Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.2.1.47 - 4-trimethylammoniobutyraldehyde dehydrogenase and Organism(s) Rattus norvegicus and UniProt Accession Q9JLJ3

for references in articles please use BRENDA:EC1.2.1.47
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
UNIPROT: Q9JLJ3 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
aldh9a1, tmaba-dh, 4-n-trimethylaminobutyraldehyde dehydrogenase, 4-trimethylaminobutyraldehyde dehydrogenase, gamma-trimethylaminobutyraldehyde dehydrogenase, tmabaldehyde-dh ii, carnitine biosynthesis enzyme, aldehyde dehydrogenase 9a1, tmabadh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4-trimethylaminobutyraldehyde dehydrogenase
-
4-trimethylammoniobutyraldehyde dehydrogenase
-
gamma-trimethylaminobutyraldehyde dehydrogenase
-
4-N-trimethylaminobutyraldehyde dehydrogenase
-
-
-
-
4-trimethylaminobutyraldehyde dehydrogenase
-
-
-
-
dehydrogenase, trimethylaminobutyraldehyde
-
-
-
-
TMABA dehydrogenase
-
-
TMABA-DH
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-trimethylammoniobutanal:NAD+ 1-oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
73361-01-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-trimethylaminobutyraldehyde + NAD+
4-trimethylaminobutyrate + NADH + H+
show the reaction diagram
-
-
?
4-trimethylaminobutyraldehyde + NADP+ + H2O
4-trimethylaminobutyrate + NADPH + H+
show the reaction diagram
-
-
-
r
4-trimethylammoniobutanal + NAD+ + H2O
4-trimethylammoniobutanoate + NADH + 2 H+
show the reaction diagram
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
?
betaine aldehyde + NAD+
betaine + NADH
show the reaction diagram
-
-
?
butyraldehyde + NAD+
butyrate + NADH
show the reaction diagram
-
-
?
gamma-aminobutyraldehyde + NAD+
gamma-aminobutyrate + NADH
show the reaction diagram
-
-
?
heptanal + NAD+
heptanoate + NADH
show the reaction diagram
-
-
?
hexadecanal + NAD+
hexadecanoate + NADH
show the reaction diagram
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
?
octanal + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
-
-
?
pentanal + NAD+
pentanoate + NADH
show the reaction diagram
-
-
?
propionaldehyde + NAD+
propionate + NADH
show the reaction diagram
-
-
?
trimethylaminobutyraldehyde + NAD+ + H2O
gamma-butyrobetaine + NADH + 2 H+
show the reaction diagram
enzyme is involved in L-carnitine (3-hydroxy-4-N-trimethylaminobutyrate) biosynthesis
-
-
?
4-trimethylaminobutyraldehyde + NAD+
4-trimethylaminobutanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-trimethylaminobutyraldehyde + NAD+
4-trimethylaminobutyrate + NADH + H+
show the reaction diagram
-
-
?
4-trimethylammoniobutanal + NAD+ + H2O
4-trimethylammoniobutanoate + NADH + 2 H+
show the reaction diagram
-
-
-
?
trimethylaminobutyraldehyde + NAD+ + H2O
gamma-butyrobetaine + NADH + 2 H+
show the reaction diagram
enzyme is involved in L-carnitine (3-hydroxy-4-N-trimethylaminobutyrate) biosynthesis
-
-
?
4-trimethylaminobutyraldehyde + NAD+
4-trimethylaminobutanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
can also be used as oxidant
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
4-Aminobutyraldehyde
-
0.0014
4-trimethylaminobutyraldehyde
-
0.102
acetaldehyde
-
0.123
Betaine aldehyde
-
0.013
Butyraldehyde
-
0.007
Heptanal
-
0.0088
hexadecanal
-
0.009
hexanal
-
0.006
octanal
-
0.01
pentanal
-
0.016
propionaldehyde
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.772
purified enzyme
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
enzyme TMABA-DH colocalizes with myosin light chains in cardiac myocytes
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
medium activity
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
-
highest activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
additional information
alpha-Smooth muscle actin, a marker for endothelial cells, does not colocalize with TMABA-DH signals. The rat enzyme can be detected wit ant-human TMABA-DH antibodies in rat hearts
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AL9A1_RAT
494
0
53653
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
x * 55000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 1 mM dithiothreitol, glycerol, several months
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using column chromatography on SP-Sepharose, Red Sepharose CL-6B and Hydroxylapatite
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
detection of mRNA levels in foetuses, increase during postnatal life, expression is ontogenically regulated
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Vaz, F.M.; Fouchier, S.W.; Ofman, R.; Sommer, M.; Wanders, R.J.A.
Molecular and biochemical characterization of rat gamma-trimethylaminobutyraldehyde dehydrogenase and evidence for the involvement of human aldehyde dehydrogenase 9 in carnitine biosynthesis
J. Biol. Chem.
275
7390-7394
2000
Rattus norvegicus (Q9JLJ3), Rattus norvegicus Wistar (Q9JLJ3)
Manually annotated by BRENDA team
van Vlies, N.; Wanders, R.J.; Vaz, F.M.
Measurement of carnitine biosynthesis enzyme activities by tandem mass spectrometry: differences between the mouse and the rat
Anal. Biochem.
354
132-139
2006
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Garcia-Delgado, M.; Peral, M.J.; Duran, J.M.; Garcia-Miranda, P.; Calonge, M.L.; Ilundain, A.A.
Ontogeny of Na(+)/L-carnitine transporter and of gamma-trimethylaminobutyraldehyde dehydrogenase and gamma-butyrobetaine hydroxylase genes expression in rat kidney
Mech. Ageing Dev.
130
227-233
2009
Rattus norvegicus (Q9JLJ3)
Manually annotated by BRENDA team
Matsunaga, A.; Harita, Y.; Shibagaki, Y.; Shimizu, N.; Shibuya, K.; Ono, H.; Kato, H.; Sekine, T.; Sakamoto, N.; Igarashi, T.; Hattori, S.
Identification of 4-trimethylaminobutyraldehyde dehydrogenase (TMABA-DH) as a candidate serum autoantibody target for Kawasaki disease
PLoS ONE
10
e0128189
2015
Homo sapiens (P49189), Homo sapiens, Rattus norvegicus (Q9JLJ3)
Manually annotated by BRENDA team