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10-(heptyloxy)-decanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hydroxynonanoyl-[acyl-carrier protein] + n-octanal + acceptor + H2O
-
-
?
7-(decyloxy)-heptanoyl-[acyl-carrier protein] + reduced acceptor + O2
7-dec-1-enyloxyheptanoyl-[acyl-carrier protein] + acceptor + H2O
-
-
?
8-(nonyloxy)-octanoyl-[acyl-carrier protein] + reduced acceptor + O2
8-hydroxyoctanoyl-[acyl-carrier protein] + 1-nonanal + acceptor + H2O
-
-
?
9-(octyloxy)-nonanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hydroxynonanoyl-[acyl-carrier protein] + 1-octanal + acceptor + H2O
-
-
?
heptadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2
9-heptadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
very low activity, vegetative ferredoxin from Anabaena
-
?
hexadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2 + H+
9-hexadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
-
-
r
nonadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2
9-nonadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
low activity, vegetative ferredoxin from Anabaena
-
?
octadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2 + H+
9-octadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
-
-
r
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
pentadecanoyl-[acyl-carrier protein] + reduced ferredoxin + O2
9-pentadecenoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
very low activity, vegetative ferredoxin from Anabaena
-
?
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
stearoyl-[acyl-carrier protein] + ferredoxin + O2
oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
9-octadecenoyl-[acyl-carrier protein] + acceptor + H2O
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
stearoyl-[acyl-carrier protein] + reduced ferredoxin + O2 + H+
oleoyl-[acyl-carrier protein] + oxidized ferredoxin + H2O
-
-
-
r
stearoyl-[acyl-carrier protein] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
oleoyl-[acyl-carrier protein] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
additional information
?
-
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
preferred substrate for S-ACP-DES3, S-ACP-DES3 preferentially desaturates palmitoyl-[acyl-carrier protein] substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
S-ACP-DES1 is poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES1 preferentially desaturates the substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
S-ACP-DES4 is poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES4 preferentially desaturates the substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
S-ACP-DES5 poorly active on palmitoyl-[acyl-carrier protein], S-ACP-DES5 preferentially desaturates palmitoyl-[acyl-carrier protein] substrate at C9 position
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
8% of activity with stearoyl-[acyl-carrier-protein], 7fold higher activity than the enzyme from castor
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
Bignonia unguis-cati
4fold lower activity with stearoyl-[acyl-carrier protein] and myristoyl-[acyl-carrier protein]
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
1% of activity with stearoyl-[acyl-carrier-protein]
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
stearoyl-substrate is about 100fold preferred over palmitoyl-substrate
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
9-hexadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
-
-
?
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
-
-
?
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
5% of activity with stearoyl-[acyl-carrier-protein]
-
?
stearoyl-CoA + electron donor + O2
9-octadecenoyl-CoA + acceptor + H2O
-
purified reconstituted enzyme shows same activity as with stearoyl-[acyl-carrier-protein], no activity in crude extracts
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
involved in oleic acid biosynthesis
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
involved in oleic acid biosynthesis
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?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
enzyme catalyzes the principal conversion of saturated fatty acids to unsaturated fatty acids in synthesis of vegetable oils
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
preferred substrate for S-ACP-DES1, S-ACP-DES1 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
preferred substrate for S-ACP-DES5, S-ACP-DES5 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
S-ACP-DES3 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
very low activity of S-ACP-DES4, S-ACP-DES4 preferentially desaturates the substrate at C9 position
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
9-octadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
9-octadecenoyl-[acyl-carrier protein] + acceptor + H2O
-
stearoyl-substrate is about 100fold preferred over palmitoyl-substrate
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
no activity with NADH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
a system composed of ferredoxin, grana lamellae, ascorbic acid, dichlorophenolindophenol and light is the most effective reductant
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
electron donor NADPH-ferredoxin(II)
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
enzyme is specific for stearoyl-CoA
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
enzyme is specific for stearoyl-CoA
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
no activity with NADH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
purified enzyme is not active with NADH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
enzyme acts on derivatives of a number of long chain fatty acids
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
electron donor NADPH
-
?
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
oleoyl-[acyl-carrier protein] + acceptor + H2O
-
electron donor NADPH
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
-
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
-
-
-
?
tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
-
3% of activity with stearoyl-[acyl-carrier-protein], 30fold higher activity than castor enzyme
-
?
tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
-
3fold more active as with palmitoyl-[acyl-carrier protein], very low activity with dodecanoyl-[acyl-carrier protein] and stearoyl-[acyl-carrier protein]
-
?
tetradecanoyl-[acyl-carrier protein] + reduced acceptor + O2
9-tetradecenoyl-[acyl-carrier protein] + acceptor + H2O
-
-
-
?
additional information
?
-
-
in vitro analysis of the enzyme does not show any product
-
-
?
additional information
?
-
-
lack of soluble OeSAD2 protein expression precludes its in vitro analysis
-
-
?
additional information
?
-
-
OsSAD2 is active both on 18:0-[acyl-carrier protein] DELTA9 and 16:0-[acyl-carrier protein] DELTA4 substrates producing 18:1 DELTA9 and 16:1 DELTA4 products
-
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?
