Information on EC 1.17.5.2 - caffeine dehydrogenase

for references in articles please use BRENDA:EC1.17.5.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Pseudomonas

EC NUMBER
COMMENTARY hide
1.17.5.2
-
RECOMMENDED NAME
GeneOntology No.
caffeine dehydrogenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
caffeine + ubiquinone + H2O = 1,3,7-trimethylurate + ubiquinol
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
caffeine degradation V (bacteria, via trimethylurate)
-
-
Caffeine metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
caffeine:ubiquinone oxidoreductase
This enzyme, characterized from the soil bacterium Pseudomonas sp. CBB1, catalyses the incorporation of an oxygen atom originating from a water molecule into position C-8 of caffeine. The enzyme utilizes short-tail ubiquinones as the preferred electron acceptor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
caffeine + ubiquinone-0
trimethyl uric acid + ubiquinol-0
show the reaction diagram
theobromine + ubiquinone-0
? + ubiquinol-0
show the reaction diagram
theophylline + ubiquinone-0
? + ubiquinol-0
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ubiquinone-0
-
preferred electron acceptor
additional information
-
enzyme is not NAD(P)+-dependent
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0037
Caffeine
-
pH 7.5, 35C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.167
Caffeine
-
pH 7.5, 35C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1530
-
pH 7.5, 35C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
52% of maximum activity
8
-
47% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66
-
or above, activity increases linearly from 25 to 66C, which is the highest temperature allowed by the experimental conditions
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
-
beta subunit
40000
-
1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE
158000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
-
30 min, 77% residual activity
70
-
30 min, 11% residual activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, 5 mM potassium phosphate buffer pH 7.5, enzyme loses activity after a few days
-
Show AA Sequence (198 entries)
Please use the Sequence Search for a specific query.