Any feedback?
Information on EC 1.14.99.1 - prostaglandin-endoperoxide synthase and Organism(s) Ovis aries and UniProt Accession P79208
for references in articles please use BRENDA:EC1.14.99.1Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.14.99.1 prostaglandin-endoperoxide synthaseIUBMB Comments
This enzyme acts both as a dioxygenase and as a peroxidase.
Specify your search results
Word Map
- 1.14.99.1
-
arachidonic
- indomethacin
- necrosis
-
nsaid
- tnf
- lipopolysaccharide
- endothelial
-
nonsteroidal
- celecoxib
- artery
-
thromboxane
- aspirin
- lps
- lipoxygenase
- pain
- cardiovascular
-
lps-induced
- platelet
-
prostanoids
- agonist
- gastrointestinal
- mapks
- phospholipase
- gastric
- carcinogenesis
-
eicosanoids
-
leukotriene
- ulcer
- arthritis
-
analgesic
- prostacyclin
- nf-kappab
-
lps-stimulated
- colorectal
- angiogenesis
- edema
- tnf-alpha
- ibuprofen
-
vasodilator
- il-1beta
- osteoarthritis
-
nonselective
- factor-alpha
-
chemopreventive
-
lipopolysaccharide-induced
-
factor-kappa
-
endothelium-dependent
-
vasoconstriction
-
l-name
-
endothelium-derived
- medicine
The taxonomic range for the selected organisms is: Ovis aries
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
cox-2, cyclooxygenase, cyclooxygenase-2, cox-1, cyclooxygenase 2, ptgs2, pghs-2, cyclooxygenase-1, prostaglandin synthetase, pghs-1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PGHS-2
prostaglandin endoperoxide synthase-2
(PG)H synthase
-
-
-
-
cyclooxygenase
fatty acid cyclooxygenase
-
-
-
-
PG synthetase
-
-
-
-
PGHS
PGHS-1
PHS-1
PHS is a dual-function enzyme, having a cyclooxygenase and a hydroperoxidase component
prostaglandin endoperoxide synthase-1
prostaglandin endoperoxide synthetase
-
-
-
-
prostaglandin G/H synthase
-
-
-
-
prostaglandin H synthase
prostaglandin H2 synthase
prostaglandin synthase
-
-
-
-
prostaglandin synthase-2
-
-
-
-
prostaglandin synthetase
-
-
-
-
prostaglandin-endoperoxide synthase
prostaglandin-H-synthase
synthase, prostaglandin
-
-
-
-
PGHS-2
one of two isoforms found in mammalian cells, produced in response to certain stimuli, such as cytokines and growth factors
cyclooxygenase
-
-
-
-
PGHS-1
one of two isoforms found in mammalian cells, always present and provides their normal functioning
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
arachidonate + reduced acceptor + 2 O2 = prostaglandin H2 + acceptor + H2O
mechanism, enzyme acts both as dioxygenase and as peroxidase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
reduction
redox reaction
-
-
-
-
oxidation
reduction
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate,hydrogen-donor:oxygen oxidoreductase
This enzyme acts both as a dioxygenase and as a peroxidase.
CAS REGISTRY NUMBER
COMMENTARY
39391-18-9
-
9055-65-6
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
arachidonic acid + 2 O2
prostaglandin G2
cyclooxygenase reaction, arachidonic acid as electron donor
-
-
?
linoleic acid + AH2 + O2
9-hydroxyoctadecadienoic acid + 13-hydroxyoctadecadienoic acid + ?
-
-
-
?
arachidonate + electron donor + O2
prostaglandin H2 + oxidized electron donor + H2O
-
-
-
-
?
arachidonate + reduced acceptor + O2
prostaglandin H2 + acceptor + H2O
-
-
-
?
arachidonic acid + 2 O2
prostaglandin G2
cyclooxygenase reaction, arachidonic acid as electron donor
-
-
?
arachidonic acid + AH2 + 2 O2
15-hydroperoxy-9alpha,11alpha-peroxidoprosta-5,13-dienoic acid + A + H2O
-
-
-
?
linoleic acid + AH2 + O2
9-hydroxyoctadecadienoic acid + 13-hydroxyoctadecadienoic acid + ?
