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Enzyme Nomenclature
Information on EC 1.14.19.48 - tert-amyl alcohol desaturase
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
1.14.19.48
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RECOMMENDED NAME
GeneOntology No.
tert-amyl alcohol desaturase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
tert-amyl alcohol + NADPH + H+ + O2 = isoprenyl alcohol + NADP+ + 2 H2O
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SYSTEMATIC NAME
IUBMB Comments
tert-amyl alcohol,NADPH:oxygen oxidoreductase (1,2-dehydrogenating)
The enzyme, characterized from the bacterium Aquincola tertiaricarbonis, is a Rieske nonheme mononuclear iron oxygenase. It can also act, with lower efficiency, on butan-2-ol, converting it to but-1-en-3-ol. Depending on the substrate, the enzyme also catalyses EC 1.14.13.229, tert-butanol monooxygenase.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
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degradation of tert-amyl alcohol leads to formation of beta and gamma isomers of isoamylene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increased with decreasing oxygen concentrations
physiological function
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degradation of tert-amyl alcohol leads to formation of beta and gamma isomers of isoamylene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increased with decreasing oxygen concentrations
physiological function
degradation of tert-amyl alcohol leads to formation of beta and gamma isomers of isoamylene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increased with decreasing oxygen concentrations
physiological function
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MdpJ knockout mutants are not able to grow on the tertiary alcohols tert-butanol and tert-amyl alcohol, whereas 2-methylpropan-1,2-diol, the putative product of tert-butanol hydroxylation by MdpJ catalysis can still be used as the sole source of energy and carbon. The postulated 2-methylpropan-1,2-diol intermediate 2-hydroxyisobutanoate is metabolized by wild-type and mutant strains at the same rates. tert-amyl alcohol and hemiterpene degradation are linked with the catabolic route of leucine
physiological function
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degradation of tert-amyl alcohol leads to formation of beta and gamma isomers of isoamylene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increased with decreasing oxygen concentrations; MdpJ knockout mutants are not able to grow on the tertiary alcohols tert-butanol and tert-amyl alcohol, whereas 2-methylpropan-1,2-diol, the putative product of tert-butanol hydroxylation by MdpJ catalysis can still be used as the sole source of energy and carbon. The postulated 2-methylpropan-1,2-diol intermediate 2-hydroxyisobutanoate is metabolized by wild-type and mutant strains at the same rates. tert-amyl alcohol and hemiterpene degradation are linked with the catabolic route of leucine
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-methyl-3-pentanol + NADPH + H+ + O2
3-methyl-1-penten-3-ol + NADP+ + 2 H2O
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?
3-methyl-3-pentanol + NADPH + H+ + O2
3-methyl-1-penten-3-ol + NADP+ + 2 H2O
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?
tert-amyl alcohol + NADPH + H+ + O2
isoprenyl alcohol + NADP+ + 2 H2O
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?
tert-amyl alcohol + NADPH + H+ + O2
isoprenyl alcohol + NADP+ + 2 H2O
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?
tert-amyl alcohol + NADPH + H+ + O2
isoprenyl alcohol + NADP+ + 2 H2O
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?
tert-amyl alcohol + NADPH + H+ + O2
isoprenyl alcohol + NADP+ + 2 H2O
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?
tert-amyl alcohol + NADPH + H+ + O2
isoprenyl alcohol + NADP+ + 2 H2O
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?
tert-amyl alcohol + NADPH + H+ + O2
isoprenyl alcohol + NADP+ + 2 H2O
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?
additional information
?
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MdpJ is not hydroxylating tert-amyl alcohol to diols but functions as a desaturase, resulting in the formation of the hemiterpene 3-methyl-1-buten-3-ol. The latter is further processed via the hemiterpenes prenol, prenal, and 3-methylcrotonic acid
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.13.229, tert-butyl alcohol monooxygenase
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.13.229, tert-butyl alcohol monooxygenase
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additional information
?
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MdpJ is not hydroxylating tert-amyl alcohol to diols but functions as a desaturase, resulting in the formation of the hemiterpene 3-methyl-1-buten-3-ol. The latter is further processed via the hemiterpenes prenol, prenal, and 3-methylcrotonic acid
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.13.229, tert-butyl alcohol monooxygenase
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.13.229, tert-butyl alcohol monooxygenase
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