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Enzyme Nomenclature
Information on EC 1.14.99.38 - cholesterol 25-hydroxylase
Word Map on EC 1.14.99.38
- 1.14.99.38
- hepatic
-
pregnane
-
cyp2b10
-
xenobiotic
-
androstane
- 25-hydroxycholesterol
-
drug-metabolizing
-
oxysterols
-
cyp2c29
-
cyp3a13
-
lithocholic
-
apap-induced
-
pxr-null
-
pxr-mediated
-
cyp2a4
- ugt1a1
- medicine
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The expected taxonomic range for this enzyme is: Tetrapoda
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EC NUMBER 
COMMENTARY 
1.14.99.38
-
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RECOMMENDED NAME
GeneOntology No.
cholesterol 25-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
cholesterol,hydrogen-donor:oxygen oxidoreductase (25-hydroxylating)
Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P-450. Instead, it uses diiron cofactors to catalyse the hydroxylation of hydrophobic substrates [1]. The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues [4,5]. In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs).
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CAS REGISTRY NUMBER
COMMENTARY
60202-07-5
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
additional information
malfunction
patients with hereditary spastic paresis, a rare inherited disease with mutations in the CYP7B1 gene, have highly elevated levels of 25- and 27-hydroxycholesterol but normal levels of cholesterol and bile acids
malfunction
mice with a disruption in the cholesterol 25-hydroxylase gene regulate cholesterol metabolism normally. Ch25h-/- mice have higher levels of mRNAs coding for immunoglobulin A and immunoglobulin J. Disruption in the Cyp7b1 gene leads to significantly increased levels of 25-hydroxycholesterol in the circulation, and to significantly lower levels of IgA compared to wild-type mice
malfunction
-
loss of function of Ch25h leads to susceptibility to viral infections in vitro
physiological function
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
-
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
-
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
-
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
-
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
-
cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis
physiological function
formation of 25-hydroxycholesterol in liver mitochondria is dependent on CYP27A1, and the 25-hydroxycholesterol formed is subsequently sulfated by the insulin-regulated cytosolic sulfotransferase 2B1b. 25-Hydroxycholesterol is, however, a weak activator of the liver X receptors. 25-Hydroxycholesterol is of great importance for maintaining cholesterol homeostasis. 25-Hydroxycholesterol induces the secretion of the proinflammatory cytokine IL-8
physiological function
important role for 25-hydroxycholesterol in the immune system. High concentrations of 25-hydroxycholesterol induce apoptosis in a mouse oligodendrocyte cell line 158N in an liver X receptor-independent manner. Treatment of these cells with 25-hydroxycholesterol results in enhanced secreted phospholipase A2 activity
physiological function
-
formation of 25-hydroxycholesterol in liver mitochondria is dependent on CYP27A1, and the 25-hydroxycholesterol formed is subsequently sulfated by the insulin-regulated cytosolic sulfotransferase 2B1b. 25-Hydroxycholesterol decreases the level of cytoplasmic IkBa and increases the nuclear NFkB level, its sulfated form has the opposite effects
physiological function
-
cholesterol-25-hydroxylase acts as a broadly antiviral interferon-stimulated gene. CH25H converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol. 25-Hydroxycholesterol treatment in cultured cells broadly inhibits growth of enveloped viruses including VSV, HSV, HIV, and MHV68 and acutely pathogenic EBOV, RVFV, RSSEV, and Nipah viruses. The compound inhibits cell entry of viruses HIV and VSV, and inhibits viral protein-mediated cell-cell membrane fusion of virus NIV. 25-Hydroxycholesterol directly modifies membranes
additional information
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Ch25h expression is controlled primarily by type I IFN signaling, type1 IFN-mediated Ch25h expression is IFNR-dependent, overview
additional information
the high concentrations of vitamin D3 metabolites required for upregulation of the cholesterol 25-hydroxylase gene makes it unlikely that these metabolites are important regulators of cholesterol 25-hydroxylase in vivo
additional information
25-hydroxycholesterol does not stimulate the sPLA2-IIA promoter in the mouse Schwann cell line MSC80
additional information
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Ch25h expression is controlled primarily by type I IFN signaling, type1 IFN-mediated Ch25h expression is IFNR-dependent, overview
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
genetic variability in the set of cholesterol metabolism genes: cholesterol 25-hydroxylase, cholesterol 24-hydroxylase and ATP-binding cassette transporter A1 does not influence the development of Alzheimer‘s disease
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
the reaction might be catalyzed by CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
-
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
the reaction might be catalyzed by CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
testicular macrophagers play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
expression of 25-hydroxylase mRNA and production of 25-hydroxycholesterol by cultured testicular macrophages is significantly inhibited by testosterone at 0.01 mg/ml
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-
?
additional information
?
-
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
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-
-
additional information
?