-
-
-
?
arachidonate + AH2 + O2
prostaglandin H2 + A + H2O
-
-
-
-
?
arachidonate + AH2 + O2
prostaglandin H2 + A + H2O
the reaction comprises two steps: dioxygenation of arachidonate to yield prostaglandin G2 containing both a 9-11 endoperoxide and a 15-peroxide group, and a peroxidase reaction, which converts prostaglandin G2 to prostaglandin H2 where the 15-peroxide is reduced to an alcohol
-
-
?
additional information
?
-
prostaglandin synthesis within the fetal central nervous system is critical for the the modulation of hypotension-induced fetal ACTH secretion involving PGHS-2, overview
-
-
?
additional information
?
-
prostaglandin synthesis within the fetal central nervous system is critical for the the modulation of hypotension-induced fetal ACTH secretion involving PGHS-2, overview
-
-
?
additional information
?
-
-
prostaglandin synthesis within the fetal central nervous system is critical for the the modulation of hypotension-induced fetal ACTH secretion involving PGHS-2, overview
-
-
?
additional information
?
-
-
electron donors used by hydroperoxidase: phenylbutazone, sulindac
-
-
?
additional information
?
-
-
electron donors used by hydroperoxidase: phenylbutazone, sulindac
-
-
?
additional information
?
-
-
xenobiotics such as benzo(a)pyrene cannot act as electron donor, but undergo cooxydation during hydroperoxidase reaction
-
-
?
additional information
?
-
-
functional differentiation of cyclooxygenase and peroxidase activities by trypsin treatment
-
-
?
additional information
?
-
-
cooxidation of: 4-chloroaniline to yield N-(4-chlorophenyl)-hydroxylamine and 1-chloro-4-nitrosobenzene
-
-
?
additional information
?
-
-
also catalyzed: transformation of arachidonic acid into prostaglandin E2, prostaglandin F2 alpha and 12-hydroxy-5,8,10-heptadecatrienoic acid
-
-
?
additional information
?
-
-
enzyme has a central position in prostanoic metabolism: first step in formation of prostaglandins and thromboxanes, the conversion of arachidonic acid to prostaglandin endoperoxides G and H
-
-
?
additional information
?
-
-
maximal values of the initial reaction rate and yield of the product are attained at oxygen concentration 0.05 mM
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
arachidonate + reduced acceptor + O2
prostaglandin H2 + acceptor + H2O
-
-
-
?
arachidonate + AH2 + O2
prostaglandin H2 + A + H2O
-
-
-
-
?
additional information
?
-
prostaglandin synthesis within the fetal central nervous system is critical for the the modulation of hypotension-induced fetal ACTH secretion involving PGHS-2, overview
-
-
?
additional information
?
-
prostaglandin synthesis within the fetal central nervous system is critical for the the modulation of hypotension-induced fetal ACTH secretion involving PGHS-2, overview
-
-
?
additional information
?
-
-
prostaglandin synthesis within the fetal central nervous system is critical for the the modulation of hypotension-induced fetal ACTH secretion involving PGHS-2, overview
-
-
?
additional information
?
