Information on EC 1.14.19.3 - acyl-CoA 6-desaturase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.14.19.3
-
RECOMMENDED NAME
GeneOntology No.
acyl-CoA 6-desaturase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O
show the reaction diagram
(2)
-
-
-
linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = gamma-linolenoyl-CoA + 2 ferricytochrome b5+ 2 H2O
show the reaction diagram
(1)
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
docosahexaenoate biosynthesis III (mammals)
-
-
gamma-linolenate biosynthesis II (animals)
-
-
icosapentaenoate biosynthesis II (metazoa)
-
-
Linoleic acid metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
linoleoyl-CoA,hydrogen-donor:oxygen oxidoreductase
An iron protein. The rat liver enzyme is an enzyme system involving cytochrome b5 and EC 1.6.2.2, cytochrome-b5 reductase.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
acyl-CoA DELTA6-desaturase
-
-
D6Des
Muraenesox cinereus pike eel
-
-
DEALTA6I
-
-
DELTA 6-desaturase
-
-
DELTA 6-desaturase
-
DELTA 6-desaturase
Muraenesox cinereus pike eel
-
-
delta 6-fatty acid desaturase
-
-
delta 6-fatty acid desaturase
-
delta 6-fatty acid desaturase
Muraenesox cinereus pike eel
-
-
delta 6-fatty acid desaturase
-
delta 6-fatty acid desaturase
Thamnidium elegans As3.2806
-
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
DELTA-6 desaturase
-
delta-6 fatty acid desaturase
-
delta-6 fatty acid desaturase
Cunninghamella echinulata MIAN6
-
-
delta-6 fatty acid desaturase
-
DELTA-6-desaturase
-
-
DELTA6 desaturase
-
-
DELTA6 desaturase
-
-
DELTA6 desaturase
-
-
DELTA6 desaturase
-
-
DELTA6 desaturase
-
-
DELTA6 desaturase
-
DELTA6 fatty acid desaturase
-
DELTA6 fatty acid desaturase
-
DELTA6 fatty acid desaturase
-
-
DELTA6 fatty acid desaturase
-
DELTA6 fatty acid desaturase
-
-
DELTA6 fatty acid desaturase
Tetrahymena thermophila ATCC30007
-
-
-
DELTA6 fatty acyl desaturases
-
DELTA6-acyl CoA desaturase
-
-
-
-
DELTA6-desaturase
-
-
-
-
DELTA6-desaturase
-
DELTA6-desaturase
-
-
DELTA6-desaturase
-
DELTA6-desaturase
-
-
DELTA6-desaturase
-
DELTA6-desaturase II
-
DELTA6-fatty acid desaturase
-
-
DELTA6-fatty acid desaturase
-
-
DELTA6-fatty acid desaturase
Rhizopus stolonifer R31.6
-
-
-
DELTA6-fatty acid desaturase
-
-
DELTA6-fatty acyl-CoA desaturase
-
-
-
-
DELTA6fad_b
gene name
DES6
Tetrahymena thermophila ATCC30007
-
-
-
desaturase, fatty acid DA6-
-
-
-
-
desaturase, linoleate
-
-
-
-
EC 1.14.99.25
-
-
formerly
-
FADS2
-
-
fatty acid 6-desaturase
-
-
-
-
fatty acid DA6-desaturase
-
-
-
-
fatty acid DELTA 6-desaturase
-
-
fatty acid DELTA 6-desaturase
-
fatty acid DELTA 6-desaturase
-
fatty acid delta-6 desaturase
-
-
fatty acid DELTA6-desaturase
-
fatty acid desaturase-2
-
-
linoleate desaturase
-
-
-
-
linoleic acid desaturase
-
-
-
-
linoleic desaturase
-
-
-
-
linoleoyl CoA desaturase
-
-
-
-
linoleoyl-coenzyme A desaturase
-
-
-
-
long-chain fatty acid DELTA6-desaturase
-
-
-
-
PiDesD6
-
gene name
CAS REGISTRY NUMBER
COMMENTARY
9014-34-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Arabidopsis thaliana Heynh.
