Information on EC 1.2.1.32 - aminomuconate-semialdehyde dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
1.2.1.32
-
RECOMMENDED NAME
GeneOntology No.
aminomuconate-semialdehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-aminomuconate 6-semialdehyde + NAD+ + H2O = 2-aminomuconate + NADH + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidation
-
-
oxidation
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
reduction
-
-
reduction
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-amino-3-carboxymuconate semialdehyde degradation to 2-oxopentenoate
-
-
2-amino-3-carboxymuconate semialdehyde degradation to glutaryl-CoA
-
-
2-aminophenol degradation
-
-
2-nitrobenzoate degradation I
-
-
Tryptophan metabolism
-
-
tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
2-aminomuconate-6-semialdehyde:NAD+ 6-oxidoreductase
Also acts on 2-hydroxymuconate semialdehyde.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2-aminomuconate semialdehyde dehydrogenase
-
-
2-aminomuconate semialdehyde dehydrogenase
Burkholderia cepacia J2315
-
-
-
2-aminomuconic 6-semialdehyde dehydrogenase
-
-
-
-
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate dehydrogenase
-
-
-
-
2-hydroxymuconate semialdehyde dehydrogenase
-
-
-
-
2-hydroxymuconic 6-semialdehyde dehydrogenase
-
-
2-hydroxymuconic 6-semialdehyde dehydrogenase
-
-
-
2-hydroxymuconic acid semialdehyde dehydrogenase
-
-
-
-
2-hydroxymuconic semialdehyde dehydrogenase
-
-
-
-
2-hydroxymuconic semialdehyde dehydrogenase
-
-
alpha-aminomuconic epsilon-semialdehyde dehydrogenase
-
-
-
-
alpha-hydroxymuconic epsilon-semialdehyde dehydrogenase
-
-
-
-
AMDH
Burkholderia cepacia J2315
-
-
-
aminomuconate semialdehyde dehydrogenase
-
-
-
-
aminomuconate-semialdehyde dehydrogenase
-
-
aminomuconate-semialdehyde dehydrogenase
-
-
-
aminomuconate-semialdehyde dehydrogenase
-
-
aminomuconate-semialdehyde dehydrogenase
Burkholderia cepacia J2315
-
-
-
dehydrogenase, 2-hydroxymuconate semialdehyde
-
-
-
-
dehydrogenase, aminomuconate semialdehyde
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37250-95-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Azotobacter sp.
-
-
-
Manually annotated by BRENDA team
Bordetella sp. strain 10d; strain 10d
-
-
Manually annotated by BRENDA team
strain 10d
-
-
Manually annotated by BRENDA team
; strain J2315
-
-
Manually annotated by BRENDA team
strain G4, formerly Pseudomonas cepacia
SwissProt
Manually annotated by BRENDA team
Burkholderia cepacia G4
strain G4, formerly Pseudomonas cepacia
SwissProt
Manually annotated by BRENDA team
Burkholderia cepacia J2315
strain J2315
-
-
Manually annotated by BRENDA team
basonym Alcaligenes eutrophus, strain 335
-
-
Manually annotated by BRENDA team
Cycloclasticus oligotrophus RB1
RB1
-
-
Manually annotated by BRENDA team
Escherichia coli MSO22
MSO22
-
-
Manually annotated by BRENDA team
strain JS45 grown on nitrobenzene
-
-
Manually annotated by BRENDA team
Pseudomonas pseudoalcaligenes JS45
strain JS45 grown on nitrobenzene
-
-
Manually annotated by BRENDA team
enzyme inducible by aromatic amines; strain mt-2 (ATCC 33015) carrying the TOL plasmid pWW0
-
-
Manually annotated by BRENDA team
strain mt-2 (ATCC 33015) carrying the TOL plasmid pWW0
-
-
Manually annotated by BRENDA team
strain mt-2 (ATCC 33015) carrying the TOL plasmid pWW0; strain mt-2 (ATCC 33015) grown on toluene or 1,2,4-trimethylbenzene
-
-
Manually annotated by BRENDA team
strain NCIB 10015 grown on phenol or cresols
-
-
Manually annotated by BRENDA team
Pseudomonas putida NCIB 10015
strain NCIB 10015 grown on phenol or cresols
-
-
Manually annotated by BRENDA team
carrying the naphthalene plasmids NAH2 or NAH3
-
-
Manually annotated by BRENDA team
o-cresol adapted cells
-
-
Manually annotated by BRENDA team
phenantrene utilizing strain DJ77
-
-
Manually annotated by BRENDA team
strain CF600
-
-
Manually annotated by BRENDA team
strain CF600
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
show the reaction diagram
Burkholderia cepacia J2315
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + 2 H+
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia J2315
-
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
metabolism of benzene ring of tryptophan in mammalian tissues
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
Pseudomonas pseudoalcaligenes JS45
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
Pseudomonas pseudoalcaligenes JS45
-
-
-
?