-
-
enzyme has a central position in prostanoic metabolism: first step in formation of prostaglandins and thromboxanes, the conversion of arachidonic acid to prostaglandin endoperoxides G and H
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
epinephrine-hydrogentartrate can servve as cofactor
-
additional information
epinephrine-hydrogentartrate can servve as cofactor
-
additional information
epinephrine-hydrogentartrate can serve as cofactor
-
additional information
epinephrine-hydrogentartrate can serve as cofactor
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn3+
-
substitution of ferric heme by MnIII protoporphyrin IX greatly diminishes the peroxidase activity, but has little effect on the cyclooxygenase activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
DCM-extract of Angelicae dahuricae radix
38.8% inhibition of PGHS-2
-
DCM-extract of Angelicae sinsesis radix
55.8% inhibition of PGHS-2
-
DCM-extract of Atractylodis lanceae rhizoma
50.3% inhibition of PGHS-2
-
DCM-extract of Atractylodis macrocephalae rhizoma
47.0% inhibition of PGHS-2
-
DCM-extract of Notopterygii rhizoma seu radix
-2.1% inhibition of PGHS-2
-
DCM-extract of Piperis sarmentosi herba
10.1% inhibition of PGHS-2
-
n-hexane extract of Angelicae dahuricae radix
42.4% inhibition of PGHS-2
-
n-hexane extract of Angelicae sinsesis radix
61.5% inhibition of PGHS-2
-
n-hexane extract of Atractylodis lanceae rhizoma
68.3% inhibition of PGHS-2
-
n-hexane extract of Atractylodis macrocephalae rhizoma
48.9% inhibition of PGHS-2
-
n-hexane extract of Notopterygii rhizoma seu radix
64.9% inhibition of PGHS-2
-
n-hexane extract of Piperis sarmentosi herba
65.0% inhibition of PGHS-2
-
n-hexane extract of Zanthoxyli pericarpium
24.9% inhibition of PGHS-2
-
5-amino-2-hydroxy-N-(propan-2-yl)benzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 7.6fold selectivity for isoform Cox-2 over Cox-1
-
5-amino-N-cyclohexyl-2-hydroxybenzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 44fold selectivity for isoform Cox-2 over Cox-1
-
5-amino-N-hexyl-2-hydroxybenzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 44fold selectivity for isoform Cox-2 over Cox-1
-
5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxy-N-(4-methylphenyl)benzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 29.7fold selectivity for isoform Cox-2 over Cox-1
-
Acetylsalicylic acid
-
inhibition of oxygenase activity by acetylating a serine residue of the enzyme
DCM-extract of Angelicae dahuricae radix
0.1% inhibition of PGHS-1
-
DCM-extract of Angelicae sinsesis radix
75.0% inhibition of PGHS-1
-
DCM-extract of Atractylodis lanceae rhizoma
46.9% inhibition of PGHS-1
-
DCM-extract of Atractylodis macrocephalae rhizoma
58.6% inhibition of PGHS-1
-
DCM-extract of Notopterygii rhizoma seu radix
42.6% inhibition of PGHS-1
-
DCM-extract of Piperis sarmentosi herba
47.2% inhibition of PGHS-1
-
N-butyl-5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxybenzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 135fold selectivity for isoform Cox-2 over Cox-1
-
N-cyclohexyl-5-[(E)-[(2,5-dihydroxyphenyl)methylidene]amino]-2-hydroxybenzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 120fold selectivity for isoform Cox-2 over Cox-1
-
N-cyclohexyl-5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxybenzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 114fold selectivity for isoform Cox-2 over Cox-1
-
n-hexane extract of Angelicae dahuricae radix
52.5% inhibition of PGHS-1
-
n-hexane extract of Angelicae sinsesis radix
73.0% inhibition of PGHS-1
-
n-hexane extract of Atractylodis lanceae rhizoma
67.4% inhibition of PGHS-1
-
n-hexane extract of Atractylodis macrocephalae rhizoma
46.1% inhibition of PGHS-1
-
n-hexane extract of Notopterygii rhizoma seu radix
69.6% inhibition of PGHS-1
-
n-hexane extract of Piperis sarmentosi herba
52.4% inhibition of PGHS-1
-
n-hexane extract of Zanthoxyli pericarpium
48.5% inhibition of PGHS-1
-
N-hexyl-5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxybenzamide
-
5-aminosalicylic acid derivative, additionally inhibits lipoxygenase Lox-5. 145fold selectivity for isoform Cox-2 over Cox-1
-
O2
-
the cyclooxygenase reaction is inhibited by an excess of dissolved oxygen, 0.5 mM O2 causes twofold decrease in the initial rate and maximal yield
additional information
nimesulide inhibits PGHS-2 turnover in most brain regions
-
additional information
nimesulide inhibits PGHS-2 turnover in most brain regions
-
