Information on EC 1.17.7.1 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.17.7.1
-
RECOMMENDED NAME
GeneOntology No.
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methylerythritol phosphate pathway II
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isoprenoid biosynthesis
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Terpenoid backbone biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
(E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:oxidized ferredoxin oxidoreductase
An iron-sulfur protein found in plant chloroplasts and cyanobacteria that contains a [4Fe-4S] cluster [1]. Forms part of an alternative non-mevalonate pathway for isoprenoid biosynthesis. Bacteria have a similar enzyme that uses flavodoxin rather than ferredoxin (cf. EC 1.17.7.3). The enzyme from the plant Arabidopsis thaliana is active with photoreduced 5-deazaflavin but not with flavodoxin [1].
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
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GcpE
-
-
-
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gcpE gene product
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-
-
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GcpE/IspG
ambiguous
IspG protein
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY
398144-56-4
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
metabolism
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
metabolism
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the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
metabolism
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(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase converts 2-C-methyl-D-erythritol-2,4-cyclodiphosphate into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate in the penultimate step of the methyl-erythritol phosphate pathway for isoprene biosynthesis, pathway overview
physiological function
-
GcpE/IspG converts 2-C-methyl-D-erythritol-2,4-cyclodiphosphate into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate for isoprene biosynthesis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
-
-
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
-
the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
in the presence of light and via ferredoxin the electron flow from photosynthesis can directly provide the electrons required for the GcpE catalyzed reaction
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
Q8DK70
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
F4K0E8
the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
-
?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
show the reaction diagram
-
the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
[4Fe-4S]2 center
[4Fe-4S]2 metalloprotein
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aquifex aeolicus (strain VF5)
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant GcpE protein is purified by an N-terminal His6 tag and reconstituted as a [4Fe-4S]2 metalloprotein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced by light
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