Heynh
-
-
Manually annotated by BRENDA team
strain C1, gene desD
UniProt
Manually annotated by BRENDA team
strain C1, gene desD
UniProt
Manually annotated by BRENDA team
common borage
-
-
Manually annotated by BRENDA team
strain MIAN6
UniProt
Manually annotated by BRENDA team
Cunninghamella echinulata MIAN6
strain MIAN6
UniProt
Manually annotated by BRENDA team
DELTA6-desaturase D6D-V; contains several DELTA6-desaturase splicing variants
UniProt
Manually annotated by BRENDA team
DELTA6-desaturase D6D; contains several DELTA6-desaturase splicing variants
UniProt
Manually annotated by BRENDA team
soybean, cv Tracy
-
-
Manually annotated by BRENDA team
gene FADS1
-
-
Manually annotated by BRENDA team
linseed, cultivar Punjab
-
-
Manually annotated by BRENDA team
gene PiDesD6
-
-
Manually annotated by BRENDA team
1S-4, contains two functional DELTA6-desaturase genes for DELTA6-desaturase II and DELTA6-desaturase I
SwissProt
Manually annotated by BRENDA team
1S-4, two functional DELTA6 desaturrases, DELTA6I and DELTA6II exist in wild-type strain. DELTA6 desaturase-defective mutants strains YB214, HR95 and ST66
-
-
Manually annotated by BRENDA team
Muraenesox cinereus pike eel
-
UniProt
Manually annotated by BRENDA team
gene ObD6Des
UniProt
Manually annotated by BRENDA team
; DELTA6-desaturase
SwissProt
Manually annotated by BRENDA team
enzyme expressed in COS cells
-
-
Manually annotated by BRENDA team
INS-1 beta-cells
-
-
Manually annotated by BRENDA team
strain Sprague-Dawley
-
-
Manually annotated by BRENDA team
strain Wistar
-
-
Manually annotated by BRENDA team
Wistar BB/E n=12, Wistar BB/E n=6
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
strain Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
strain Wistar
-
-
Manually annotated by BRENDA team
synonym Rhizopus stolonifer
UniProt
Manually annotated by BRENDA team
Rhizopus stolonifer R31.6
-
-
-
Manually annotated by BRENDA team
gene DELTA6fad_a; gene DELTA6fad_a
SwissProt
Manually annotated by BRENDA team
gene DELTA6fad_b; gene DELTA6fad_b
UniProt
Manually annotated by BRENDA team
gene DELTA6fad_c; gene DELTA6fad_c
UniProt
Manually annotated by BRENDA team
Tetrahymena thermophila ATCC30007
-
-
-
Manually annotated by BRENDA team
strain As.3.2806
-
-
Manually annotated by BRENDA team
strain As3.2806
UniProt
Manually annotated by BRENDA team
Thamnidium elegans As.3.2806
strain As.3.2806
-
-
Manually annotated by BRENDA team
Thamnidium elegans As3.2806
strain As3.2806
UniProt
Manually annotated by BRENDA team
black-eyed Susan vine
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
the enzyme takes part in the fatty Acid synthesis pathway, detailed overview
metabolism
delta 6-desaturase involved in very-long-chain polyunsaturated fatty acid synthesis
physiological function
-
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
-
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
-
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
-
mechanisms involved in the nutritional modulation of DELTA6D, overview
physiological function
fatty acid DELTA6-desaturase is the essential enzyme for polyunsaturated fatty acids biosynthesis and is responsible for the first step by converting linoleic acid to gamma-linolenoyl-CoA, and alpha-linolenic acid to stearidonic acid
metabolism
Muraenesox cinereus pike eel
-
delta 6-desaturase involved in very-long-chain polyunsaturated fatty acid synthesis
-
additional information
-
comparison of seawater and freshwater fish desaturated fatty acid contents, expression profiles and substrate specificities, overview
additional information
comparison of seawater and freshwater fish desaturated fatty acid contents, expression profiles and substrate specificities, overview
additional information
comparison of seawater and freshwater fish desaturated fatty acid contents, expression profiles and substrate specificities, overview
additional information
comparison of seawater and freshwater fish desaturated fatty acid contents, expression profiles and substrate specificities, overview
additional information
-
comparison of seawater and freshwater fish desaturated fatty acid comtents, expression profiles and substrate specificities, overview
additional information
comparison of seawater and freshwater fish desaturated fatty acid contents, expression profiles and substrate specificities, overview
additional information
-