additional information
-
nimesulide inhibits PGHS-2 turnover in most brain regions
-
additional information
guava leaf extract inhibits the catalytic activity of the PGHS-1 and PGHS-2 isoforms using linoleic acid as substrate
-
additional information
guava leaf extract inhibits the catalytic activity of the PGHS-1 and PGHS-2 isoforms using linoleic acid as substrate
-
additional information
isozyme PGHS-2 is hardly inhibited by ellagic acid
-
additional information
isozyme PGHS-2 is hardly inhibited by ellagic acid
-
additional information
guava leaf extract inhibits the catalytic activity of the PGHS-1 and PGHS-2 isoforms using linoleic acid as substrate
-
additional information
guava leaf extract inhibits the catalytic activity of the PGHS-1 and PGHS-2 isoforms using linoleic acid as substrate
-
additional information
isozyme PGHS-1 is hardly inhibited by ellagic acid
-
additional information
isozyme PGHS-1 is hardly inhibited by ellagic acid
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
peroxynitrite
-
peroxidase activity, MS-analysis reveals that tyrosine 385 is a target for nitration by ONOO- only when heme is present
additional information
-
aqueous extracts of Chromoleana odorata, commonly used in traditional medicine as antiinflammatory drug against pains or as cataplasm to stop hemorrhage in Ivory Coast, the essential oil extracted from the fresh leaves activates the cyclooxygenase activity of the PGHS, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
the cyclooxygenase reaction shows Michaelis-Menten kinetics over a wide range of oxygen concentrations in the absence of electron donor. Kinetics analysis, overview
-
0.0027
arachidonate
purified isoform PGHS-1, method: coupled, homo-vanilic acid
0.011
arachidonate
purified isoform PGHS-1, method: coupled, homo-vanilic acid
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00298
5-amino-2-hydroxy-N-(propan-2-yl)benzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0083
5-amino-N-cyclohexyl-2-hydroxybenzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0097
5-amino-N-hexyl-2-hydroxybenzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0098
5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxy-N-(4-methylphenyl)benzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0135
N-butyl-5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxybenzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0132
N-cyclohexyl-5-[(E)-[(2,5-dihydroxyphenyl)methylidene]amino]-2-hydroxybenzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0126
N-cyclohexyl-5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxybenzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0145
N-hexyl-5-[[(2,5-dihydroxyphenyl)methyl]amino]-2-hydroxybenzamide
Ovis aries
-
pH 7.4, temperature not specified in the publication
-
0.0186
peroxynitrite
Ovis aries
-
cyclooxygenase activity, peroxynitrite is incubated with the resting enzyme and 30 s later, activity and protein nitration is analyzed
0.0222
peroxynitrite
Ovis aries
-
peroxidase activity, peroxynitrite is incubated with the resting enzyme and 30 s later, activity and protein nitration is analyzed
0.03
peroxynitrite
Ovis aries
-
cyclooxygenase activity, enzyme is incubated with peroxynitrite in the presence of peroxides and arachidonic acid
0.0388
peroxynitrite
Ovis aries
-
peroxidase activity, enzyme is incubated with peroxynitrite in the presence of peroxides and arachidonic acid
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
PGHS-2 has lowest abundance in brain stem and pituitary gland
PGHS-2 has highest abundance in hippocampus and cerebral cortex
PGHS-2 has highest abundance in hippocampus and cerebral cortex
PGHS-2 has lowest abundance in brain stem and pituitary gland
PGSHS-1 has lowest abundance in brain stem and pituitary gland
PGSHS-1 has highest abundance in hippocampus and cerebral cortex
PGSHS-1 has highest abundance in hippocampus and cerebral cortex
PGSHS-1 has lowest abundance in brain stem and pituitary gland
-
-
438369, 438371, 438374, 438379, 438381, 438383, 438384, 438385, 438387, 438388, 659705, 671047, 673508, 673809, 675273, 713706
additional information
relationship of PGHS-2 and PGHS-1 mRNA and activity in brain tissues, overview
additional information
relationship of PGHS-2 and PGHS-1 mRNA and activity in brain tissues, overview
additional information
-
relationship of PGHS-2 and PGHS-1 mRNA and activity in brain tissues, overview
additional information
-
enzyme can be associated with endoplasmic reticulum, nuclear envelope and plasma membrane even within the same cell
additional information
relationship of PGHS-2 and PGHS-1 mRNA and activity in brain tissues, overview
additional information
relationship of PGHS-2 and PGHS-1 mRNA and activity in brain tissues, overview
additional information
-
relationship of PGHS-2 and PGHS-1 mRNA and activity in brain tissues, overview
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
enzyme can be associated with endoplasmic reticulum, nuclear envelope and plasma membrane even within the same cell
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
the enzyme catalyzes the committed step in prostaglandin biosynthesis
physiological function
additional information
-
aqueous extracts of Chromoleana odorata, commonly used in traditional medicine as antiinflammatory drug against pains or as cataplasm to stop hemorrhage in Ivory Coast, the essential oil extracted from the fresh leaves activates the cyclooxygenase activity of the PGHS, overview
physiological function
the bi-functional enzyme with cyclooxygenase and peroxidase activities plays a central role in the inflammatory response, pain, and blood clotting
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PGH2_SHEEP
603
0
68969
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
65620
-
amino acid sequence deduced from nucleotide sequence of cDNA, MW of unglycosylated enzyme
70000
72000
72000
-
x * 72000, SDS-PAGE, oxygenase and peroxidase activity are present in a single polypeptide chain, in nonionic detergent the enzyme is a dimer of 2 identical subunits
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 72000, SDS-PAGE, oxygenase and peroxidase activity are present in a single polypeptide chain, in nonionic detergent the enzyme is a dimer of 2 identical subunits
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor-diffusion method, 2.0 A resolution X-ray crystal structure of the prostaglandin H2 synthase-1 in complex with alpha-methyl-4-biphenylacetic acid, a defluorinated analog of the steroidal anti.inflammatory drug flurbiproten
-
the 2.0 A resolution crystal structure of the MnIII form of ovine prostaglandin H2 synthase-1 is described
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Arg120Glu
-
Arg120 important for interaction with substrate and with inhibitors containing a free carboxylic acid moiety
Cys313Ser
-
cyclooxygenase and peroxidase activity reduced by 80-90%, no significant effect on inhibition, dimer formation, glycosylation
Cys540Ser
-
cyclooxygenase and peroxidase activity reduced by 80-90%, no significant effect on inhibition, dimer formation, glycosylation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
N-octyl-beta-D-glucopyranoside, Tween-20, CHAPS, and maltoside are used for solubilization of prostaglandin H2 synthase-1, frozen microsomal extracts are thawed and purified using a fast-flow DEAE-Sepharose and a S-300 column
-
the apo form of ovine PGHS isoform-1 is purified from ram seminal vesicles, the holoenzyme is reconstituted by adding MnIII-PPIX to the enzyme
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the hippocampus exhibits increases in PGHS-2 mRNA after 80 days of gestational age, brainstem and cortex PGHS-2 exhibit robust increases in mRNA postpartum, cerebellar PGHS-2 mRNA is upregulated at 120 days of gestational age
brainstem PGHS-1 exhibits robust increases in mRNA postpartum, cerebellar PGHS-1 mRNA is upregulated at 120 days of gestational age
the hippocampus exhibits decreases in PGHS-1 mRNA after 80 days of gestational age
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
DeWitt, D.L.; Smith, W.L.
Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence [published erratum appears in Proc Natl Acad Sci U S A 1988 Jul;85(14):5056]
Proc. Natl. Acad. Sci. USA
85
1412-1416
1988
Ovis aries
Narayanan, R.; Harrington, M.G.
Hydroperoxyeicosatetraenoic acid: an activator of prostaglandin synthase activity
Biochem. Soc. Trans.
8
449-450
1980
Ovis aries
Hemler, M.; Lands, W.E.M.
Purification of the cyclooxygenase that forms prostaglandins. Demonstration of two forms of iron in the holoenzyme
J. Biol. Chem.
251
5575-5579
1976
Ovis aries
Marnett, L.J.; Dix, T.A.; Sachs, R.J.; Siedlik, P.H.
Oxidations by fatty acid hydroperoxides and prostaglandin synthase
Adv. Prostaglandin Thromboxane Leukot. Res.
11
79-86
1983
Ovis aries
Egan, R.W.; Gale, P.O.H.; Baptista, E.M.; Kennicott, K.L.; VandenHeuvel, W.J.A.; Walker, R.W.; Fagerness, P.W.; Kuehl, F.A.
