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EC Tree
The expected taxonomic range for this enzyme is: Berberis stolonifera
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(S)-N-methylcoclaurine + ascorbate + O2 = (S)-3'-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
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(S)-N-methylcoclaurine,ascorbate:oxygen oxidoreductase (3'-hydroxylating)
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(R)-coclaurine + ascorbate + O2
(R)-3-hydroxycoclaurine + dehydroascorbate + H2O
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(S)-coclaurine + ascorbate + O2
(S)-3-hydroxycoclaurine + dehydroascorbate + H2O
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4-methylcatechol + ascorbate + O2
4-methyl-o-benzoquinone + dehydroascorbate + H2O
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L-tyrosine + ascorbate + O2
L-dopa + dehydroascorbate + H2O
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N-methylcoclaurine + ascorbate + O2
3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O
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tyramine + ascorbate + O2
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O
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azide
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12 mM, 50% inhibition
CN-
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1 mM, complete inhibition
diethyldithiocarbamate
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0.3 mM, complete inhibition
EDTA
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no inhibition EDTA
Thiourea
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13 mM, 50% inhibition
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4.5 - 7.5
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50% of maximal activity at pH 4.5 and pH 7.5
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brenda
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36000
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2 * 36000, SDS-PAGE
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dimer
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2 * 36000, SDS-PAGE
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Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing
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Loeffler, S.; Zenk, M.H.
The hydroxylation step in the biosynthetic pathway leading from norcolaurine to reticuline
Phytochemistry
29
3499-3503
1990
Berberis stolonifera
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brenda
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