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Information on EC 1.14.19.25 - acyl-lipid omega-3 desaturase (cytochrome b5)
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EC Tree
1.14.19.25 acyl-lipid omega-3 desaturase (cytochrome b5)IUBMB Comments
This microsomal enzyme introduces a cis double bond three carbons away from the methyl end of a fatty acid incorporated into a glycerolipid. The distance from the carboxylic acid end of the molecule does not have an effect. The plant enzyme acts on carbon 15 of linoleoyl groups incorporated into both the sn-1 and sn-2 positions of the glycerol backbone of phosphatidylcholine and other phospholipids, converting them into alpha-linolenoyl groups. The enzyme from the fungus Mortierella alpina acts on gamma-linolenoyl and arachidonoyl groups, converting them into stearidonoyl and icosapentaenoyl groups, respectively . cf. EC 1.14.19.35, sn-2 acyl-lipid omega-3 desaturase (ferredoxin).
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Word Map
- 1.14.19.25
-
linolenic
-
polyunsaturated
-
desaturases
-
pufas
-
alpha-linolenic
-
desaturation
- flax
- usitatissimum
-
linum
-
trienoic
-
omega-6
- stearoyl-acp
- medicine
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
omega-3 fatty acid desaturase, fad3b, omega3-desaturase, fatty acid desaturase 3, fad3c, sdd17, lefad3, msdd17, omega-3 fad, omega-3 fa desaturase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DELTA15 desaturase
endoplasmic reticulum-type omega-3 fatty acid desaturase
ER-type desaturase
FAD3
FAD3A
FAD3B
fatty acid desaturase 3
maw3
omega-3 desaturase
omega-3 FA desaturase
omega-3 FAD
omega-3 fatty acid desaturase
omega3-desaturase
FAD3
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = an alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
-
, , ,
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SYSTEMATIC NAME
IUBMB Comments
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid],ferrocytochrome b5:oxygen oxidoreductase (15,16 cis-dehydrogenating)
This microsomal enzyme introduces a cis double bond three carbons away from the methyl end of a fatty acid incorporated into a glycerolipid. The distance from the carboxylic acid end of the molecule does not have an effect. The plant enzyme acts on carbon 15 of linoleoyl groups incorporated into both the sn-1 and sn-2 positions of the glycerol backbone of phosphatidylcholine and other phospholipids, converting them into alpha-linolenoyl groups. The enzyme from the fungus Mortierella alpina acts on gamma-linolenoyl and arachidonoyl groups, converting them into stearidonoyl and icosapentaenoyl groups, respectively [3]. cf. EC 1.14.19.35, sn-2 acyl-lipid omega-3 desaturase (ferredoxin).
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
arachidonic acid + 2 ferrocytochrome b5 + O2 + 2 H+
eicosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
eicosapentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
omega-6 linoleic acid + 2 ferrocytochrome b5 + O2 + 2 H+
linolenic acid + 2 ferricytochrome b5 + 2 H2O
-
best substrate
-
-
?
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
additional information
?
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
additional information
?
-
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
additional information
?
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
additional information
?
-
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Mycoses
Rapid, transient, and highly localized induction of plastidial omega-3 fatty acid desaturase mRNA at fungal infection sites in Petroselinum crispum.
Neoplasms
An ?-3 fatty acid desaturase-expressing gene attenuates prostate cancer proliferation by cell cycle regulation.
Prostatic Neoplasms
An ?-3 fatty acid desaturase-expressing gene attenuates prostate cancer proliferation by cell cycle regulation.