2-aminomuconic acid semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-fluorobenzaldehyde + NAD+ + H2O
2-fluorobenzoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
2-hydroxy-5-methylmuconate + NADH
show the reaction diagram
-
synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
-
-
?
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
2-hydroxy-5-methylmuconate + NADH
show the reaction diagram
-
synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
-
-
?
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
2-hydroxy-5-methylmuconate + NADH
show the reaction diagram
Cycloclasticus oligotrophus, Cycloclasticus oligotrophus RB1
-
synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
-
-
?
2-hydroxy-5-methyl-muconic-6-semialdehyde + NAD+
2-hydroxy-5-methylmuconate + NADH
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia G4
Q83XU8
most effective substrate
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
Cycloclasticus oligotrophus, Cycloclasticus oligotrophus RB1
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
show the reaction diagram
Q83XU8
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
show the reaction diagram
Q83XU8
involved in meta cleavage pathway of aromatic hydrocarbons
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
show the reaction diagram
Burkholderia cepacia G4
Q83XU8
involved in meta cleavage pathway of aromatic hydrocarbons
-
-
?
2-hydroxymuconic 6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
substrate for spectrophotometric activity assay, relative activity 100%
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
Cupriavidus necator, Cupriavidus necator 345, Cupriavidus necator RA1012
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
Pseudomonas putida NCIB 10015
-
dissimilatory meta-cleavage of catechols
-
?
3-chlorobenzaldehyde + NAD+ + H2O
3-chlorobenzoate + NADH
show the reaction diagram
-
low activity
-
-
?
3-fluorobenzaldehyde + NAD+ + H2O
3-fluorobenzoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH
show the reaction diagram
-
low activity
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
3-methylbenzoate + NADH
show the reaction diagram
-
low activity
-
-
?
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH
show the reaction diagram
-
low activity
-
-
?
4-carboxybenzaldehyde + NAD+ + H2O
4-carboxybenzoate + NADH
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
4-chlorobenzoate + NADH
show the reaction diagram
-
low activity
-
-
?
4-fluorobenzaldehyde + NAD+ + H2O
4-fluorobenzoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
4-methoxybenzoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
4-methylbenzoate + NADH
show the reaction diagram
-
low activity
-
-
?
4-nitrobenzaldehyde + NAD+ + H2O
4-nitrobenzoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
5-chloro-2-hydroxymuconic-6-semialdehyde + NAD+
5-chloro-2-hydroxymuconate + NADH
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia G4
Q83XU8
poor substrate
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
relative activity 113%
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
relative activity 113%
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
Pseudomonas pseudoalcaligenes JS45
-
low activity
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
-
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
relative activity 25%
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
Pseudomonas pseudoalcaligenes JS45
-
low activity
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
relative activity 138%
-
-
?
crotonaldehyde + NAD+ + H2O
crotonate + NADH
show the reaction diagram
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
-
low activity
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
-
low activity
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
-
relative activity 16,5%
-
-
?
n-butanal + NAD+ + H2O
n-butanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
n-butanal + NAD+ + H2O
n-butanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
n-butanal + NAD+ + H2O
n-butanoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
propanal + NAD+ + H2O
n-propanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
n-propanoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
relative activity 107%
-
-
?
pyridine 4-carboxyaldehyde + NAD+ + H2O
pyridine 4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
hexaldehyde + NAD+ + H2O
n-hexanoic acid + NADH
show the reaction diagram
-
low activity
-
-
?
additional information
?