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0.0000016
activity in extracts from Escherichia coli M15 transformed with pQESAD, uninduced, with reduced spinach ferredoxin
0.000002
activity in extracts from Escherichia coli M15 transformed with pQESAD, uninduced, without reduced spinach ferredoxin
0.000003
His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, without IPTG-induction
0.0000075
activity in extracts from Escherichia coli M15 transformed with pQESAD, induced, with reduced spinach ferredoxin
0.000079
activity in extracts from Escherichia coli M15 transformed with pQESAD, induced, without reduced spinach ferredoxin
0.000082
His-tag fusion protein overexpressed in Escherichia coli M15 without ferredoxin, IPTG-induced
0.00157
His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induction
0.00795
His-tag fusion protein overexpressed in Escherichia coli M15 with exogenous spinach ferredoxin, without IPTG-induced
additional information
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES1 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES3 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES4 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
S-ACP-DES5 shows no activity on the substrates myristoyl-[acyl-carrier protein], palmitoleoyl-[acyl-carrier protein] or oleoyl-[acyl-carrier protein]
additional information
assay procedure
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-
brenda
young
brenda
low enzyme content
brenda
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RT-PCR, gene-specific primers for the isoform
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high enzyme content
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RT-PCR, gene-specific primers for the isoform
brenda
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highly expressed in developing fruits
brenda
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brenda
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brenda
Northern blot, RT-PCR, gene-specific primers for the isoform
brenda
Northern blot, RT-PCR, gene-specific primers for the seven isoforms
brenda
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49000 Da isoform
brenda
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wild-type and mutant leaf phenotypes, overview
brenda
young and mature
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low enzyme content
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developing
brenda
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flax ovary, RT-PCR
brenda
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brenda
RT-PCR, gene-specific primers for the isoform
brenda
young
brenda
-
expressed at the lateral root tip, but not detected in the elongation zone of the crown roots
brenda
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2 isoforms
brenda
Bignonia unguis-cati
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found in developing seeds, 15-50 days after flowering, not in germinated seeds
brenda
young
brenda
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-
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zygotic, TcSAD relative expression and fatty acid composition in maturing cacao seeds, overview
brenda
zygotic, TcSAD relative expression and fatty acid composition in maturing cacao seeds, overview, highest expression level of TcSAD7
brenda
zygotic, TcSAD relative expression and fatty acid composition in maturing cacao seeds, overview. High expression level of TcSAD1
brenda
-
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brenda
expression levels of ZmSAD1 in the mature maize embryos, ten maize embryos are excised from the mature seeds, overview
brenda
RT-PCR, gene-specific primers for the isoform
brenda
young
brenda
additional information
the yield of CocoFAD is the highest in the endosperm of 8-month-old coconut and leaf, the yield is reduced to 50% of the highest level in the endosperm of 15-month-old coconut
brenda
additional information
-
bolls at different developmental stages, RT-PCR
brenda
additional information
fatty acid content and SAD isozyme expression levels during fruit mesocarp development, overview
brenda
additional information
fatty acid content and SAD isozyme expression levels during fruit mesocarp development, overview
brenda
additional information
fatty acid content and SAD isozyme expression levels during fruit mesocarp development, overview
brenda
additional information
quantitative real-time PCR reveals that the gene shows marked distinct expression during different stages of seed developments. SAD gene displays tissue-specific expression patterns, the expression level in seeds and in leaf is significantly higher than in other tissues
brenda
additional information
-
quantitative real-time PCR reveals that the gene shows marked distinct expression during different stages of seed developments. SAD gene displays tissue-specific expression patterns, the expression level in seeds and in leaf is significantly higher than in other tissues
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
-
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, expression of TcSAD5 is primarily detected in roots at low level
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
-
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, genes TcSAD3 and TcSAD4 are predominantly expressed in unopened and open flowers, where the expression levels of TcSAD3 and TcSAD4 are significantly higher than any other SAD isoforms, the transcripts of both are barely detectable in all the other tissues, indicating that they are flower-specific genes
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
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tissue-specific expression pattern of SAD isoforms in Theobroma cacao, TcSAD7 constitutivel expresses in all examined tissues with higher expression in roots and zygotic seeds at all developmental stages
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
-
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD1 and TcSAD2 are detected in all the examined tissues, in which the expression levels of TcSAD1 are higher than those of TcSAD2
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
-
tissue-specific expression pattern of SAD isoforms in Theobroma cacao, transcripts of TcSAD6 and TcSAD8 are barely detected in any of the tissues analyzed, suggesting that they might be pseudogenes or expressed in a tissue, stage of development, or induction condition not tested
brenda
additional information
XsSAD Xanthoceras sorbifolia has a much higher expression in embryos than in leaves and petals. XsSAD expression also correlated well with the oleic acid, unsaturated fatty acid, and total fatty acid levels in developing embryos
brenda
additional information
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XsSAD Xanthoceras sorbifolia has a much higher expression in embryos than in leaves and petals. XsSAD expression also correlated well with the oleic acid, unsaturated fatty acid, and total fatty acid levels in developing embryos
brenda
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malfunction
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Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. None of the transgenic plant lines complement the dwarf phenotype of homozygous ssi2 mutants. The presence of the OsSAD2 transgene is significantly associated with changes in unsaturated C18 and C16 FA levels in Arabidopsis leaf lipids, suggesting that OsSAD2 has enzymatic activity in Arabidopsis
malfunction
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Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OeSAD2 does not complement the dwarf phenotype of homozygous ssi2 mutants
malfunction
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Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OsSAD1 does not complement the dwarf phenotype of homozygous ssi2 mutants
malfunction
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a stearoyl-acyl carrier protein fatty acid desaturase mutant has decreased lateral root growth due to a defect in the cell elongation