comparison of seawater and freshwater fish desaturated fatty acid comtents, expression profiles and substrate specificities, overview
additional information
comparison of seawater and freshwater fish desaturated fatty acid contents, expression profiles and substrate specificities, overview
additional information
-
comparison of seawater and freshwater fish desaturated fatty acid contents, expression profiles and substrate specificities, overview
additional information
nutritional regulation, overview; nutritional regulation, overview; nutritional regulation, overview
additional information
-
fat-3 mutant phenotype, lifespan, and transgenerational effect of cis- and trans-triglyceride diets, overview
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
24-carbon fatty acid + AH2 + O2
? + A + H2O
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
-
conversion rate is 19%
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
conversion rates of 45.9%
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
alpha-linolenic acid + AH2 + O2
stearidonic acid + A + H2O
show the reaction diagram
Muraenesox cinereus pike eel
conversion rates of 45.9%
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
-
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids, conversion rate: 23.1
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
conversion rate: 31.5
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
-
conversion rate: 59.5
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
conversion rate: 7.0
-
?
alpha-linolenic acid + AH2 + O2
?
show the reaction diagram
-
-
-
?
alpha-linolenic acid + AH2 + O2
?
show the reaction diagram
preferred substrate
-
-
?
alpha-linolenic acid + AH2 + O2
octadeca-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
-
-
?
alpha-linolenic acid + AH2 + O2
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
preferred substrate
-
?
alpha-linolenic acid + AH2 + O2
octadecatetraenoic acid + A + H2O
show the reaction diagram
-
-
-
?
alpha-linoleoyl-CoA + AH2 + O2
stearidonoyl-CoA + A + 2 H2O
show the reaction diagram
-
-
-
?
alpha-linoleoyl-CoA + AH2 + O2
stearidonoyl-CoA + A + 2 H2O
show the reaction diagram
-
-
?
gamma-linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
39% conversion efficiency
-
?
gamma-linolenic acid + AH2 + O2
cis-6,9,12,15-octadecatetraenoic acid + A + H2O
show the reaction diagram
-
-
-
?
gamma-linolenic acid methyl ester + O2
?
show the reaction diagram
-
-
-
-
?
gamma-linolenic acid methyl ester + O2
?
show the reaction diagram
-
7% conversion efficiency
-
-
?
gamma-linolenic acid methyl ester + O2
?
show the reaction diagram
Rhizopus stolonifer R31.6
-
-
-
-
?
heptadec-9-enoic acid + AH2 + O2
heptadec-6,9-dienoic acid + A + H2O
show the reaction diagram
-
conversion rate is 17%
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
key enzyme localized in the endoplasmic reticulum
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
important for the generation of unsaturated fatty acids, DELTA6-desaturase required for the conversion of dietary linoleic acid to arachidonic acid
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
DELTA6-desaturase reaction is the rate-limiting step in the conversion of linoleic acid and alpha linoleic acids to the longer, more highly unsaturated members of the n-6 and n-3 polyunsaturated fatty acids, metabolic pathway in mammalian cells
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
conversion rate: 1.5
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
conversion rate: 12.2
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
conversion rate: 3.6
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
conversion rate: 31.2
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
14.4% conversion rate
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
conversion rates of 14.2%
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Muraenesox cinereus pike eel
conversion rates of 14.2%
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Thamnidium elegans As.3.2806
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Cunninghamella echinulata MIAN6
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rhizopus stolonifer R31.6
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Tetrahymena thermophila ATCC30007
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Arabidopsis thaliana Heynh.
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
?
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
?