-
-
no reaction with 2-methylbenzaldehyde
-
-
-
additional information
?
-
-
no reaction with 2-chlorobenzaldehyde
-
-
-
additional information
?
-
-
no reaction with 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde
-
-
-
additional information
?
-
Burkholderia cepacia, Burkholderia cepacia G4
Q83XU8
no activity with 2-hydroxy-6-oxo-hepta-2,4-dienoic acid and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-aminomuconate 6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH + H+
show the reaction diagram
Burkholderia cepacia J2315
-
-
-
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
metabolism of benzene ring of tryptophan in mammalian tissues
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
2-aminomuconate + NADH
show the reaction diagram
Pseudomonas pseudoalcaligenes JS45
-
catabolism of nitrobenzene as sole source of carbon, nitrogen and energy
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH
show the reaction diagram
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
4-oxalocrotonate + NADH + H+
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia G4
Q83XU8
involved in meta cleavage pathway of aromatic hydrocarbons
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
-
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
Cupriavidus necator, Cupriavidus necator 345, Cupriavidus necator RA1012
-
dissimilatory meta-cleavage of catechols
-
?
2-hydroxymuconic acid semialdehyde + NAD+ + H2O
2-hydroxymuconate + NADH + H+
show the reaction diagram
Pseudomonas putida NCIB 10015
-
dissimilatory meta-cleavage of catechols
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
specific for, not replaceable by NADP+
NAD+
Q83XU8
no activity in the absence of NAD+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-Chlorobenzaldehyde
-
competitive
2-Methoxybenzaldehyde
-
competitive
Co2+
-
0.2 mM, almost complete inhibition
Co2+
-
-
Cu2+
-
0.2 mM, almost complete inhibition
Cu2+
-
-
CuSO4
-
0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibtion at 1 mM
Hg2+
-
0.2 mM, almost complete inhibition
Hg2+
-
-
HgCl2
-
0% remaining activity at an inhibitor concentration of 1 mM; 100% inhibtion at 1 mM
iodoacetamide
-
equimolar amount reduces benzaldehyde oxidation activity by 44%
iodoacetic acid
-
31% inhibtion at 1 mM; 69% remaining activity at an inhibitor concentration of 1 mM
N-ethylmaleimide
-
equimolar amount inactivates benzaldehyde oxidation completely, 5 mM 2-mercaptoethanol restores activity
N-ethylmaleimide
-
18% remaining activity at an inhibitor concentration of 1 mM
N-Methylmaleimide
-
82% inhibtion at 1 mM
Ni2+
-
0.2 mM, almost complete inhibition
Ni2+
-
-
p-chloromercuribenzoate
-
equimolar amount inactivates benzaldehyde oxidation completely, 5 mM 2-mercaptoethanol restores activity
p-chloromercuribenzoate
-
100% inhibition at 20 mM, 52% inhibition at 0.01 mM, 1 mM cysteine reverses inhibition completely
p-chloromercuribenzoate
-
-
p-chloromercuribenzoic acid
-
41% remaining activity at an inhibitor concentration of 1 mM; 59% inhibtion at 1 mM
ZnSO4
-
26% remaining activity at an inhibitor concentration of 1 mM; 74% inhibtion at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
HMSD in crude extracts prepared from pDTG912/B shows higher activity in potassium phosphate buffer than in Tris-HCl buffer (50 mM)
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2
2-Fluorobenzaldehyde
-
-
0.0093
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
synonym 5-methyl-2-hydroxymuconic-6-semialdehyde
0.002
2-hydroxy-5-methyl-muconic-6-semialdehyde
Q83XU8
pH 8.5, 25C
0.011
2-hydroxymuconate-6-semialdehyde
Q83XU8
pH 8.5, 25C
0.016
2-hydroxymuconate-6-semialdehyde
-
-
0.017