malfunction
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seed homozygous for the SACPD-C deletion averages 10.4% stearic acid and 75.9% oleic acid
malfunction
downregulation of ZmSAD1 increases the stearic acid concentration in maize leaf
malfunction
fatty acid composition of wild-type and mutant plants, overview
malfunction
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one nonsense and four missense Gmsacpd-c mutants are identified to have high levels of seed, nodule, and leaf stearic acid content. Homology modeling and in silico analysis of the GmSACPD-C enzyme reveals that most of these mutations are localized near or at conserved residues essential for di-iron ion coordination. Soybeans carrying Gmsacpd-c mutations at conserved residues cause the highest stearic acid content, and these mutations have deleterious effects on nodule development and function. Mutant plants with mutations at nonconserved residues show an increase in stearic acid content yet retain healthy nodules. Nodule leg hemoglobin transcripts are significantly more abundant in soybeans with deleterious mutations at conserved residues of GmSACPD-C. Gmsacpd-c mutations cause an increase in leaf stearic acid content and an alteration of leaf structure and morphology in addition to differences in nitrogen-fixing nodule structure. Wild-type and mutant leaf phenotypes, overview
malfunction
the isozyme A-C triple knockdown plants display severe growth phenotypes, including spontaneous cell death and dwarfing. While no vegetative morphologic abnormality is observed in individual NbSACPD-A, -B, or -C knockdown plants, strikingly, NbSACPD-C knockdown plants produce small fruits with aborted ovules. Reciprocal crosses with wild-type and NbSACPD-C knockdown plants reveal that knocking down NbSACPD-C expression causes female, but not male, sterility. Arrested ovule development and significantly altered lipid composition in ovaries are observed in NbSACPD-C knockdown plants, consistent with the predominant NbSACPD-C expression in ovules. The ovule development defect is fully complemented by coexpressing an amiRNA-resistant NbSACPD-C variant in the NbSACPD-C knockdown background, further supporting a specific requirement for NbSACPD-C in female fertility. Phenotypes, overview
malfunction
the isozyme A-C triple knockdown plants display severe growth phenotypes, including spontaneous cell death and dwarfing. While no vegetative morphologic abnormality is observed in individual NbSACPD-A, -B, or -C knockdown plants, strikingly, NbSACPD-C knockdown plants show a highly altered phenotype, overview. Phenotypes, overview
malfunction
transgenic maize that expresses high levels of ZmSAD1 in its mature seeds shows reduced stearic acid content (1.57%) and a lower saturated to unsaturated fatty acid ratio (20.40%) relative to those (1.64% and 20.61%, respectively) of the control. Conversely, downregulation of ZmSAD1 in maize results in increased levels of stearic acid (1.78%), long-chain saturated acids (0.85%) and the ratio of saturated to unsaturated fatty acids (21.54%) relative to those (1.64%, 0.74%, and 20.61%, respectively) of the control, whereas the oleic acid (32.01%) level is significantly decreased relative to that (32.68%) of the control
metabolism
the enzyme catalyzes the conversion of stearic acid (18:0) to oleic acid (18:1) in fatty acid biosynthesis. Enzyme overexpression enhances the synthesis of oleic acid 2.4fold
metabolism
under normal growth conditions, the stearoyl-ACP molecule is generally rapidly desaturated by SAD to form oleoyl-ACP inside the chloroplasts resulting in low presence of stearic acid in glycerolipids. The further desaturation or elongation of oleoyl-ACP into polyunsaturated fatty acids (PUFAs) can occur in either the chloroplast using the prokaryotic pathway, or the acyl-ACP molecules are exported outside the chloroplast as acyl-CoA molecules and enter the eukaryotic pathway
metabolism
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under normal growth conditions, the stearoyl-ACP molecule is generally rapidly desaturated by SAD to form oleoyl-ACP inside the chloroplasts resulting in low presence of stearic acid in glycerolipids. The further desaturation or elongation of oleoyl-ACP into polyunsaturated fatty acids (PUFAs) can occur in either the chloroplast using the prokaryotic pathway, or the acyl-ACP molecules are exported outside the chloroplast as acyl-CoA molecules and enter the eukaryotic pathway
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physiological function
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SAD2 is a florally expressed barrier gene of large phenotypic effect and, possibly, a genic target of pollinator-mediated selection
physiological function
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the enzyme is involved in cell elongation in lateral roots via regulation of fatty acid content in rice
physiological function
activity of TcSAD1 is possibly involved in the synthesis and accumulation of oleate in cacao embryos
physiological function
all three isozymes of SAD participate in fatty acid desaturation in Nicotiana benthamiana, roles of SACPD isozymes in biotic and abiotic stresses
physiological function
lipid content and gene expression analyses indicate that isozyme OeSAD2 seems to be the main gene contributing to the oleic acid content of the olive fruit and, therefore, of the virgin olive oil
physiological function
lipid content and gene expression analyses of SAD isozymes indicate that isozyme OeSAD2 seems to be the main gene contributing to the oleic acid content of the olive fruit and, therefore, of the virgin olive oil. The olive microsomal oleate desaturase gene OeFAD2-2, but not OeSAD2, is responsible for the linoleic acid content in the virgin olive oil
physiological function
lipid content and gene expression analyses of SAD isozymes of SAD isozymes indicate that isozyme OeSAD2 seems to be the main gene contributing to the oleic acid content of the olive fruit and, therefore, of the virgin olive oil
physiological function
stearoyl-ACP desaturase (SAD) is a key rate-limiting enzyme for the conversion of stearic (C18:0) to oleic (C18:1) acid, analysis of C18:0/C18:1 ratio in 11 different genotypes of Zea mays SAD plants, overview. Stearic and oleic acid concentration can be regulated by ZmSAD1 expression
physiological function
stearoyl-ACP desaturase is a plastid-localized soluble desaturase that catalyzes the conversion of stearic acid (18:0) to oleic acid, which plays a key role in determining the ratio of saturated to unsaturated fatty acids
physiological function
the enzyme converts stearic acid into oleic acid
physiological function
the enzyme may be involved in the regulation of plant seed growth and development
physiological function
the stearoyl-acyl carrier protein (ACP) desaturase (SAD), a plastidial DELTA9 desaturase from the endosperm of coconut, plays a key role in the properties of the majority of cellular glycerolipids. The DELTA9 fatty acid desaturase is a critical enzyme in the synthesis of unsaturated fatty acids, which has the capability to convert palmitic acid (16:0) or stearic acid (18:0) into palmitoleic acid (16:1) or oleic acid (18:1), respectively
physiological function
the stearoyl-acyl carrier protein desaturase, NbSACPD-C, is critical for ovule development, all three isozymes of SAD participate in fatty acid desaturation in Nicotiana benthamiana, roles of SACPD isozymes in biotic and abiotic stresses
physiological function
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the enzyme converts stearic acid into oleic acid
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additional information
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homology modeling of GmSACPD-C from cv. Forrest with important catalytic residues and the five identified sacpd-c missense mutations mapped, overview
additional information