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linoleic acid + A + H2O
show the reaction diagram
-
-
-
?
linolenic acid + AH2 + O2
arachidonic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleoyl phosphatidylcholine + AH2 + O2
gamma-linolenoylphosphatidylcholine + A + H2O
show the reaction diagram
-
-
-
?
linoleoyl-CoA + AH2 + O2
gamma-linolenoyl-CoA + A + 2 H2O
show the reaction diagram
-
-
-
-
linoleoyl-CoA + AH2 + O2
gamma-linolenoyl-CoA + A + 2 H2O
show the reaction diagram
-
-
-
?
linoleoyl-CoA + AH2 + O2
gamma-linolenoyl-CoA + A + 2 H2O
show the reaction diagram
-
-
?
linoleoyl-CoA + AH2 + O2
gamma-linolenoyl-CoA + A + 2 H2O
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
octadec-9,12-dienoic acid + AH2 + O2
octadec-6,9,12-trienoic acid + A + H2O
show the reaction diagram
-
conversion rate is 11.5%
-
?
octadec-9-enoic acid + AH2 + O2
octadec-6,9-dienoic acid + A + H2O
show the reaction diagram
-
conversion rate is 19.1%
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
octadeca-9,12-dienoic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Arabidopsis thaliana Heynh.
-
-
-
?
octadecanoate + AH2 + O2
? + A + H2O
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
-
-
?
oleic acid + AH2 + O2
?
show the reaction diagram
-
-
-
-
?
palmitate + AH2 + O2
sapienate + A + H2O
show the reaction diagram
the enzyme is a component of the lipid metabolic pathway that converts the essential fatty acids linoleate and alpha-linolenate into long-chain polyunsaturated fatty acids. The DELTA-6 desaturase/FADS2 expressed in human sebocytes catalyzes the conversion of palmitate into sapienate
-
?
palmitic acid + AH2 + O2
hexadec-6-enoic acid + A + H2O
show the reaction diagram
-
key enzyme required for numerous vital functions involving distinct polyunsaturated fatty acids and polyunsaturated fatty acid-derived bioactive lipids. It seems that the biological importance of DELTA6-desaturase activity should also be considered for its newly identified role in the control of the biosynthesis of a monoenoic fatty acid (C16:1 n-10), particularly in tissues with low DELTA9-desaturase activity
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
6-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
6-octadecenoyl-[acyl-carrier protein] + 9-octadecenoyl-[acyl-carrier protein]acceptor + H2O
show the reaction diagram
-
-
ratio of activity: 2/1
?
tetracosapentaenoic acid + AH2 + O2
tetracosahexaenoic acid + A + H2O
show the reaction diagram
-
-
-
?
monogalactosydiacylglycerol + AH2 + O2
? + A + H2O
show the reaction diagram
Arabidopsis thaliana, Arabidopsis thaliana Heynh.
-
-
-
?
additional information
?
-
fatty acid DELTA6-desaturation is the first commited step in C20 polyunsaturated fatty acid biosynthesis
-
-
-
additional information
?
-
fatty acid DELTA6-desaturation is the first commited step in C20 polyunsaturated fatty acid biosynthesis
-
-
-
additional information
?
-
-
low activity with C15:1DELTA9 fatty acid and C16:1DELTA9 fatty acid
-
-
-
additional information
?
-
no activity with linoleic acid
-
-
-
additional information
?
-
-
the enzyme is essential for the formation of long-chain metabolites from dietary linoleic acid and alpha-linolenic acid, but shows a slow activity in humans. A defect in the activity of DELTA6 and DELTA5 desaturases decreases the formation of gamma-linolenic acid, dihomo-gamma-linoleic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid from dietary linoleic acid, and alpha-linolenic acid. This, in turn, leads to inadequate formation of prostaglandins E1 and I3, prostacyclin, lipoxins resolvins, neuroprotectin D1, NO, and nitrolipids that have anti-inflammatory and platelet anti-aggregatory actions, inhibit leukocyte activation, augment wound healing, and resolve inflammation and thus, leads to the initiation and progression of atheroslcerosis, metabolism of essential fatty acids, overview, enzyme activity is decreased in diabetes mellitus, hypertension, hyperlipidemia, and metabolic syndrome X, alpha-linoleic acid is converted to eicosapentaenoic acid by the enzyme, the enzyme is also active with linoleic, which is converted to gamma-linoleic acid, and oleic acid
-
-
-
additional information
?
