Information on EC 1.14.99.19 - plasmanylethanolamine desaturase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.14.99.19
-
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RECOMMENDED NAME
GeneOntology No.
plasmanylethanolamine desaturase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + AH2 + O2 = O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + A + 2 H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ether lipid metabolism
-
-
Metabolic pathways
-
-
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SYSTEMATIC NAME
IUBMB Comments
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine,hydrogen-donor:oxygen oxidoreductase
Requires NADPH or NADH. May involve cytochrome b5. Requires Mg2+ and ATP.
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CAS REGISTRY NUMBER
COMMENTARY hide
39391-13-4
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
plasmanylethanolamine desaturase is a key enzyme for the biosynthesis of one of the most abundant phospholipidsin the body, the plasmalogens. Ether lipid metabolism including biosynthesis and degradation of platelet-activating factor and plasmalogens, overview
additional information
-
the enzyme is a defined enzyme without assigned sequence, these kind of enzymes are known as orphan enzymes
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-hexadecyl-2-acyl glycero-3-phosphoethanolamine + NADH + O2
?
show the reaction diagram
-
in situ in the microsomes via acylation of 1-hexadecyl-glycero-3-phosphoethanolamine
-
-
?
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + AH2 + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + A + 2 H2O
show the reaction diagram
-
i.e. plasmanylethanolamine
i.e. plasmenylethanolamine
-
?
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
show the reaction diagram
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + reduced cytochrome b5 + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized cytochrome b5 + 2 H2O
show the reaction diagram
-
i.e. plasmanylethanolamine, the reaction involves reaction involving cytochrome b5 reductase, molecular oxygen and a reduction equivalent
i.e. plasmenylethanolamine
-
?
O-1-hexadecyl-rac-glycero-3-phosphoethanolamine + NADPH + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + AH2 + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + A + 2 H2O
show the reaction diagram
-
i.e. plasmanylethanolamine
i.e. plasmenylethanolamine
-
?
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome b5
-
NADPH
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
cholate
-
weak inhibition
oleate
-
weak inhibition
p-chloromercuribenzoate
-
-
taurocholate
-
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
catalase
-
heat-lable factor of high molecular weight
-
contained in the 100000 * g supernatant
-
Mg2+
-
stimulates the conversion by a factor of 2
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000028
-
microsomes plus soluble fraction, enzyme requires a heat-labile factor of high molecular weight contained in the soluble fraction
0.0000057 - 0.0000064
-
two-dimensional TLC method
0.0000066
-
hydrolysis followed by solvent partition
0.000025
-
tumor cells of rat on fat-free diet
0.000029
-
tumor cells of rat on normal diet
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
-
phosphate buffer
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Fischer R-3259 sarcoma
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity loss at -23°C, should be used freshly
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-23°C, postmitochondrial supernatant stable for two weeks
-
-23°C, supernatant
-
-70°C, TES buffer, 0.25 M sucrose, 1 mM EDTA
-
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.99.19)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)