oleic acid is the main fatty acid in mesocarp and seed tissues
additional information
oleic acid is the main fatty acid in mesocarp and seed tissues
additional information
oleic acid is the main fatty acid in mesocarp and seed tissues
additional information
seed-specific overexpression of the exogenous ZmSAD1 gene in Arabidopsis thaliana significantly reduces the content of stearic acid and the ratio of saturated to unsaturated fatty acids
additional information
three dimensional structure modeling, the enzyme PtSAD contains conserved domains and is a stable hydrophilic protein
additional information
three dimensional structure molecular homology modeling and structure comparison, overview
additional information
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three dimensional structure molecular homology modeling and structure comparison, overview
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C235T
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random mutagenesis
C247T
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random mutagenesis
C305T
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random mutagenesis
D77N
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random mutagenesis, the alteration of charge in the missense mutants SACPD-CD77N is due to the iron ion pocket localization, the mutation is predicted to affect iron ion-binding kinetics and stability
E114K
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random mutagenesis, the mutation directly alters the negatively charged bridging ligand Glu114 into a positively charged Lys
G1777A
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random mutagenesis
G1964T
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random mutagenesis
G229A
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random mutagenesis
G340A
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random mutagenesis
L79F
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random mutagenesis, the alteration of charge in the missense mutants SACPD-CD77N is due to the iron ion pocket localization, presence of steric hindrance by L79F, the mutation is predicted to affect iron ion-binding kinetics and stability
P102L
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random mutagenesis, the missense mutant SACPD-CP102L is not localized at the iron ion-binding pocket but is positioned at the first residue of the alpha4 chain,which holds the ligands Glu114 and His117 in place. Considering Pro's cyclic conformation, in which the secondary amine binds to the alha-carbon of the protein backbone, a disruption of this conformational rigidity may impact the ability of the alpha4 chain to be in its proper location, disrupting the enzymatic activity of GmSACPD-C
L118F/P179I
15fold higher activity with palmitoyl-[acyl-carrier protein] than wild-type enzyme, low DELTA10 desaturase activity
L118W
100% activity with palmitoyl-[acyl-carrier protein], 89% activity with stearoyl-[acyl-carrier protein] and 8.2% activity with myristoyl-[acyl-carrier protein], wild-type is only active with stearoyl-[acyl-carrier protein]
T117R/G188L
82fold higher specificity for palmitoyl-[acyl-carrier protein] with respect to wild-type
additional information
n CrFAB2-overexpressing lines, oleic acid (18:1) content is increased by approximately 2.4fold compared to the wild-type control plants. Gene FAB2 overexpression result in the induction of FAD2 expression. Consistent with this result, the induction of linoleic acid (18:2) is also detected in CrFAB2-overexpressing lines, and total fatty acid content in these lines is induced by approximately 28% trough CrFAB2 overexpression compared to the wild-type control. Phenotypes, overview
additional information
the enzyme is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii mutant starchless BAFJ5 mutant strain via two different constructs, which target different positions on the mRNA of stearoyl-ACP desaturase. mRNA levels for SAD are reduced after the silencing construct is induced. One strain shows reduction in SAD mRNA resulting in a doubling of the stearic acid content in triacylglycerol molecules, which shows that stearic acid production in microalgae is possible. The mRNA expression is transiently reduced upon the induction of the amiRNA construct by heat shock and nitrogen depleted growth conditions, which doubles the stearic acid content in Chlamydomonas reinhardtii
additional information
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the enzyme is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii mutant starchless BAFJ5 mutant strain via two different constructs, which target different positions on the mRNA of stearoyl-ACP desaturase. mRNA levels for SAD are reduced after the silencing construct is induced. One strain shows reduction in SAD mRNA resulting in a doubling of the stearic acid content in triacylglycerol molecules, which shows that stearic acid production in microalgae is possible. The mRNA expression is transiently reduced upon the induction of the amiRNA construct by heat shock and nitrogen depleted growth conditions, which doubles the stearic acid content in Chlamydomonas reinhardtii
additional information
-
the enzyme is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii mutant starchless BAFJ5 mutant strain via two different constructs, which target different positions on the mRNA of stearoyl-ACP desaturase. mRNA levels for SAD are reduced after the silencing construct is induced. One strain shows reduction in SAD mRNA resulting in a doubling of the stearic acid content in triacylglycerol molecules, which shows that stearic acid production in microalgae is possible. The mRNA expression is transiently reduced upon the induction of the amiRNA construct by heat shock and nitrogen depleted growth conditions, which doubles the stearic acid content in Chlamydomonas reinhardtii
-
additional information
-
one nonsense and four missense Gmsacpd-c mutants are identified to have high levels of seed, nodule, and leaf stearic acid content. Homology modeling and in silico analysis of the GmSACPD-C enzyme reveals that most of these mutations are localized near or at conserved residues essential for di-iron ion coordination. Soybeans carrying Gmsacpd-c mutations at conserved residues show the highest stearic acid content, and these mutations have deleterious effects on nodule development and function. Nodule leghemoglobin transcripts are significantly more abundant in soybeans with deleterious mutations at conserved residues of GmSACPD-C. Gmsacpd-c mutations cause an increase in leaf stearic acid content and an alteration of leaf structure and morphology in addition to differences in nitrogen-fixing nodule structure. Wild-type and mutant leaf phenotypes, overview
additional information
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Knockdown of NbSACPD-C expression via VIGS causes seedlessness in Nicotiana benthamiana and alters lipids profiles in the ovary. Phenotypes, overview
additional information
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Knockdown of NbSACPD-C expression via VIGS causes seedlessness in Nicotiana benthamiana and alters lipids profiles in the ovary. Phenotypes, overview
additional information
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Knockdown of NbSACPD-C expression via VIGS causes seedlessness in Nicotiana benthamiana and alters lipids profiles in the ovary. Phenotypes, overview
additional information
-
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Knockdown of NbSACPD-C expression via VIGS causes seedlessness in Nicotiana benthamiana and alters lipids profiles in the ovary. Phenotypes, overview
additional information
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Phenotypes, overview
additional information
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Phenotypes, overview
additional information
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Phenotypes, overview
additional information
-