-
-
PiDes6 desaturates both omegax6 and omega3 substrates, substrate specificity in comparison to other fatty acid desaturases, overview
-
-
-
additional information
?
-
the zebrafish enzyme is a bifunctional DELTA5/DELTA6 desaturase
-
-
-
additional information
?
-
no activity with 20:4n-3, 20:3n-6, 22:5n-3, and 22:4n-6 with by DELTA6fad_b
-
-
-
additional information
?
-
no activity with 20:4n-3, 20:3n-6, 22:5n-3, and 22:4n-6 with DELTA6fad_c
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
-
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
Q9DEX6
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
Q98SW7
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
alpha-linolenic acid + AH2 + O2
octadec-6,9,12,15-tetraenoic acid + A + H2O
show the reaction diagram
Q8AY64
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
alpha-linoleoyl-CoA + AH2 + O2
stearidonoyl-CoA + A + 2 H2O
show the reaction diagram
E1U2Q3
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
key enzyme localized in the endoplasmic reticulum
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
important for the generation of unsaturated fatty acids, DELTA6-desaturase required for the conversion of dietary linoleic acid to arachidonic acid
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
DELTA6-desaturase reaction is the rate-limiting step in the conversion of linoleic acid and alpha linoleic acids to the longer, more highly unsaturated members of the n-6 and n-3 polyunsaturated fatty acids, metabolic pathway in mammalian cells
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
-
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Q9DEX6
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Q98SW7
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Q8AY64
the enzyme is responsible for the first and rate-limiting step in biosynthesis of highly unsaturated fatty acids
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
linoleic acid + AH2 + O2
gamma-linolenic acid + A + H2O
show the reaction diagram
Arabidopsis thaliana Heynh.
-
-
-
?
palmitate + AH2 + O2
sapienate + A + H2O
show the reaction diagram
O95864
the enzyme is a component of the lipid metabolic pathway that converts the essential fatty acids linoleate and alpha-linolenate into long-chain polyunsaturated fatty acids. The DELTA-6 desaturase/FADS2 expressed in human sebocytes catalyzes the conversion of palmitate into sapienate
-
?
palmitic acid + AH2 + O2
hexadec-6-enoic acid + A + H2O
show the reaction diagram
-
key enzyme required for numerous vital functions involving distinct polyunsaturated fatty acids and polyunsaturated fatty acid-derived bioactive lipids. It seems that the biological importance of DELTA6-desaturase activity should also be considered for its newly identified role in the control of the biosynthesis of a monoenoic fatty acid (C16:1 n-10), particularly in tissues with low DELTA9-desaturase activity
-
?
linoleoyl-CoA + AH2 + O2
gamma-linolenoyl-CoA + A + 2 H2O
show the reaction diagram
E1U2Q3
-
-
?
additional information
?
-
Q84KG8
fatty acid DELTA6-desaturation is the first commited step in C20 polyunsaturated fatty acid biosynthesis
-
-
-
additional information
?
-
Q84KG6
fatty acid DELTA6-desaturation is the first commited step in C20 polyunsaturated fatty acid biosynthesis
-
-
-
additional information
?
-
-
the enzyme is essential for the formation of long-chain metabolites from dietary linoleic acid and alpha-linolenic acid, but shows a slow activity in humans. A defect in the activity of DELTA6 and DELTA5 desaturases decreases the formation of gamma-linolenic acid, dihomo-gamma-linoleic acid, arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid from dietary linoleic acid, and alpha-linolenic acid. This, in turn, leads to inadequate formation of prostaglandins E1 and I3, prostacyclin, lipoxins resolvins, neuroprotectin D1, NO, and nitrolipids that have anti-inflammatory and platelet anti-aggregatory actions, inhibit leukocyte activation, augment wound healing, and resolve inflammation and thus, leads to the initiation and progression of atheroslcerosis, metabolism of essential fatty acids, overview, enzyme activity is decreased in diabetes mellitus, hypertension, hyperlipidemia, and metabolic syndrome X
-
-
-
additional information
?
