Information on EC 1.14.19.B5 - 7-chloro-L-tryptophan 6-halogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)
Please login to have access to the AMENDA and FRENDA data


The expected taxonomic range for this enzyme is: Kutzneria sp. 744

top print hide
EC NUMBER
COMMENTARY hide
1.14.19.B5
preliminary BRENDA-supplied EC number
top print hide
RECOMMENDED NAME
GeneOntology No.
7-chloro-L-tryptophan 6-halogenase
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+ = 6,7-dichloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
-
-
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
7-chloro-L-tryptophan:FADH2 oxidoreductase (6-halogenating)
-
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
top print hide
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides)
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+
6,7-dichloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
L-tryptophan + FADH2 + Cl- + O2 + H+
6-chloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
kcat/Km for the halogenation of L-tryptophan is less than 1% compared to the value for the halogenation 7-chloro-L-tryptophan
-
-
?
additional information
?
-
KtzR is not active for introduction of chloride to piperazic acid or gamma,delta-dehydropiperazic acid
-
-
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+
6,7-dichloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
A8CF74
the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides)
-
-
?
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.114
7-chloro-L-tryptophan
pH 7.0, 22°C
0.808
L-tryptophan
pH 7.0, 22°C
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
7-chloro-L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22°C
0.0013
L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22°C
top print hide
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
7-chloro-L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22°C
11727
0.0016
L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22°C
119
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.19.B5)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)