Information on EC 1.17.7.1 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.17.7.1
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RECOMMENDED NAME
GeneOntology No.
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
show the reaction diagram
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(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
show the reaction diagram
reaction mechanism modelling, overview
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Metabolic pathways
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methylerythritol phosphate pathway
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Terpenoid backbone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:oxidized ferredoxin oxidoreductase
An iron-sulfur protein that contains a [4Fe-4S] cluster [1,2]. Forms, in the reverse direction, part of an alternative non-mevalonate pathway for isoprenoid biosynthesis that is found in most bacteria and in plant chloroplasts [4]. The enzyme from the plant Arabidopsis thaliana is active with photoreduced 5-deazaflavin but not with the flavodoxin/flavodoxin reductase/NADPH system that can be used as an electron donor by Escherichia coli [2]. Metabolites derived from isoprenoids play important roles in systems such as electron transport, photosynthesis, plant defense responses, hormonal regulation of development and membrane fluidity [1].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase
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1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate-synthase
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GCEP
Solanum lycopersicum Mill.
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GcpE
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gcpE gene product
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HDS
Solanum lycopersicum Mill.
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hydroxymethylbutenyl 4-diphosphate synthase
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hydroxymethylbutenyl 4-diphosphate synthase
Solanum lycopersicum Mill.
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IspG protein
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IspG-protein
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CAS REGISTRY NUMBER
COMMENTARY
398144-56-4
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
gene ispG
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Manually annotated by BRENDA team
gene ispG
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Manually annotated by BRENDA team
Solanum lycopersicum Mill.
Mill.
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Manually annotated by BRENDA team
; gene gcpE
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Manually annotated by BRENDA team
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
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NADH flavin oxidoreductase protein, can affect the expression level of the ispG gene
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PDB
SCOP
CATH
ORGANISM
Aquifex aeolicus (strain VF5)
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C270S
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no enzymatic activity. Less than 23% sulfide content and 6% iron content, compared with wild-type
C273S
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no enzymatic activity. Less than 23% sulfide content and 6% iron content, compared with wild-type
C306S
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no enzymatic activity. Less than 23% sulfide content and 6% iron content, compared with wild-type
additional information
Q8DK70, -
simultaneous expression of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase and ferredoxin I in Escherichia coli shows strong interaction of both proteins. Ferredoxin I is able to transfer electrons to the synthase