-
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
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-
-
additional information
?
-
-
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
-
-
-
additional information
?
-
-
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
genetic variability in the set of cholesterol metabolism genes: cholesterol 25-hydroxylase, cholesterol 24-hydroxylase and ATP-binding cassette transporter A1 does not influence the development of Alzheimer‘s disease
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
O95992
the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
P05177, P08684, P10635, P11712
lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
P05177, P08684, P10635, P11712
lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
P05177, P08684, P10635, P11712
lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
P05177, P08684, P10635, P11712
production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Q02318
the reaction might be catalyzed by CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
-
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Q9Z0F5
the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Q64459
the reaction might be catalyzed by CYP3A4
-
-
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
testicular macrophagers play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol
-
-
?
additional information
?
-
Q02318
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
-
-
-
additional information
?
-
Q64459
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
-
-
-
additional information
?
-
-
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
-
-
-
additional information
?
-
-
25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe
Fe2+
Fe
-
diiron-containing enzyme. The iron is present as Fe-O-Fe or Fe-OH-Fe and is coordinated with clusters of conserved histidine residues
Fe
-
diiron-containing enzyme. The iron is present as Fe-O-Fe or Fe-OH-Fe and is coordinated with clusters of conserved histidine residues
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
desmosterol
a potent inhibitor of CH25H; a potent inhibitor of CH25H; a potent inhibitor of CH25H
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
incubation with LPS derived from Escherichia coli or Salmonella enterica induces CH25H mRNA
-
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
patients with chronic obstructive pulmonary disease have increased expression of cholesterol 25-hydroxylase in the lung
-
testicular, 10-day-old animals have the highest steady-state levels of message
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
additional information
-
quantitative enzyme expression analysis in macrophages and dendritic cells, overview
additional information
-
quantitative enzyme expression analysis in macrophages and dendritic cells, overview
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
-
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
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transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
-
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
-
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
-
transmembrane enzyme, prediction of transmembrane structures for CH25H subunits, overview
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
gene CH25H, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
-
intronless gene CH25H is located on chromosome 1, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
intronless gene CH25H is located on chromosome 10, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
-
intronless gene CH25H is located on chromosome 19, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
-
intronless gene CH25H is located on chromosome 26, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
intronless gene CH25H is located on chromosome 6, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
-
intronless gene CH25H is located on chromosome 9, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
-
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
-
intronless gene CH25H is located on chromosome 1, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
intronless gene CH25H is located on chromosome 1, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
intronless gene CH25H is located on chromosome 26, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
intronless gene CH25H is located on chromosome 26, DNA and amino acid sequence deterination and analysis, sequence comparisons, gene structures and tandem locations for the human CH25H and LIPA, EC 3.1.1.13, genes on chromosome 10
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activation of the innate immune system by TLR ligands induces 25-hydroxycholesterol production which results in suppression of IgA class switching in B cells
both IFN-alpha- and IFN-gamma induce the enzyme in bone marrow-derived macrophages
-
induction of Ch25h is JAK- and STAT1-dependent, overview
induction of CYP3A by pregnenolone-16 alpha-carbonitrile causes accumulation of 25-hydroxycholesterol in cell line AML12 derived from mouse liver, the induction is not suppressed by the addition of desmosterol. Although the transcription of Ch25h is also upregulated by addition of pregnenolone-16 alpha-carbonitrile, the protein level of CH25H is not elevated. Desmosterol does not affect the expression of cellular CYP3A protein, but CH25H protein level is obviously decreased by desmosterol treatment
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
in cells overexpressing human recombinant CYP3A4, the activity of cholesterol 25-hydroxylation is higher than that of cholesterol 4beta-hydroxylation, a known marker activity of CYP3A4
additional information
in cells overexpressing human recombinant CYP3A4, the activity of cholesterol 25-hydroxylation is higher than that of cholesterol 4beta-hydroxylation, a known marker activity of CYP3A4
additional information
in cells overexpressing human recombinant CYP3A4, the activity of cholesterol 25-hydroxylation is higher than that of cholesterol 4beta-hydroxylation, a known marker activity of CYP3A4
additional information
in cells overexpressing human recombinant CYP3A4, the activity of cholesterol 25-hydroxylation is higher than that of cholesterol 4beta-hydroxylation, a known marker activity of CYP3A4
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
medicine
-
genetic variability in the set of cholesterol metabolism genes: cholesterol 25-hydroxylase, cholesterol 24-hydroxylase and ATP-binding cassette transporter A1 does not influence the development of Alzheimer‘s disease
medicine
-
patients with Alzheimer’s disease show high expression of cholesterol 25-hydroxylase gene in specifically vulnerable brain regions. The common enzyme haplotype CH25Hchi4 is a putative susceptibility factor for sporadic Alzheimer’s disease
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.99.38)
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