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
high activity and/or abundant expression in developing seeds
brenda
additional information
tissue-specific expression, overview
brenda
additional information
tissue expression analysis of gene fad3, overview
brenda
additional information
-
tissue expression analysis of gene fad3, overview
brenda
additional information
expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview
brenda
additional information
expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview
brenda
additional information
expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview. No expression in stem, root, and flower
brenda
additional information
expression pattern analysis, fatty acid profiles of perilla leaves and mature seeds, overview. No expression in stem, root, and flower
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
brenda
additional information
additional information
subcellular localization analysis, overview
-
brenda
additional information
subcellular localization analysis, overview
-
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
accumulation of linoleoyl (18:2) groups at the expense of linolenoyl groups (18:3) is the only substantial difference in membrane lipid biosynthesis in leaves and roots of fad3-2 plants relative to wild type
metabolism
physiological function
additional information
metabolism
regulation of the enzyme through synergistic and antagonistic hormonal interactions, overview
metabolism
-
regulation of the enzyme through synergistic and antagonistic hormonal interactions, overview
-
physiological function
OMEGA-3 fatty acid desaturase converts exogenous arachidonic acid, an OMEGA-6 PUFA, into eicosapentaenoic acid (EPA). Gene sdd17 from EPA-rich fungus is expressed at high levels and increases OMEGA-3 fatty acid concentrations in mammalian cells
physiological function
the omega-3 FAD gene expression product and its enzymatic product C18:3n3 are crucial to the microalgae cell system
physiological function
-
tomato endoplasmic reticulum-type omega-3 fatty acid desaturase functions in early seedling tolerance to salinity stress
physiological function
Chlorella vulgaris UMT-M1
-
the omega-3 FAD gene expression product and its enzymatic product C18:3n3 are crucial to the microalgae cell system
-
additional information
fatty acid profile of wild-type and fad3 mutant roots in response to several plant hormones, overview
additional information
-
fatty acid profile of wild-type and fad3 mutant roots in response to several plant hormones, overview
additional information
the enzyme contains the conserved His box of plant omega-3 FA desaturase. One amino acid residue, Asn or Ser, is different at 61 position of PfrFAD3-1 and PfrFAD3-2, respectively. Both PfrFAD3-1 and -2 have the function of omega-3 FA desaturases
additional information
the enzyme contains the conserved His box of plant omega-3 FA desaturase. One amino acid residue, Asn or Ser, is different at 61 position of PfrFAD3-1 and PfrFAD3-2, respectively. Both PfrFAD3-1 and -2 have the function of omega-3 FA desaturases
additional information
-
fatty acid profile of wild-type and fad3 mutant roots in response to several plant hormones, overview
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). FAD3E_ARATH
386
3
44077
Swiss-Prot
other Location (Reliability: 2)
FAD3E_BRANA
383
5
43936
Swiss-Prot
other Location (Reliability: 2)
FAD3E_MORAP
403
6
46465
Swiss-Prot
other Location (Reliability: 2)
A0A5B6Z354_DAVIN
334
3
38593
TrEMBL
other Location (Reliability: 5)
A0A5B6Z0F9_DAVIN
236
1
26807
TrEMBL
other Location (Reliability: 5)
A0A812CI62_SEPPH
380
6
44920
TrEMBL
Secretory Pathway (Reliability: 5)
A0A7R8H7D8_LEPSM
237
3
26855
TrEMBL
other Location (Reliability: 2)
A0A5B6Z1X1_DAVIN
432
3
49702
TrEMBL
other Location (Reliability: 5)
A0A5B6Z0F2_DAVIN
427
3
49037
TrEMBL
other Location (Reliability: 5)
A0A2Z4N5R7_PHYFE
378
4
43490
TrEMBL
other Location (Reliability: 1)
A0A5B6Z016_DAVIN
427
3
48885
TrEMBL
other Location (Reliability: 5)
W0S9D5_FISSO
422
6
48251
TrEMBL
Mitochondrion (Reliability: 5)
W0SDG6_FISSO
421
6
48197
TrEMBL
Secretory Pathway (Reliability: 5)
A0A2Z4N5R8_PHYFE
387
3
44183
TrEMBL
other Location (Reliability: 2)
A0A650C598_9BORA
425
3
48989
TrEMBL
other Location (Reliability: 2)
A0A7R8CBH7_LEPSM
462
5
52915
TrEMBL
other Location (Reliability: 3)
Q84X83_SOYBN
380
0
44136
TrEMBL
other Location (Reliability: 4)
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
food industry
-
combinations of mutations in the three FAD3 enzyme genes are capable of producing soybean seeds containing only 1% linolenic acid in the seed oil
additional information
additional information
seed fatty acid profiles of tobacco showing alteration in transgenic lines compared to the untransformed control, analysis of transgenic rice seed fatty acid profiles, overview
additional information
-
a mutant protein lacking 110 amino acids at its C terminus exhibits a drastic decline in its DELTA-15 desaturase activity compared to the wild type protein
additional information
