Information on EC 1.14.19.B6 - tryptophan 6-halogenase

for references in articles please use BRENDA:EC1.14.19.B6
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The expected taxonomic range for this enzyme is: Streptomyces toxytricini

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EC NUMBER
COMMENTARY hide
1.14.19.B6
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
tryptophan 6-halogenase
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-tryptophan + FADH2 + Cl- + O2 + H+ = 6-chloro-D-tryptophan + FAD + 2 H2O
show the reaction diagram
L-tryptophan + FADH2 + Cl- + O2 + H+ = 6-chloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
tryptophan:FADH2 oxidoreductase (6-halogenating)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-tryptophan + FADH2 + Cl- + O2 + H+
6-chloro-D-tryptophan + FAD + 2 H2O
show the reaction diagram
-
-
-
?
L-tryptophan + FADH2 + Br- + O2 + H+
6-bromo-L-tryptophan + FAD + 2 H2O
show the reaction diagram
-
-
-
?
L-tryptophan + FADH2 + Cl- + O2 + H+
6-chloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
-
-
-
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
L-tryptophan
pH 6.0, 28°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0255
L-tryptophan
pH 6.0, 28°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
L-tryptophan
pH 6.0, 28°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
pH 5.0: about 75% of maximal activity, pH 7.0: about 70% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
35°C: about 75% of maximal activity, 45°C: about 75% of maximal activity
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
x * 59000, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 59000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His6-tagged enzyme is expressed in Escherichia coli RIL
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.19.B6)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)