Information on EC 1.14.19.11 - acyl-[acyl-carrier-protein] 4-desaturase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.14.19.11
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RECOMMENDED NAME
GeneOntology No.
acyl-[acyl-carrier-protein] 4-desaturase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = (4Z)-hexadec-4-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
petroselinate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
palmitoyl-[acyl-carrier protein],reduced acceptor:oxygen oxidoreductase (4,5 cis-dehydrogenating)
The enzymes from the plants Coriandrum sativum (coriander) and Hedera helix (English ivy) are involved in biosynthesis of petroselinate [(6Z)-octadec-6-enoate], which is formed by elongation of (4Z)-hexadec-4-enoate. The ivy enzyme can also act on oleoyl-[acyl-carrier protein] and palmitoleoyl-[acyl-carrier protein], generating the corresponding 4,9-diene.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
English ivy
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
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-
-
-
?
palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
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-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
?
show the reaction diagram
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desaturation occurs at the DELTA9 position
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-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
oleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
(4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O
show the reaction diagram
stearoyl-[acyl-carrier protein] + reduced acceptor + O2
?
show the reaction diagram
-
desaturation occurs at the DELTA9 position
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-
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
oleoyl-[acyl-carrier protein]
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pH and temperature not specified in the publication
0.0021
palmitoleoyl-[acyl-carrier protein]
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pH and temperature not specified in the publication
0.0015
palmitoyl-[acyl-carrier protein]
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pH and temperature not specified in the publication
0.0015
stearoyl-[acyl-carrier protein]
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pH and temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
oleoyl-[acyl-carrier protein]
Hedera helix
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pH and temperature not specified in the publication
0.048
palmitoleoyl-[acyl-carrier protein]
Hedera helix
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pH and temperature not specified in the publication
0.25
palmitoyl-[acyl-carrier protein]
Hedera helix
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pH and temperature not specified in the publication
0.17
stearoyl-[acyl-carrier protein]
Hedera helix
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pH and temperature not specified in the publication
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
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x * 36000, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 36000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Poros 20 CM column chromatography and G-300SW gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana
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expressed in Escherichia coli BL21 cells and leaf discs of Nicotiana tabacum
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expressed in Escherichia coli BL21 DE3 Gold cells and in Arabidopsis thaliana
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expressed in Nicotiana tabacum cells
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.19.11)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)