knockdown of the expression of individual or combinations of NbSACPD isozymes by an artificial microRNA approach results in significantly reduced accumulation of 18C unsaturated fatty acids and elevated levels of 18:0-fatty acid in leaves, indicating that all three genes A-C participate in fatty acid desaturation. Phenotypes, overview
additional information
XsSAD expression in the Arabidopsis thaliana ssi2 mutant partially complements the morphological phenotype of Arabidopsis thaliana ssi2 plants. Fatty acid composition of wild-type and mutant plants, overview
additional information
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XsSAD expression in the Arabidopsis thaliana ssi2 mutant partially complements the morphological phenotype of Arabidopsis thaliana ssi2 plants. Fatty acid composition of wild-type and mutant plants, overview
additional information
determination of single nucleotide polymorphisms (SNPs) and genotyping of SAD1, ZmSAD1-based association mapping, overview. Associations of SNPs in 11 SAD genes with stearic, oleic acid and their ratio
additional information
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determination of single nucleotide polymorphisms (SNPs) and genotyping of SAD1, ZmSAD1-based association mapping, overview. Associations of SNPs in 11 SAD genes with stearic, oleic acid and their ratio
additional information
ZmSAD1 overexpression (ZmSAD1), antisense ZmSAD1 (anti-ZmSAD1), and ZmSAD1 RNA interference (ZmSAD1 RNAi) constructs are generated in the pBI121 vector under control of the seed-specific FAE1 promoter. Ttransformation of the ZmSAD1 and ZmSAD1 RNAi constructs under control ofthe FAE1 promoter into maize by particle bombardment. Overexpression of ZmSAD1 changes the fatty acid composition in maize seeds, expression of seed-specific ZmSAD1 results in a decrease in the content of stearic and other saturated acids. Composition of fatty acids in the maize seeds harbouring ZmSAD1 or ZmSAD1 RNAi constructs, phenotypes, overview
additional information
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ZmSAD1 overexpression (ZmSAD1), antisense ZmSAD1 (anti-ZmSAD1), and ZmSAD1 RNA interference (ZmSAD1 RNAi) constructs are generated in the pBI121 vector under control of the seed-specific FAE1 promoter. Ttransformation of the ZmSAD1 and ZmSAD1 RNAi constructs under control ofthe FAE1 promoter into maize by particle bombardment. Overexpression of ZmSAD1 changes the fatty acid composition in maize seeds, expression of seed-specific ZmSAD1 results in a decrease in the content of stearic and other saturated acids. Composition of fatty acids in the maize seeds harbouring ZmSAD1 or ZmSAD1 RNAi constructs, phenotypes, overview
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a Brassica napus bacterial artificial chromosome, BAC, library is constructed
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cloned in pQE-30 vector and expressed in Escherichia coli strain M15, His-tag
DesA1 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid
DesA2 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression of 2 isoforms in Escherichia coli
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expression of cDNA in Escherichia coli
gene CocoFAD, isolated from a cDNA library prepared from the endosperm of coconut, DNA and amino acid sequence determmination and analysis, sequence comparisons and phylogenetic analysis and tree, real-time fluorescent quantitative PCR expresion analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1, the levels of palmitoleic acid (16:1) and oleic acid (18:1) are improved significantly, stearic acid (18:0) is reduced in recombinant cells
gene FAB2, real-time quantitative PCR enzyme expression analysis
gene FAB2, sequence comparisons, semi-quantitative RT-PCR expression analysis, recombinant overexpression in Chlamydomonas reinhardtii
gene Gmsacpd-c, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
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gene NbSACPD-A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene NbSACPD-B, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene NbSACPD-C, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene sad, cloning from embryo, DNA and amino acid sequence determination and analysis, quantitative and semi-quantitative real-time RT-PCR enzyme expression analysis, recombinant XsSAD expression in Escherichia coli cells resulting in increased 18:1D9 level, and confirming the biological activity of the enzyme encoded by XsSAD. Recombinant XsSAD expression in and partial complementation of Arabidopsis thaliana ssi2 mutant, via Agrobacterium strain GV3101 transformation method,control of 35S promoter partially restors the morphological phenotype and effectively increases the 18:1D9 level. The levels of other unsaturated fatty acids synthesized with 18:1D9 as the substrate also increase to some degree
gene SAD, DNA and amino acid sequence determination and analysis, enzyme expressioon analysis
gene SAD, DNA and amino acid sequence determination and analysis, ZmSAD1-based association mapping, genotyping, sequence comparisons, phylogenetic analysis, quantitative reverse transcription PCR enzyme expression analysis
gene sad, multiple copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
gene SAD1, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD1, cloning of the isozyme from Olea europaea cv. Picual, DNA and amino acid sequence determination and analysis, isozyme sequence comparison and phylogenetic analysis, expression analysis
gene SAD1, phylogenetic analysis
gene SAD1, phylogenetic analysis, ZmSAD1 overexpression (ZmSAD1), antisense ZmSAD1 (anti-ZmSAD1), and ZmSAD1 RNA interference (ZmSAD1 RNAi) constructs are generated in the pBI121 vector under control of the seed-specific FAE1 promoter, recombinant expression in Arabidopsis thaliana with higher expression levels in mature seeds and immature siliques than in roots, stems, leaves, and petals. Fatty acid composition and content are modified in the transgenic Arabidopsis seeds, seed-specific overexpression of the exogenous ZmSAD1 gene significantly reduces the content of stearic acid and the ratio of saturated to unsaturated fatty acids, composition of fatty acids in the Arabidopsis seeds harbouring the exogenous ZmSAD1, anti-ZmSAD1, or ZmSAD1 RNAi constructs. Phenotypes, overview
gene SAD2, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD2, cloning of the isozyme from Olea europaea cv. Picual, DNA and amino acid sequence determination and analysis, isozyme sequence comparison and phylogenetic analysis, expression analysis
gene SAD3, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD3, cloning of the isozyme from Olea europaea cv. Picual, DNA and amino acid sequence determination and analysis, isozyme sequence comparison and phylogenetic analysis, expression analysis
gene SAD4, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD5, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD6, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD7, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, SAD7 cannot be recombinantly expressed in Arabidopsis thaliana
gene SAD8, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis
into the pUCm-T vector and subcloned into the vector pET30a for expression in Escherichia coli BL21DE3 cells
into the vector pQE-30 for expression in Escherichia coli M15 cells
OeSAD2 is not expressed as a soluble protein
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S-ACP-DES1 expressed in Escherichia coli
S-ACP-DES3 enzyme expressed in Escherichia coli
S-ACP-DES4 enzyme expressed in Escherichia coli
S-ACP-DES5 enzyme expressed in Escherichia coli
expression in Escherichia coli
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expression in Escherichia coli
Bignonia unguis-cati
expression of cDNA in Escherichia coli
expression of cDNA in Escherichia coli
-
expression of cDNA in Escherichia coli
-
expression of cDNA in Escherichia coli
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Jaworski, J.G.; Stumpf, P.K.
