrome b1 + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
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-
-
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reduction
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-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glyoxylate and dicarboxylate metabolism
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SYSTEMATIC NAME
IUBMB Comments
formate:ferricytochrome-b1 oxidoreductase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dehydrogenase, formate (cytochrome)
-
-
-
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FDH
Pseudomonas aeruginosa NCTC 6750
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-
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formate dehydrogenase
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-
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formate dehydrogenase-N
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isozyme
formate dehydrogenase-O
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isozyme
formate:cytochrome b1 oxidoreductase
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-
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formate:phenazine methosulfate oxidoreductase
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-
respiratory formate dehydrogenase
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respiratory formate dehydrogenases are redox enzymes that comprise three subunits: a molybdenum cofactor- and FeS cluster-containing catalytic subunit, an electron-transferring ferredoxin, and a membrane-integral cytochrome b
CAS REGISTRY NUMBER
COMMENTARY
37251-01-7
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
N.C.T.C. 6750
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-
Manually annotated by BRENDA team
Pseudomonas aeruginosa NCTC 6750
N.C.T.C. 6750
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
formate + ferricytochrome b
CO2 + ferrocytochrome b
show the reaction diagram
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-
-
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?
additional information
?
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crude extract of Escherichia coli is investigated for formic dehydrogenase activity. The extract is very active with methylene blue in the presence of formate. Spectroscopic examination shows that the formic dehydrogenase of the extract reacts normally with O2 through cytochrome b
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-
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COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
cytochrome c
-
in its purest state the enzyme contains one molecule of cytochrome co-ordinated by two histidine side chains in the oxidized state
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Iron
-
enzyme contains an iron-sulfur centre
Iron
-
2 mol haeme per mol enzyme, non-haeme iron has not been routinely determined, enzyme contains [3Fe-4S] cluster
Molybdenum
-
enzyme contains molybdenum
Molybdenum
-
1.4 mol per mol enzyme
Se
-
enzyme from cells grown in selenite contains selenium, covalently bound to the largest subunit
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
FdhE binds to the molybdoprotein subunits (FdnG and FdoG) of the respiratory formate dehydrogenase
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
420
-
-
preparation I, 30°C
752
-
-
preparation II, 30°C
760
-
-
preparation III, 30°C
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
cytoplasmic membrane. Transmembranous locations of the 32000 Da subunit and the 110000 Da subunit
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
330000
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-
gel filtration
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
addition of sodium dithionite stabilizes the enzyme
-
663912
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE