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Information on EC 1.2.1.13 - glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) and Organism(s) Arabidopsis thaliana and UniProt Accession P25856

for references in articles please use BRENDA:EC1.2.1.13
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Arabidopsis thaliana
UNIPROT: P25856 not found.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
glyceraldehyde-3-p dehydrogenase, nadp-glyceraldehyde-3-phosphate dehydrogenase, gapa1, nadp-gapdh, gapa-1, glyceraldehyde-3-dehydrogenase, nadp-gpd, photosynthetic gapdh, ov-gapdh, glyceraldehyde-p dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NADP-dependent GAPDH
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NADP-glyceraldehyde-3-phosphate dehydrogenase
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dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate phosphorylating)
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-
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GAPDH
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-
-
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glyceraldehyde 3-phosphate dehydrogenase (NADP)
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-
-
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glyceraldehyde phosphate dehydrogenase (nicotinamide adenine dinucleotide phosphate phosphorylating)
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-
-
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glyceraldehyde-P dehydrogenase
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-
-
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NAD(P)-GAPDH
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-
-
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NADP+-dependent G-3-P dehydrogenase
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-
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NADP-dependent GAPDH
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NADP-dependent glyceraldehyde phosphate dehydrogenase
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NADP-dependent glyceraldehydephosphate dehydrogenase
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NADP-glyceraldehyde phosphate dehydrogenase
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-
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NADP-glyceraldehyde-3-phosphate dehydrogenase
NADP-GPD
-
-
-
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NADP-triose phosphate dehydrogenase
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-
-
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NADPH-D-GA3P
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-
-
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photosynthetic GAPDH
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triosephosphate dehydrogenase (NADP+)
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
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oxidation
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-
-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde-3-phosphate:NADP+ oxidoreductase (phosphorylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-87-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
show the reaction diagram
-
-
-
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
show the reaction diagram
-
-
-
?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.67
sequence calculation, isozyme GAPA1
7.79
sequence calculation, isozyme GAPA2
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
very low activity
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
several GAPDH isozymes are targets of persulfidation, including NAD- and NADP-dependent GAPDH (GAPC1, GAPC2, GAPA1, and ALDH11A3). The in vitro assay of Arabidopsis leaf extracts in the presence of NaHS show an activity increase of 60% which is reversed by DTT
metabolism
posttranslational modifications promoted by NO (tyrosine nitration and S-nitrosation), H2S (persulfidation), and glutathione (glutathionylation), affect the cellular redox status through regulation of the NADP-dependent dehydrogenases. Major regulatory mechanisms of the NADPH-generating enzymes by NO and H2S in higher plants, with particular focus on the PTMs mediated by reactive nitrogen species (RNS) and and reactive sulfide species (RSS), overview
physiological function
the enzyme has different isozymes located in diverse subcellular compartments (chloroplasts, cytosol, mitochondria, and peroxisomes) which contribute to the NAPDH cellular pool. The NADPH/NADP+ ratio is a key indicator of cellular redox status. It is also a cofactor involved in many anabolic pathways and it supports some mechanisms of defense involved in ROS and RNS metabolism. The NADPH pool in cellular compartments is provided by several families of NADP-DHs located in different compartments which guarantee the stability and functioning of the cells. Regulation mechanisms, overview
malfunction
several GAPDH isozymes are targets of persulfidation, including NAD- and NADP-dependent GAPDH (GAPC1, GAPC2, GAPA1, and ALDH11A3). The in vitro assay of Arabidopsis leaf extracts in the presence of NaHS show an activity increase of 60% which is reversed by DTT
metabolism
posttranslational modifications promoted by NO (tyrosine nitration and S-nitrosation), H2S (persulfidation), and glutathione (glutathionylation), affect the cellular redox status through regulation of the NADP-dependent dehydrogenases. Major regulatory mechanisms of the NADPH-generating enzymes by NO and H2S in higher plants, with particular focus on the PTMs mediated by reactive nitrogen species (RNS) and reactive sulfide species (RSS), overview
physiological function
the enzyme has different isozymes located in diverse subcellular compartments (chloroplasts, cytosol, mitochondria, and peroxisomes) which contribute to the NAPDH cellular pool. The NADPH/NADP+ ratio is a key indicator of cellular redox status. It is also a cofactor involved in many anabolic pathways and it supports some mechanisms of defense involved in ROS and RNS metabolism. The NADPH pool in cellular compartments is provided by several families of NADP-DHs located in different compartments which guarantee the stability and functioning of the cells. Regulation mechanisms, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
G3PA1_ARATH
396
0
42490
Swiss-Prot
Chloroplast (Reliability: 2)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 36300, about, isozyme GAPA1, sequence calculation
?
x * 37700, about, isozyme GAPA2, sequence calculation
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of isoforms GapA-1, GapB and phosphoribulokinase and peptide Cp12-2 is co-ordinately regulated with the same organ specificity, all four genes being mostly expressed in leaf and flower stalk, less expressed in flower, and little or not expressed in roots and siliques. Expression in leaf is terminated during prolonged darkness or following sucrose treatment, and their transcripts decay with similar kinetics
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Marri, L.; Sparla, F.; Pupillo, P.; Trost, P.
Co-ordinated gene expression of photosynthetic glyceraldehyde-3-phosphate dehydrogenase, phosphoribulokinase, and CP12 in Arabidopsis thaliana
J. Exp. Bot.
56
73-80
2005
Arabidopsis thaliana (P25856), Arabidopsis thaliana (P25857), Arabidopsis thaliana
Manually annotated by BRENDA team
Corpas, F.J.; Gonzalez-Gordo, S.; Palma, J.M.
Nitric oxide and hydrogen sulfide modulate the NADPH-generating enzymatic system in higher plants
J. Exp. Bot.
72
830-847
2021
Arabidopsis thaliana (P25856), Arabidopsis thaliana (Q9LPW0)
Manually annotated by BRENDA team