Oxidation reactions by prostaglandin cyclooxygenase-hydroperoxidase
J. Biol. Chem.
256
7352-7361
1981
Ovis aries
Van der Ouderaa, F.J.; Buytenhek, M.; Nugteren, D.H.; Van Dorp, D.A.
Acetylation of prostaglandin endoperoxide synthetase with acetylsalicylic acid
Eur. J. Biochem.
109
1-8
1980
Ovis aries
DeWitt, D.L.; Rollins, T.E.; Day, J.S.; Gauger, J.A.; Smith, W.L.
Orientation of the active site and antigenic determinants of prostaglandin endoperoxide synthase in the endoplasmic reticulum
J. Biol. Chem.
256
10375-10382
1981
Ovis aries
Roth, G.J.; Siok, C.J.; Ozols, J.
Structural characteristics of prostaglandin synthetase from sheep vesicular gland
J. Biol. Chem.
255
1301-1304
1980
Ovis aries
Marnett, L.J.; Chen, Y.N.P.; Maddipati, K.R.; Ple, P.; Labeque, R.
Functional differentiation of cyclooxygenase and peroxidase activities of prostaglandin synthase by trypsin treatment. Possible location of a prosthetic heme binding site
J. Biol. Chem.
263
16532-16535
1988
Ovis aries
Marnett, L.J.; Siedlick, P.H.; Ochs, R.C.; Pagels, W.R.; Das, M.; Honn, K.V.; Warnock, R.H.; Tainer, B.E.; Eling, T.E.
Mechanism of the stimulation of prostaglandin H synthase and prostacyclin synthase by the antithrombotic and antimetastatic agent, nafazatrom
Mol. Pharmacol.
26
328-335
1984
Ovis aries
Wlodawer, P.; Samuelsson, B.
On the organization and mechanism of prostaglandin synthetase
J. Biol. Chem.
248
5673-5678
1973
Ovis aries
Golly, I.; Hlavica, P.
N-Oxidation of 4-chloroaniline by prostaglandin synthase
Biochem. J.
260
803-809
1985
Ovis aries
Smith, C.J.; Marnett, L.J.
Effects of cysteine-to-serine mutations on structural and functional properties of prostaglandin endoperoxide synthase
Arch. Biochem. Biophys.
335
342-350
1996
Ovis aries
Mancini, J.A.; Riendeau, D.; Falgueyret, J.P.; Vickers, P.J.; O'Neill, G.P.
Arginine 120 of prostaglandin G/H synthase-1 is required for the inhibition by nonsteroidal anti-inflammatory drugs containing a carboxylic acid moiety
J. Biol. Chem.
270
29372-29377
1995
Ovis aries
Gupta, K.; Selinsky, B.S.; Kaub, C.J.; Katz, A.K.; Loll, P.J.
The 2.0 A resolution crystal structure of prostaglandin H2 synthase-1: structural insights into an unusual peroxidase
J. Mol. Biol.
335
503-518
2003
Ovis aries
Gupta, K.; Selinsky, B.S.; Loll, P.J.
2.0 A structure of prostaglandin H2 synthase-1 reconstituted with a manganese porphyrin cofactor
Acta crystallogr. Sect. D
62
151-156
2006
Ovis aries
Tsaplina, L.A.; Karatasso, Y.O.; Filimonov, I.S.; Vrzheshch, P.V.
Kinetic mechanism of the bifunctional enzyme prostaglandin-H-synthase. Effect of electron donors on the cyclooxygenase reaction
Biochemistry (Moscow)
71
1247-1255
2006
Ovis aries (P05979), Ovis aries (P79208)
Liu, W.; Cao, D.; Oh, S.F.; Serhan, C.N.; Kulmacz, R.J.
Divergent cyclooxygenase responses to fatty acid structure and peroxide level in fish and mammalian prostaglandin H synthases
FASEB J.
20
1097-1108
2006
Ovis aries, Homo sapiens (P23219), Homo sapiens (P35354), Salvelinus fontinalis (Q9PTN3), Salvelinus fontinalis (Q9PW89), Salvelinus fontinalis
Trostchansky, A.; Odonnell, V.B.; Goodwin, D.C.; Landino, L.M.; Marnett, L.J.; Radi, R.; Rubbo, H.