Fat metabolism in higher plants. Properties of a soluble stearyl-acyl carrier protein desaturase from maturing Carthamus tinctorius
Arch. Biochem. Biophys.
162
158-165
1974
Carthamus tinctorius (P22243), Carthamus tinctorius
brenda
Nagai, J.; Bloch, K.
Enzymatic desaturation of stearyl acyl carrier protein
J. Biol. Chem.
243
4626-4633
1968
Euglena gracilis
brenda
Knutzon, D.S.; Scherer, D.E.; Schreckengost, W.E.
Nucleotide sequence of a complementary DNA clone encoding stearoyl-acyl carrier protein desaturase from castor bean, Ricinus communis
Plant Physiol.
96
344-345
1991
Ricinus communis (P22337), Ricinus communis
brenda
Shanklin, J.; Somerville, C.
Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs
Proc. Natl. Acad. Sci. USA
88
2510-2514
1991
Persea americana, Cucumis sativus, Ricinus communis (P22337), Ricinus communis
brenda
Thompson, G.A.; Scherer, D.E.; Foxall-van Aken, S.; Kenny, J.W.; Young, H.L.; Shitani, D.K.; Kridl, J.C.; Knauf, V.C.
Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity
Proc. Natl. Acad. Sci. USA
88
2578-2582
1991
Carthamus tinctorius (P22243), Carthamus tinctorius
brenda
McKeon, T.A.; Stumpf, P.K.
Purification and characterization of the stearoyl-acyl carrier protein desaturase and the acyl-acyl carrier protein thioesterase from maturing seeds of safflower
J. Biol. Chem.
257
12141-12147
1982
Carthamus tinctorius (P22243), Carthamus tinctorius
brenda
Schneider, G.; Lindquist, Y.; Shanklin, J.; Somerville, C.
Preliminary crystallographic data for stearoyl-acyl carrier protein desaturase from castor seed
J. Mol. Biol.
225
561-564
1992
Ricinus communis (P22337)
brenda
McKeon, T.A.; Stumpf, P.K.
Stearoyl-acyl carrier protein desaturase from safflower seeds
Methods Enzymol.
71
275-281
1981
Carthamus tinctorius (P22243)
-
brenda
Nagai, J.; Bloch, K.
Enzymatic desaturation of stearyl acyl carrier protein
J. Biol. Chem.
241
1925-1927
1966
Euglena gracilis, Spinacia oleracea
brenda
Stumpf, P.K.; Porra, R.J.
Lipid biosynthesis in developing and germinating soybean cotyledons. The formation of oleate by a soluble stearyl acyl carrier protein desaturase
Arch. Biochem. Biophys.
176
63-70
1976
Glycine max
brenda
Fox, B.G.; Shanklin, J.; Somerville, C.; Munck, E.
Stearoyl-acyl carrier protein DELTA9 desaturase from Ricinus communis is a diiron-oxo protein
Proc. Natl. Acad. Sci. USA
90
2486-2490
1993
Ricinus communis (P22337), Ricinus communis
brenda
Schultz, D.; Cahoon, E.B.; Shanklin, J.; Craig, R.; Cox-Foster, D.L.; Mumma, R.O.; Medford, J.I.
Expression of a DELTA9 14:0-acyl carrier protein fatty acid desaturase gene is necessary for the production of w5 anacardic acids found in pest-resistant geranium (Pelargonium xhortorum)
Proc. Natl. Acad. Sci. USA
93
8771-8775
1996
Pelargonium x hortorum
brenda
Cahoon, E.B.; Lindqvist, Y.; Schneider, G.; Shanklin, J.
Redesign of soluble fatty acid desaturases from plants from altered substrate specificity and double bond position
Proc. Natl. Acad. Sci. USA
94
4872-4877
1997
Ricinus communis (P22337)
brenda
Cahoon, E.B.; Coughlan, S.J.; Shanklin, J.
Characterization of a structurally and functionally diverged acyl-acyl carrier protein desaturase from milkweed seed
Plant Mol. Biol.
33
1105-1110
1997
Asclepias syriaca
brenda
Cahoon, E.B.; Shah, S.; Shanklin, J.; Browse, J.
A determinant of substrate specificity predicted from the acyl-acyl carrier protein desaturase of developing cat's claw seed
Plant Physiol.
117
593-598
1998
Bignonia unguis-cati (O65040), Ricinus communis (P22337)
brenda
Broadwater, J.A.; Ai, J.; Loehr, T.M.; Sanders-Loehr, J.; Fox, B.G.
Peroxodiferric intermediate of stearoyl-acyl carrier protein DELTA9 desaturase: oxidase reactivity during single turnover and implications for the mechanism of desaturation
Biochemistry
37
14664-14671
1998
Ricinus communis (P22337)
brenda
Haas, J.A.; Fox, B.G.
Role of hydrophobic partitioning in substrate selectivity and turnover of the ricinus communis stearoyl acyl carrier protein DELTA9 desaturase
Biochemistry
38
12833-12840
1999
Ricinus communis (P22337)
brenda
Gummeson, P.O.; Lenman, M.; Lee, M.; Singh, S.; Stymne, S.
Characterization of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L
Plant Sci.
154
53-60
2000
Macadamia integrifolia, Nerium oleander
brenda
Shah, F.H.; Rashid, O.; San, C.T.
Temporal regulation of two isoforms of cDNA clones encoding delta 9-stearoyl-ACP desaturase from oil palm (Elaeis guineensis)
Plant Sci.
152
27-33
2000
Elaeis guineensis, Elaeis guineensis (Q9XFH1)
-
brenda
Schultz, D.J.; Suh, M.C.; Ohlrogge, J.B.
Stearoyl-acyl carrier protein and unusual acyl-acyl carrier protein desaturase activities are differentially influenced by ferredoxin
Plant Physiol.
124
681-692
2000
Coriandrum sativum, Pelargonium x hortorum, Spinacia oleracea
brenda
Whitney, H.; Sayanova, O.; Lewis, M.J.; Pickett, J.; Napier, J.A.