-
-
PiDes6 desaturates both omegax6 and omega3 substrates, substrate specificity in comparison to other fatty acid desaturases, overview
-
-
-
additional information
?
-
Q9DEX7
the zebrafish enzyme is a bifunctional DELTA5/DELTA6 desaturase
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cytochrome b5
-
absolutely essential for DELTA6-desaturation
-
cytochrome b5
-
with NADPH
-
cytochrome b5
contains a cytochrome b5 heme-binding domain
-
cytochrome b5
binds at the N-terminus of the enzyme
-
cytochrome b5
-
required for activity
-
Ferredoxin
-
-
-
Ferredoxin
-
highest activity with Anabaena ferredoxin
-
Ferredoxin
-
with FADH2
-
heme
contains a cytochrome b5 heme-binding domain
linoleoyl-CoA
-
absolutely essential for DELTA6-desaturation
NADH
-
absolutely essential for DELTA6-desaturation
NADPH
-
absolutely essential for DELTA6-desaturation
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
-
DELTA6-desaturase contains 15.1 nmol iron/mg protein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4-chloro-5-dimethylamino-2-phenyl-3(2H)-pyridazinone
-
BASF 13-338, San 9785, herbicide known to reduce levels of linolenic acid, inhibits in vitro desaturation of fatty acids
bathophenanthroline sulfonate
-
mild inhibition
beta-mercaptoethanol
-
mild inhibition
cholesterol
-
-
conjugated linoleic acid
-
all CLA isomers inhibits significantly the activity of DELTA6-desaturase, the c9,t11 isomer is the most potent inhibitor
-
dithiothreitol
-
mild inhibition
Iron chelators
-
-
-
N-ethylmaleimide
-
only little effect
N-ethylmaleimide
-
n-3 DELTA6-desaturase is not inhibited, it increases, NEM specifically inhibits DELTA6-desaturase activity in the n-6 series, but not in the n-3-series
N-ethylmaleimide
-
-
p-chloromercuribenzene sulfonate
-
-
saturated fatty acids
-
-
-
trans-fats
-
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
bovine serum albumin
-
1-10 mg/ml, stimulates enzyme activity of unwashed microsomes by 50%
-
catalase
-
bovine catalase
-
catalase
-
EC 1.11.1.6
-
catalase
-
activity stabilized by addition
-
NADH-cytochrome b5 reductase
-
EC 1.6.2.2, absolutely essential for DELTA6-desaturation
-
NADPH-cytochrome b5 reductase
-
EC 1.6.2.2, absolutely essential for DELTA6-desaturation
-
O2
-
absolutely essential for DELTA6-desaturation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.045
linoleoyl-CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.1
-
substrate palmitoyl-[acyl-carrier protein]
additional information
-
0.3 mM exogenously added NADPH to Escherichia coli cells co-expressing DELTA6 desaturase and cytochrome b5 leads to an increase of the level of gamma-linoleic acid product by 50%, but no effect in Escherichia coli co-expressing DELTA6 desaturase and ferredoxin under the same conditions; co-addition of NADPH and FAD to Escherichia coli cells co-expressing DELTA6 desaturase and ferredoxin leads to an increase of the level of gamma-linoleic acid product; no activity when expressed in Escherichia coli alone, co-expression of cytochrome b5 is needed
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.9
-
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
expression is higher in salmon fed a diet containing vegetable oil than in fish fed a diet containing fish oil
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
skin fibroblast
Manually annotated by BRENDA team
-
much lower activity than in Sertoli cells
Manually annotated by BRENDA team
-
treatment with myristic acid specifically increases DELTA6-desaturase activity
Manually annotated by BRENDA team
-
high expression, expression is higher in salmon fed a diet containing vegetable oil than in fish fed a diet containing fish oil
Manually annotated by BRENDA team
Arabidopsis thaliana Heynh.