omega-3 FAD gene disruption and stable recombinant expression of the gene in Chlorella vulgaris strain UMT-M1 via Agrobacterium-mediated transformation. Effects of disrupted omega-3 desaturase gene construct on fatty acid composition and expression of four fatty acid biosynthetic genes in transgenic Chlorella vulgaris, overview. The wild-type and transgenic mutant lines cultured under nitrate-deficient media display no significant differences. Re-emergence of the omega-3 FAD mRNA transcript subsequent to six months of cell culture maintenance
additional information
Chlorella vulgaris UMT-M1
-
omega-3 FAD gene disruption and stable recombinant expression of the gene in Chlorella vulgaris strain UMT-M1 via Agrobacterium-mediated transformation. Effects of disrupted omega-3 desaturase gene construct on fatty acid composition and expression of four fatty acid biosynthetic genes in transgenic Chlorella vulgaris, overview. The wild-type and transgenic mutant lines cultured under nitrate-deficient media display no significant differences. Re-emergence of the omega-3 FAD mRNA transcript subsequent to six months of cell culture maintenance
-
additional information
-
generation of transgenic FAD3-antisense and FAD3-sense plants, penotypes, overview
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene fad3, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant plamid pCAM::Nap-BjFad3-NOS is introduced into Agrobacterium tumefaciens strain LBA4404/VirGN54D by freeze-thaw method and then used for transformation of Escherichia coli strain DH10B and of Nicotiana tabacum explant, recombinant ectopic expression analysis in leaves of transgenic plants, recombinant expression of the enzyme in transgenic Oryza sativa plants, semi-quantitative RT-PCR expression analysis
gene fad3, overexpression of sense and antisense FAD3 sequences under control of the CaMV 35S promoter, quantitative reverse transcription-PCR enzyme expression analysis, LeFAD3 overexpression enhances the tolerance of early seedlings to salinity stress. Reduced oxidative damage in LeFAD3 overexpression tomato plants under salt stress, with altered ratio of 18:3/18:2 compared to wild-type. Change in leaf cell ultrastructure under salt stress in wild-type and mutant leaves
-
gene fad3, recombinant expression of a fad3-LUC fusion construct in Arabidopsis thaliana plants through trabsfection with Agrobacterium tumefaciens strain C58 pGV3850
gene fad3-1, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene fad3-2, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene omega-3 FAD, genetic structure with endogenous gene and native promoter, recombinant expression of the disrupted omega-3 FAD gene in Chlorella vulgaris via Agrobacterium-mediated transformation, real-time PCR and RT-PCR enzyme expression analysis
gene sdd17, recombinant exression of codon-otimized gene in human VCaP prostate cancer cells by lentiviral tranfection using the the PLJM1 lentivirus vector, which allows the endogenous production of OMEGA-3 polyunsaturated fatty acids (PUFAs), quantitative RT-PCR expression analysis
isolation of a gene and functionally complementation of a mutant of Arabidopsis deficient in omega-3 unsaturated fatty acids
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
auxin and cytokinin induce the enzyme expression ectopically in roots when applied during vegetative growth but not during repoductive growth or germination, the induction is repressed by abscisic acid
salt stress suppresses the growth of wild-type and FAD3-antisense plants to a higher extent than that in FAD3-sense plants
-
auxin and cytokinin induce the enzyme expression ectopically in roots when applied during vegetative growth but not during repoductive growth or germination, the induction is repressed by abscisic acid
auxin and cytokinin induce the enzyme expression ectopically in roots when applied during vegetative growth but not during repoductive growth or germination, the induction is repressed by abscisic acid
-
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
consuming high levels of long-chain OMEGA-3 polyunsaturated fatty acids (PUFAs) is been shown to reduce prostate cancer risk and increase chemotherapy sensitivity in humans
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sakuradani, E.; Abe, T.; Iguchi, K.; Shimizu, S.
A novel fungal omega3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4
Appl. Microbiol. Biotechnol.
66
648-654
2005
Mortierella alpina (Q59J82), Mortierella alpina, Mortierella alpina 1S-4 (Q59J82), Mortierella alpina 1S-4
brenda
Browse, J.; McConn, M.; James, D. Jr.; Miquel, M.
Mutants of Arabidopsis deficient in the synthesis of alpha-linolenate. Biochemical and genetic characterization of the endoplasmic reticulum linoleoyl desaturase
J. Biol. Chem.
268
16345-16351
1993
Arabidopsis thaliana
brenda
Arondel, V.; Lemieux, B.; Hwang, I.; Gibson, S.; Goodman, H.M.; Somerville, C.R.
Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis
Science
258
1353-1355
1992
Brassica napus (P48624)
brenda
Singer, S.; Weselake, R.; Rahman, H.
Development and characterization of low alpha-linolenic acid Brassica oleracea lines bearing a novel mutation in a class a FATTY ACID DESATURASE 3 gene
BMC Genet.
