Information on EC 1.14.15.11 - pentalenic acid synthase

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The expected taxonomic range for this enzyme is: Streptomyces avermitilis

EC NUMBER
COMMENTARY
1.14.15.11
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RECOMMENDED NAME
GeneOntology No.
pentalenic acid synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1-deoxypentalenate + reduced ferredoxin + O2 = pentalenate + oxidized ferredoxin + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
neopentalenoketolactone and pentalenate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
1-deoxypentalenate,reduced ferredoxin:O2 oxidoreductase
A heme-thiolate enzyme (P-450). Isolated from the bacterium Streptomyces avermitilis. The product, pentalenate, is a co-metabolite from pentalenolactone biosynthesis.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CYP105D7
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CYP105D7
Streptomyces avermitilis MA4680
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sav7469
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gene name
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SAV_7469
locus name
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Streptomyces avermitilis MA4680
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
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disruption of the sav7469 gene encoding pentalenic acid synthase abolishes production of pentalenic acid. The deletion mutant accumulates 1-deoxypentalenic acid. Recombinant enzyme prepared from Escherichia coli catalyzes the oxidative conversion of 1-deoxypentalenic acid to pentalenic acid in the presence of the electron-transport partners, ferredoxin and ferredoxin reductase, both in vivo and in vitro
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-deoxypentalenic acid + reduced ferredoxin + O2
pentalenic acid + oxidized ferredoxin + H2O
show the reaction diagram
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?
additional information
?
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enzyme additionally catalyzes the hydroxylation of daidzein to 7,3',4'-trihydroxyisoflavone with apparent Km and kcat values for daidzein of 21.83 mM and 15.01 per min in the presence of putidaredoxin and putidaredoxin reductase, respectively
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additional information
?
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no substrates: pentalenene, 1-deoxy-11beta-hydroxypentalenic acid, 1-deoxy-11-oxopentalenic acid, or the corresponding methyl esters of 1-deoxy-11beta-hydroxypentalenic acid, 1-deoxy-11-oxopentalenic acid and 1-deoxypentalenic acid, respectively
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additional information
?
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Streptomyces avermitilis MA4680
enzyme additionally catalyzes the hydroxylation of daidzein to 7,3',4'-trihydroxyisoflavone with apparent Km and kcat values for daidzein of 21.83 mM and 15.01 per min in the presence of putidaredoxin and putidaredoxin reductase, respectively
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.027
1-deoxypentalenic acid
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pH 8.0, 25°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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