-
-
-
Manually annotated by BRENDA team
-
fetal liver
Manually annotated by BRENDA team
-
high expression, expression is higher in salmon fed a diet containing vegetable oil than in fish fed a diet containing fish oil
Manually annotated by BRENDA team
Muraenesox cinereus pike eel, Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
red muscle: expression is higher in salmon fed a diet containing vegetable oil than in fish fed a diet containing fish oil
Manually annotated by BRENDA team
Thamnidium elegans As3.2806
-
-
-
Manually annotated by BRENDA team
-
mRNA level is upregulated by dexamethasone, follicle-stimulating hormone or cAMP
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
;
-
Manually annotated by BRENDA team
additional information
-
wide tissue distribution
Manually annotated by BRENDA team
additional information
tissue distribution profile, overview; tissue distribution profile, overview; tissue distribution profile, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Arabidopsis thaliana Heynh.
-
-
-
Manually annotated by BRENDA team
-
membrane-bound
-
Manually annotated by BRENDA team
-
membrane-bound, PiDes6 contains a fused cytochrome b5 at its N-terminus, supporting its microsomal localization, similar to other functionally characterized front-end desaturases
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
membrane; membrane
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45000
-
recombinant DELTA6-desaturase expressed in COS-7 cells, analysed by Western blot
438422
47000
-
Western Blot, detection of histidine-tagged DELTA6 desaturase expressed in Escherichia coli using a monoclonal antibody against the histidine-tag
675898
52000
calculated from amino acid sequence
699496
52410
calculated from amino acid sequence
700115
65000 - 68000
-
gel filtration
438414
66000
-
SDS-PAGE
438414
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 52410, calculated from amino acid sequence
?
-
x * 51800, calculated from amino acid sequence
?
Rhizopus stolonifer R31.6
-
x * 52410, calculated from amino acid sequence
-
monomer
-
1 * 66000, SDS-PAGE
monomer
Rattus norvegicus Wistar
-
1 * 66000, SDS-PAGE
-
additional information
the enzyme structure shows three conserved histidine-rich motifs and two hydrophobic domains, a cytochrome b5-like domain is observed at the N-terminus
additional information
the enzyme sequence comprises the characteristics of membrane-bound desaturases, including three conserved histidine-rich motifs, hydropathy profile, and a cytochrome b5 domain at the N-terminus, overview
additional information
Thamnidium elegans As3.2806
-
the enzyme structure shows three conserved histidine-rich motifs and two hydrophobic domains, a cytochrome b5-like domain is observed at the N-terminus
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospholipoprotein
-
20-40 M phospholipid per mol of protein
phospholipoprotein
Rattus norvegicus Wistar
-
20-40 M phospholipid per mol of protein
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, stored under nitrogen the enzyme is unstable, loses 80% of its activity after repeated freezing and thawing
-
15°C, full-length enzyme in 100 mM sodium phosphate, pH 7.0, 4 h, 16% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional expression in Saccharomyces cerevisiae strain INVSc1
-
expressed in Saccharomyces cerevisiae strain InvSc1
-
expression in Saccharomyces cerevisiae
-
expressed in Escherichia coli DH5alpha, co-expressed with cytochrome b5 and ferredoxin, no enzyme activity when expressed in Escherichia coli alone
-
expressed in Saccharomyces cerevisiae, strain DBY746 with and without supplementation with linoleic acid
-
gene desD, functional analysis of the Spirulina promoter in Escherichia coli, the -10 sequence, TATAAT, located at -33 bp relative to the translation start site, is essential for D6D promoter function, an ‘AT-rich inverted repeat’ -192 to -164 serves as a target-binding site for a transcriptional regulator
expressed in HEK-293 cells
transformed into Agrobacterium tumefaciens and expressed in Glycine max
-
gene fat-3, quantitative real-time PCR expression analysis, overview
-
expressed in Pichia pastoris strain GS115
expression in Saccharomyces cerevisaiae
-
D6D, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis; D6D-V, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
expressed in Saccharomyces cerevisiae strain BY4741, two different mRNA variants
-
DELTA6-desaturase gene identified in human chromosome 11
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human sequence determined
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gene PiDesD6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Saccharomyces cerevisiae strain W303, real-time quantitative PCR expression analysis
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DELTA6-desaturase II cDNA expressed in Aspergillus oryzae
Saccharomyces cerevisiae transformed with fungal DELTA6-desaturase gene
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expressed in Saccharomyces cerevisiae strain InvSc1
gene ObD6Des, DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression in Saccharomyces cerevisiae strain W303-a
expression in Saccharomyces cerevisaiae
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expressed in Saccharomyces cerevisiae
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expression in Saccharomyces cerevisiae