15
94
2014
Brassica oleracea
brenda
Bilyeu, K.; Gillman, J.; LeRoy, A.
Novel FAD3 mutant allele combinations produce soybeans containing 1% linolenic acid in the seed oil
Crop Sci.
51
259-264
2011
Glycine max
brenda
Hamada, T.; Kodama, H.; Nishimura, M.; Iba, K.
Cloning of a cDNA encoding tobacco omega-3 fatty acid desaturase
Gene
147
293-294
1994
Nicotiana tabacum (P48626), Nicotiana tabacum
brenda
Savile, C.; Reed, D.; Meesapyodsuk, D.; Covello, P.; Buist, P.
Cryptoregiochemistry of a Brassica napus fatty acid desaturase (FAD3): A kinetic isotope effect study
J. Chem. Soc. Perkin Trans. I
2001
1116-1121
2001
Brassica napus
-
brenda
Khadake, R.; Khonde, V.; Mhaske, V.; Ranjekar, P.; Harsulkar, A.
Functional and bioinformatic characterisation of sequence variants of Fad3 gene from flax
J. Sci. Food Agric.
91
2689-2696
2011
Linum usitatissimum
brenda
Radovanovic, N.; Thambugala, D.; Duguid, S.; Loewen, E.; Cloutier, S.
Functional characterization of flax fatty acid desaturase FAD2 and FAD3 isoforms expressed in yeast reveals a broad diversity in activity
Mol. Biotechnol.
56
609-620
2014
Linum usitatissimum
brenda
Matsuda, O.; Watanabe, C.; Iba, K.
Hormonal regulation of tissue-specific ectopic expression of an Arabidopsis endoplasmic reticulum-type omega-3 fatty acid desaturase (FAD3) gene
Planta
213
833-840
2001
Arabidopsis thaliana (P48623), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (P48623)
brenda
Zhang, H.; Bi, Y.; Liu, Z.; Shan, L.
Heterologous expression of two Glycine max omega-3 fatty acid desaturases in Saccharomyces cerevisiae
Russ. J. Plant Physiol.
56
569-574
2009
Glycine max (Q84X83), Glycine max (Q84X84), Glycine max (Q9FXK9)
-
brenda
Lau, C.; Loh, S.; Aziz, A.; Cha, T.
Effects of disrupted omega-3 desaturase gene construct on fatty acid composition and expression of four fatty acid biosynthetic genes in transgenic Chlorella vulgaris
Algal Res.
26
143-152
2017
Chlorella vulgaris (A0A1L4BMI8), Chlorella vulgaris UMT-M1 (A0A1L4BMI8)
-
brenda
Pan, J.; Zhou, S.; Xiang, R.; Zhao, Z.; Liu, S.; Ding, N.; Gong, S.; Lin, Y.; Li, X.; Bai, X.; Li, F.; Zhao, A.Z.
An OMEGA-3 fatty acid desaturase-expressing gene attenuates prostate cancer proliferation by cell cycle regulation
Oncol. Lett.
13
3717-3721
2017
Saprolegnia diclina (Q6UB73)
brenda
Wang, H.; Yu, C.; Tang, X.; Zhu, Z.; Ma, N.; Meng, Q.
A tomato endoplasmic reticulum (ER)-type omega-3 fatty acid desaturase (LeFAD3) functions in early seedling tolerance to salinity stress
Plant Cell Rep.
33
131-142
2014
Solanum lycopersicum
brenda
Lee, K.R.; Lee, Y.; Kim, E.H.; Lee, S.B.; Roh, K.H.; Kim, J.B.; Kang, H.C.; Kim, H.U.
Functional identification of oleate 12-desaturase and omega-3 fatty acid desaturase genes from Perilla frutescens var. frutescens
Plant Cell Rep.
35
2523-2537
2016
Perilla frutescens var. frutescens (Q8VWX1), Perilla frutescens var. frutescens (Q9ZPP7)
brenda
Bhattacharya, S.; Chattopadhyaya, B.; Koduru, L.; Das, N.; Maiti, M.
Heterologous expression of Brassica juncea microsomal omega-3 desaturase gene (BjFad3) improves the nutritionally desirable omega-6 omega-3 fatty acid ratio in rice bran oil
Plant Cell Tissue Organ Cult.
119
117-129
2014
Brassica juncea (E0A920)
-
brenda
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EXTERNAL LINKS (specific for EC-Number 1.14.19.25)
Transporter Classification Database (TCDB): 9.A.76.1.1
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Release 2023.1 (January 2023)
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