Information on EC 1.17.7.1 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.17.7.1
-
RECOMMENDED NAME
GeneOntology No.
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
-
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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isoprenoid biosynthesis
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Metabolic pathways
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methylerythritol phosphate pathway II
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Terpenoid backbone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:oxidized ferredoxin oxidoreductase
An iron-sulfur protein found in plant chloroplasts and cyanobacteria that contains a [4Fe-4S] cluster [1]. Forms part of an alternative non-mevalonate pathway for isoprenoid biosynthesis. Bacteria have a similar enzyme that uses flavodoxin rather than ferredoxin (cf. EC 1.17.7.3). The enzyme from the plant Arabidopsis thaliana is active with photoreduced 5-deazaflavin but not with flavodoxin [1].
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
GcpE
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-
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gcpE gene product
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-
-
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IspG protein
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY
398144-56-4
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
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(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase converts 2-C-methyl-D-erythritol-2,4-cyclodiphosphate into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate in the penultimate step of the methyl-erythritol phosphate pathway for isoprene biosynthesis, pathway overview
metabolism
Q8DK70
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
metabolism
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the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
metabolism
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the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
physiological function
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GcpE/IspG converts 2-C-methyl-D-erythritol-2,4-cyclodiphosphate into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate for isoprene biosynthesis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
Q8DK70
-
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
-
-
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
Q8DK70
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
F4K0E8
the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
-
the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
F4K0E8
in the presence of light and via ferredoxin the electron flow from photosynthesis can directly provide the electrons required for the GcpE catalyzed reaction
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
Q8DK70
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
F4K0E8
the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
-
the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
[4Fe-4S]2 center
Q8DK70
[4Fe-4S]2 metalloprotein
PDB
SCOP
CATH
ORGANISM
Aquifex aeolicus (strain VF5)
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant GcpE protein is purified by an N-terminal His6 tag and reconstituted as a [4Fe-4S]2 metalloprotein
Q8DK70
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
Q8DK70
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced by light
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