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expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae
plasmid coding for rat delta6-desaturase constructed using pCMV for expression in mammalian cells, rat delta6-desaturase sequence, GenBank accession number AB02 1980 PCR amplified, expressed by transiently transforming COS-7 cells
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RT-PCR analysis of INS-1 beta-cell content of mRNA, primer pairs basedon known rat sequence
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expressed in Saccharomyces cerevisiae strain INVScl
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expressed in Saccharomyces cerevisiae strain InvSc1
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expression in Saccharomyces cerevisiae
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gene DELTA6fad_a, several variants, genetic structure, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae; gene DELTA6fad_b, several variants, genetic structure, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae; gene DELTA6fad_c, several variants, genetic structure, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisaiae
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expressed in Saccharomyces cerevisiae strain InvSc1
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expressed in Pichia pastoris GS115; phylogenetic tree, expression in Pichia pastoris GS115, molecular mechanisms underlying the elevated expression of fatty acid desaturases induced by a decrease in the environmental temperature
expression of N-terminal truncated enzyme in Escherichia coli
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expression of wild-type, A181T/A188G/Y189F/S205N/L206T/G207A, A188G/Y189F, A181T/A200F and A181T/A200F/S205N/L206T/G207A mutant enzyme in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
H129G
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole
H129R
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate
H305G
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole
H305R
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate
H89G
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity, can be rescued by 150 mM exogenous imidazole
H89R
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity using linoleic acid as substrate
H129G
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole
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H129R
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate
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H305G
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole
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H89G
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity, can be rescued by 150 mM exogenous imidazole
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G390D
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amino acid replacement in isoenzyme DELTA6I of DELTA6 desaturase-defective mutant strain YB214
T375K
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amino acid replacement in isoenzyme DELTA6I of DELTA6 desaturase-defective mutant strain HR95
W314Stop
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amino acid replacement in isoenzyme DELTA6I of DELTA6 desaturase-defective mutant strain ST66
A181T/A188G/Y189F/S205N/L206T/G207A
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compared to wild-type: reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate, exhibits DELTA9 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate
A181T/A200F
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increase in DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, strong DELTA9 desaturase activity with stearoyl-[acyl-carrier protein] as substrate
A181T/A200F/S205N/L206T/G207A
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reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate, very low DELTA9 desaturase activity, no DELTA6 desaturase activity with stearoyl-[acyl-carrier protein] as substrate
A188G/Y189F
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reduced DELTA6 desaturase activity with palmitoyl-[acyl-carrier protein] as substrate
H89R
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expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity using linoleic acid as substrate
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additional information
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genetic polymorphism of DELTA6 desaturase are naturally occuring in humans, a delivery of the gene for D6 desaturase to endothelial cells at atherosclerosis prone areas is expected to prevent/arrest the development of atherosclerosis despite the presence of hyperlipidemia, hypertension, diabetes mellitus, and at sites of shear stress of blood flow
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
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DELTA 6-fatty acid desaturase is used in the synthesis of polyunsaturated fatty acids from microorganisms to higher animals, including arachidonic acid and eicosapentaenoic acid
drug development
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the enzyme is a biological target for the discovery and development of pharmaceuticals to treat atherosclerosis
synthesis
Oenothera biennis is grown commercially for gamma-linolenic acid production by the fatty acid DELTA6-desaturase enzymatic reaction