Isolation of two putative acyl-acyl carrier protein desaturase enzymes from Kochia scoparia
Biochem. Soc. Trans.
28
623-624
2000
Bassia scoparia
brenda
Whittle, E.; Shanklin, J.
Engineering D9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor DELTA9-18:0-ACP desaturase
J. Biol. Chem.
276
21500-21505
2001
Ricinus communis (P22337)
brenda
Rogge, C.E.; Fox, B.G.
Desaturation, chain scission, and register-shift of oxygen-substituted fatty acids during reaction with stearoyl-ACP desaturase
Biochemistry
41
10141-10148
2002
Ricinus communis (P22337)
brenda
Davydov, R.; Behrouzian, B.; Smoukov, S.; Stubbe, J.; Hoffman, B.M.; Shanklin, J.
Effect of substrate on the diiron(III) site in stearoyl acyl carrier protein DELTA9-desaturase as disclosed by cryoreduction electron paramagnetic resonance/electron nuclear double resonance spectroscopy
Biochemistry
44
1309-1315
2005
Ricinus communis
brenda
Moche, M.; Shanklin, J.; Ghoshal, A.; Lindqvist, Y.
Azide and acetate complexes plus two iron-depleted crystal structures of the di-iron enzyme DELTA9 stearoyl-acyl carrier protein desaturase: implications for oxygen activation and catalytic intermediates
J. Biol. Chem.
278
25072-25080
2003
Ricinus communis (P22337), Ricinus communis
brenda
Serrano-Vega, M.J.; Venegas-Caleron, M.; Garces, R.; Martinez-Force, E.
Cloning and expression of fatty acids biosynthesis key enzymes from sunflower (Helianthus annuus L.) in Escherichia coli
J. Chromatogr. B
786
221-228
2003
Helianthus annuus (O24498)
brenda
Salas, J.J.; Martinez-Force, E.; Garces, R.
Biochemical characterization of a high-palmitoleic acid Helianthus annuus mutant
Plant Physiol. Biochem.
42
373-381
2004
Helianthus annuus
brenda
Luo, T.; Peng, S.; Deng, W.; Ma, D.; Xu, Y.; Xiao, M.; Chen, F.
Characterization of a New Stearoyl-acyl Carrier Protein Desaturase Gene from Jatropha curcas
Biotechnol. Lett.
28
657-662
2006
Jatropha curcas (Q4JIJ4), Jatropha curcas
brenda
Fofana, B.; Cloutier, S.; Duguid, S.; Ching, J.; Rampitsch, C.
Gene expression of stearoyl-ACP desaturase and delta12 fatty acid desaturase 2 is modulated during seed development of flax (Linum usitatissimum)
Lipids
41
705-712
2006
Linum usitatissimum
brenda
Kachroo, A.; Shanklin, J.; Whittle, E.; Lapchyk, L.; Hildebrand, D.; Kachroo, P.
The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis
Plant Mol. Biol.
63
257-271
2007
Arabidopsis thaliana (O22832), Arabidopsis thaliana (Q84VY3), Arabidopsis thaliana (Q9LF04), Arabidopsis thaliana (Q9LF05), Arabidopsis thaliana (Q9M879), Arabidopsis thaliana (Q9M880), Arabidopsis thaliana (Q9M881)
brenda
Dyer, D.H.; Lyle, K.S.; Rayment, I.; Fox, B.G.
X-ray structure of putative acyl-ACP desaturase DesA2 from Mycobacterium tuberculosis H37Rv
Protein Sci.
14
1508-1517
2005
Mycobacterium tuberculosis (P9WNZ5), Mycobacterium tuberculosis (P9WNZ7), Mycobacterium tuberculosis H37Rv (P9WNZ5), Mycobacterium tuberculosis H37Rv (P9WNZ7), Mycobacterium tuberculosis H37Rv
brenda
Cho, K.; ONeill, C.M.; Kwon, S.J.; Yang, T.J.; Smooker, A.M.; Fraser, F.; Bancroft, I.
Sequence-level comparative analysis of the Brassica napus genome around two stearoyl-ACP desaturase loci
Plant J.
61
591-599
2009
Brassica napus
brenda
Luo, T.; Deng, W.; Zeng, J.; Zhang, F.
Cloning and characterization of a stearoyl-acyl carrier protein desaturase gene from Cinnamomum longepaniculatum
Plant Mol. Biol. Rep.
27
13-19
2009
Cinnamomum longipaniculatum (A8D2K7)
-
brenda
Cao, Y.; Xian, M.; Yang, J.; Xu, X.; Liu, W.; Li, L.
Heterologous expression of stearoyl-acyl carrier protein desaturase (S-ACP-DES) from Arabidopsis thaliana in Escherichia coli
Protein Expr. Purif.
69
209-214
2010
Arabidopsis thaliana (O22832), Arabidopsis thaliana
brenda
Shilman, F.; Brand, Y.; Brand, A.; Hedvat, I.; Hovav, R.
Identification and Molecular Characterization of Homeologous ?9-Stearoyl Acyl Carrier Protein Desaturase 3 Genes from the Allotetraploid Peanut (Arachis hypogaea)
Plant Mol. Biol. Rep.
29
232-241
2011
Arachis hypogaea
-
brenda
Schlueter, P.M.; Xu, S.; Gagliardini, V.; Whittle, E.; Shanklin, J.; Grossniklaus, U.; Schiestl, F.P.
Stearoyl-acyl carrier protein desaturases are associated with floral isolation in sexually deceptive orchids
Proc. Natl. Acad. Sci. USA
108
5696-5701
2011
Ophrys sphegodes, Ophrys sphegodes subsp. sphegodes
brenda
Hwangbo, K.; Ahn, J.; Lim, J.; Park, Y.; Liu, J.; Jeong, W.
Overexpression of stearoyl-ACP desaturase enhances accumulations of oleic acid in the green alga Chlamydomonas reinhardtii
Plant Biotechnol. Rep.
8
135-142
2014
Chlamydomonas reinhardtii (A8IQB8)
-
brenda
Shelley, I.J.; Nishiuchi, S.; Shibata, K.; Inukai, Y.
SLL1, which encodes a member of the stearoyl-acyl carrier protein fatty acid desaturase family, is involved in cell elongation in lateral roots via regulation of fatty acid content in rice
Plant Sci.
