Information on EC 1.2.1.84 - alcohol-forming fatty acyl-CoA reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.84
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RECOMMENDED NAME
GeneOntology No.
alcohol-forming fatty acyl-CoA reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a long-chain acyl-CoA + 2 NADPH + 2 H+ = a long-chain alcohol + 2 NADP+ + coenzyme A
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Spodoptera littoralis pheromone biosynthesis
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sporopollenin precursors biosynthesis
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wax esters biosynthesis I
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plasmalogen biosynthesis
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Cutin, suberine and wax biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
long-chain acyl-CoA:NADPH reductase
The enzyme has been characterized from the plant Simmondsia chinensis (jojoba). The alcohol is formed by a four-electron reduction of fatty acyl-CoA. Although the reaction proceeds through an aldehyde intermediate, a free aldehyde is not released. The recombinant enzyme was shown to accept saturated and mono-unsaturated fatty acyl-CoAs of 16 to 22 carbons.
CAS REGISTRY NUMBER
COMMENTARY hide
37350-23-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
degradation of Far1 is accelerated by inhibiting dynamin-, Src kinase-, or flotillin-1-mediated endocytosis without increasing the cellular level of plasmalogens. Far1 is stabilized by sequestering cholesterol with nystatin
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
arachidonoyl-CoA + NADPH
cis-5,8,11,14-eicosatetraenal + NADP+ + CoA
show the reaction diagram
low activity
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?
eicosenoyl-CoA + NADPH + H+
eicosenyl alcohol + NADP+
show the reaction diagram
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about 50% of the activity with stearoyl-CoA
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?
erucoyl-CoA + NADPH + H+
erucyl alcohol + NADP+
show the reaction diagram
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about 30% of the activity with stearoyl-CoA
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?
homo-gamma-linolenoyl-CoA + NADPH
? + NADP+ + CoA
show the reaction diagram
low activity
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?
linoleoyl-CoA + NADPH
cis-9,12-octadecadienal + NADP+ + CoA
show the reaction diagram
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?
oleoyl-CoA + NADPH
cis-9-octadecenal + NADP+ + CoA
show the reaction diagram
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?
palmitoyl-CoA + 2 NADPH + 2 H+
hexadecanol + 2 NADP+ + coenzyme A
show the reaction diagram
palmitoyl-CoA + NADPH
hexadecanal + NADP+ + CoA
show the reaction diagram
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?
stearoyl-CoA + NADPH
octadecanal + NADP+ + CoA
show the reaction diagram
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?
stearoyl-CoA + NADPH + H+
stearyl alcohol + NADP+
show the reaction diagram
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bosine serum albumine
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in vitro, addition of 0.5 mg of bovine serum albumin to the reaction mixture results in a fourfold increase of produced fatty alcohols
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
NADPH
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pH 6.8, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
reaction with palmitoyl-CoA
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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42% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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twofold decrease in enzyme activity compared to 40°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
of stem and leaf
Manually annotated by BRENDA team
; and skin, tissues with highest expression; most abundant in
Manually annotated by BRENDA team
tissue with highest mRNA level
Manually annotated by BRENDA team
highest expression in preputial gland, a specialised sebaceous gland
Manually annotated by BRENDA team
; and eyelid, tissues with highest expression; most abundant in
Manually annotated by BRENDA team
; most abundant in
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
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gel filtration
56000
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1 * 56000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 56000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable during storage, but sensitive to repeated freezing and thawing
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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expression in HEK 293 cells
expression in HEK 293 cells; expression using baculoviral system; expression using baculoviral system
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
abrogation of the asymmetric distribution of plasmalogens in the plasma membrane by reducing the expression of CDC50A encoding a beta-subunit of flippase elevates the expression level of Far1 and plasmalogen synthesis without reducing the total cellular level of plasmalogens
expression is induced by drought and cold stress in an abscisic acid-dependent manner
expression is induced by wounding and salt stress, wounding results in increase in basal levels of C18:0-C22:0 primary alcohols; expression is induced by wounding and salt stress, wounding results in increase in basal levels of C18:0-C22:0 primary alcohols; expression is induced by wounding and salt stress, wounding results in increase in basal levles of C18:0-C22:0 primary alcohols
expression is upregulated in response to drought, cold, and abscisic acid
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