2-hydroxymuconate-6-semialdehyde
-
-
0.026
2-hydroxymuconate-6-semialdehyde
-
-
0.48
2-nitrobenzaldehyde
-
-
1.3
3-Chlorobenzaldehyde
-
-
0.17
3-Fluorobenzaldehyde
-
-
0.94
3-Methoxybenzaldehyde
-
-
0.63
3-methylbenzaldehyde
-
-
0.81
3-Nitrobenzaldehyde
-
-
0.7
4-Chlorobenzaldehyde
-
-
0.31
4-Fluorobenzaldehyde
-
-
1.1
4-Methoxybenzaldehyde
-
-
0.53
4-methylbenzaldehyde
-
-
0.31
4-Nitrobenzaldehyde
-
-
0.083
5-chloro-2-hydroxymuconic-6-semialdehyde
Q83XU8
pH 8.5, 25C
0.46
benzaldehyde
-
-
0.019
NAD+
-
-
0.046
NAD+
Q83XU8
pH 8.5, 25C, with 2-hydroxy-5-methyl-muconic-6-semialdehyde as substrate
0.294
NAD+
Q83XU8
pH 8.5, 25C, with 2-hydroxymuconate-6-semialdehyde as substrate
0.33
NAD+
-
-
1.316
NAD+
Q83XU8
pH 8.5, 25C, with 5-chloro-2-hydroxymuconic-6-semialdehyde as substrate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
99300
2-hydroxy-5-methyl-muconic-6-semialdehyde
Q83XU8
pH 8.5, 25C
21900
2-hydroxymuconate-6-semialdehyde
Q83XU8
pH 8.5, 25C
2300
5-chloro-2-hydroxymuconic-6-semialdehyde
Q83XU8
pH 8.5, 25C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.13
2-Chlorobenzaldehyde
-
-
0.06
2-Methoxybenzaldehyde
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0037
-
after streptomycin sulfate treatment
0.0041
-
cell extract
0.0092
-
after ammonium sulfate precipitation
0.046
-
after DE52 chromatography
0.071
-
after DEAE-Toyopearl chromatography
0.075
-
after DEAE-Cellulofine chromatography
0.0765
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.3
-
2-aminomuconic acid oxidation
7.5 - 8
-
-
9.6
-
benzaldehyde oxidation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 9
-
more than 50% of maximal activity between pH 7 and 9
8 - 11
-
benzaldehyde oxidation, more than 50% of maximal activity between pH 8 and 11
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.61
-
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
51740
Q83XU8
calculated from the predicted amino acid sequence
654394
52300
-
sequence analysis
675224
57000
-
SDS-PAGE; SDS-PAGE, recombinant protein
669112
126000
-
gel filtration
389581
160000
-
gel filtration
390254
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 51504, nucleotide sequence
?
-
x * 51682, nucleotide sequence
?
-
x * 51700, nucleotide sequence
?
-
x * 51682, nucleotide sequence
-
dimer
-
2 * 57000, SDS-PAGE
trimer
-
3 * 57000, SDS-PAGE, 3 * 57800, nucleotide sequence
trimer
Pseudomonas pseudoalcaligenes JS45
-
3 * 57000, SDS-PAGE, 3 * 57800, nucleotide sequence
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, 1 week, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, chromatography on DE52 cellulose, chromatography on DEAE-cellulofine, chromatography on DEAE-toyopearl; ammonium sulfate precipitation followed by 3 chromatography steps
-
by nickelnitrilotriacetic acid affinity chromatography; Ni-affinity chromatography
-
partial
-
erroneous identification as benzaldehyde dehydrogenase corrected
-
erroneously identified as benzaldehyde dehydrogenase
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli HB101
Q83XU8
expression as His-tag fusion protein in Escherichia coli Tuner (DE3); into the plasmid pDESTF1, which encodes an N-terminal six-His tag and is under control of the T7lac promoter, is used to transform Escherichia coli Tuner DE3
-
expressed in Escherichia coli carrying pDTG912, pDTG912/A, and pDTG912/B
-
gene hpcC
-
gene xylG from TOL plasmid of strain mt-2
-
NAH plasmid of Pseudomonas putida, carrying the coding gene nahI, cloned into Escherichia coli
-
gene dmpC
-
gene phnG
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
codG encoding 2-hydroxymuconic semialdehyde dehydrogenase shows a higher degree of similarity to those genes in classical bacteria