Interactions between nitric oxide and peroxynitrite during prostaglandin endoperoxide H synthase-1 catalysis: A free radical mechanism of inactivation
Free Radic. Biol. Med.
42
1029-1038
2007
Ovis aries
Deeb, R.S.; Hao, G.; Gross, S.S.; Laine, M.; Qiu, J.H.; Resnick, B.; Barbar, E.J.; Hajjar, D.P.; Upmacis, R.K.
Heme catalyzes tyrosine 385 nitration and inactivation of prostaglandin H2 synthase-1 by peroxynitrite
J. Lipid Res.
47
898-911
2006
Ovis aries
Reininger, E.A.; Bauer, R.
Prostaglandin-H-synthase (PGHS)-1 and -2 microtiter assays for the testing of herbal drugs and in vitro inhibition of PGHS-isoenzyms by polyunsaturated fatty acids from Platycodi radix
Phytomedicine
13
164-169
2006
Ovis aries (P05979), Ovis aries (P79208)
Gersting, J.; Schaub, C.E.; Keller-Wood, M.; Wood, C.E.
Inhibition of brain prostaglandin endoperoxide synthase-2 prevents the preparturient increase in fetal adrenocorticotropin secretion in the sheep fetus
Endocrinology
149
4128-4136
2008
Ovis aries (P05979), Ovis aries (P79208), Ovis aries
Tsai, A.L.; Kulmacz, R.J.
Prostaglandin H synthase: Resolved and unresolved mechanistic issues
Arch. Biochem. Biophys.
493
103-124
2010
Ovis aries (P05979), Homo sapiens (P23219), Homo sapiens (P35354)
Goncalves, L.L.; Ramkissoon, A.; Wells, P.G.
Prostaglandin H synthase-1-catalyzed bioactivation of neurotransmitters, their precursors, and metabolites: oxidative DNA damage and electron spin resonance spectroscopy studies
Chem. Res. Toxicol.
22
842-852
2009
Ovis aries (P05979)
Gersting, J.A.; Schaub, C.E.; Wood, C.E.
Development of prostaglandin endoperoxide synthase expression in the ovine fetal central nervous system and pituitary
Gene Expr. Patterns
9
603-611
2009
Ovis aries (P05979), Ovis aries (P79208), Ovis aries
Kawakami, Y.; Nakamura, T.; Hosokawa, T.; Suzuki-Yamamoto, T.; Yamashita, H.; Kimoto, M.; Tsuji, H.; Yoshida, H.; Hada, T.; Takahashi, Y.
Antiproliferative activity of guava leaf extract via inhibition of prostaglandin endoperoxide H synthase isoforms
Prostaglandins Leukot. Essent. Fatty Acids
80
239-245
2009
Ovis aries (P05979), Ovis aries (P79208), Homo sapiens (P23219), Homo sapiens (P35354), Homo sapiens
Bedi, G.; Tonzibo, Z.; Oussou, K.; Choppard, C.; Mahy, J.; NGuessan, T.
Effect of essential oil of chromoleana odorata (asteraceae) from ivory coast, on cyclooxygenase function of prostagladin-H synthase activity
Afr. J. Pharm. Pharmacol.
4
535-538
2010
Ovis aries
-
Trushkin, N.A.; Filimonov, I.S.; Vrzheshch, P.V.
Inhibition of cyclooxygenase activity of prostaglandin-H-synthase by excess substrate (molecular oxygen)
Biochemistry (Moscow)
75
1368-1373
2010
Ovis aries
Gupta, K.; Selinsky, B.S.
Bacterial and algal orthologs of prostaglandin H2 synthase novel insights into the evolution of an integral membrane protein
Biochim. Biophys. Acta
1848
83-94
2015
Ovis aries (P05979)
El-Nagar, M.K.S.; Abdu-Allah, H.H.M.; Salem, O.I.A.; Kafafy, A.N.; Farghaly, H.S.M.
Novel N-substituted 5-aminosalicylamides as dual inhibitors of cyclooxygenase and 5-lipoxygenase enzymes Synthesis, biological evaluation and docking study
Bioorg. Chem.
78
80-93
2018
Ovis aries, Homo sapiens
EXTERNAL LINKS (specific for EC-Number 1.14.99.1)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