207
12-17
2013
Oryza sativa
brenda
Liu, J.; Sun, Z.; Zhong, Y.; Huang, J.; Hu, Q.; Chen, F.
Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis
Planta
236
1665-1676
2012
Chromochloris zofingiensis (D0V0B8), Chromochloris zofingiensis, Chromochloris zofingiensis ATCC 30412 (D0V0B8)
brenda
Ruddle, P.; Whetten, R.; Cardinal, A.; Upchurch, R.G.; Miranda, L.
Effect of DELTA9-stearoyl-ACP-desaturase-C mutants in a high oleic background on soybean seed oil composition
Theor. Appl. Genet.
127
349-358
2014
Glycine max
brenda
Scaglia, B.; Cassani, E.; Pilu, R.; Adani, F.
Expression of Arabidopsis thaliana S-ACP-DES3 in Escherichia coli for high-performance biodiesel production
RSC Adv.
4
63387-63392
2014
Arabidopsis thaliana (Q9LF05)
-
brenda
de Jaeger, L.; Springer, J.; Wolbert, E.J.H.; Martens, D.E.; Eggink, G.; Wijffels, R.H.
Gene silencing of stearoyl-ACP desaturase enhances the stearic acid content in Chlamydomonas reinhardtii
Biores. Technol.
245
1616-1626
2017
Chlamydomonas reinhardtii (A8IQB8), Chlamydomonas reinhardtii, Chlamydomonas reinhardtii 330 (A8IQB8)
brenda
Du, H.; Huang, M.; Hu, J.; Li, J.
Modification of the fatty acid composition in Arabidopsis and maize seeds using a stearoyl-acyl carrier protein desaturase-1 (ZmSAD1) gene
BMC Plant Biol.
16
137
2016
Zea mays (B4F9Q1), Zea mays, Arabidopsis thaliana (Q9LF04)
brenda
Zhang, Y.; Maximova, S.N.; Guiltinan, M.J.
Characterization of a stearoyl-acyl carrier protein desaturase gene family from chocolate tree, Theobroma cacao L
Front. Plant Sci.
6
239
2015
Theobroma cacao (A0A0F6SYT9), Theobroma cacao (A0A0F6SYU0), Theobroma cacao (A0A0F6X2R5), Theobroma cacao (A0A0F6X2V1), Theobroma cacao (A0A0F6ZN15), Theobroma cacao (A0A0F6ZN93), Theobroma cacao (A0A0F6ZNE1), Theobroma cacao (A0A0F6ZQT3), Theobroma cacao
brenda
Ramesh, A.M.; Kesari, V.; Rangan, L.
Characterization of a stearoyl-acyl carrier protein desaturase gene from potential biofuel plant, Pongamia pinnata L.
Gene
542
113-121
2014
Pongamia pinnata (S5U3F4), Pongamia pinnata
brenda
Gao, L.; Sun, R.; Liang, Y.; Zhang, M.; Zheng, Y.; Li, D.
Cloning and functional expression of a cDNA encoding stearoyl-ACP ?9-desaturase from the endosperm of coconut (Cocos nucifera L.)
Gene
549
70-76
2014
Cocos nucifera (A0A0C4MIW7)
brenda
Parvini, F.; Sicardo, M.; Hosseini-Mazinani, M.; Martinez-Rivas, J.; Hernandez, M.
Transcriptional analysis of stearoyl-acyl carrier protein desaturase genes from olive (Olea europaea) in relation to the oleic acid content of the virgin olive oil
J. Agric. Food Chem.
64
7770-7781
2016
Olea europaea (A0A0D4D8J5), Olea europaea (A0A1D6ZNQ5), Olea europaea (A0A1D6ZNR4)
brenda
Hwangbo, K.; Ahn, J.; Lim, J.; Park, Y.; Liu, J.; Jeong, W.
Overexpression of stearoyl-ACP desaturase enhances accumulations of oleic acid in the green alga Chlamydomonas reinhardtii
Plant Biotechnol. Rep.
8
135-142
2014
Chlamydomonas reinhardtii (A8IQB8)
-
brenda
Zhang, J.; Li, J.; Garcia-Ruiz, H.; Bates, P.D.; Mirkov, T.E.; Wang, X.
A stearoyl-acyl carrier protein desaturase, NbSACPD-C, is critical for ovule development in Nicotiana benthamiana
Plant J.
80
489-502
2014
Nicotiana benthamiana (A0A060IKL1), Nicotiana benthamiana (A0A060INU6), Nicotiana benthamiana (A0A060IVB8), Nicotiana benthamiana
brenda
Dunn, Y.; La, H.; Huang, M.; Zhao, F.; Teug, J.; Zhang, A.; Sheng, W.; Zhu, Y.; Xue, J.
Cloning and in silico analysis of two genes, stearoyl-ACP desaturase (PtSAD) and small heat shock protein (PtsHSP), in response to heat stress of Pinellia ternata
Plant OMICS
8
316-321
2015
Pinellia ternata (I3RQI7)
-
brenda
Lakhssassi, N.; Colantonio, V.; Flowers, N.D.; Zhou, Z.; Henry, J.; Liu, S.; Meksem, K.
Stearoyl-acyl carrier protein desaturase mutations uncover an impact of stearic acid in leaf and nodule structure
Plant Physiol.
174
1531-1543
2017
Glycine max
brenda
Zhao, N.; Zhang, Y.; Li, Q.; Li, R.; Xia, X.; Qin, X.; Guo, H.
Identification and expression of a stearoyl-ACP desaturase gene responsible for oleic acid accumulation in Xanthoceras sorbifolia seeds
Plant Physiol. Biochem.
87
9-16
2015
Xanthoceras sorbifolium (A0A0K0MC84), Xanthoceras sorbifolium
brenda
Han, Y.; Xu, G.; Du, H.; Hu, J.; Liu, Z.; Li, H.; Li, J.; Yang, X.
Natural variations in stearoyl-acp desaturase genes affect the conversion of stearic to oleic acid in maize kernel
Theor. Appl. Genet.
130
151-161
2017
Zea mays (B4F9Q1), Zea mays
brenda