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IUBMB CommentsA cytochrome P-450 (heme-thiolate) protein. Isolated from Oryza sativa (rice). Oryzalexin D is a phytoalexin.
The expected taxonomic range for this enzyme is: Oryza sativa
Synonyms
cyp76m8,
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EC 1.14.13.193
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formerly
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CYP76M8
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ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2 = oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
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ent-sandaracopimaradien-3beta-ol,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (oryzalexin D forming)
A cytochrome P-450 (heme-thiolate) protein. Isolated from Oryza sativa (rice). Oryzalexin D is a phytoalexin.
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ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
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ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
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ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
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ent-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
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ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
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syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
momilactone A + [oxidized NADPH-hemoprotein reductase] + H2O
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additional information
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no reaction with syn-pimaradien-19-oic acid, syn-stemodene, syn-stemarene, or syn-labdatriene
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ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
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0.004
ent-cassadiene
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pH and temperature not specified in the publication
0.015
ent-isokaurene
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pH and temperature not specified in the publication
0.005
ent-kaurene
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pH and temperature not specified in the publication
0.004
ent-pimaradiene
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pH and temperature not specified in the publication
0.007
ent-sandaracopimaradien-3beta-ol
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pH and temperature not specified in the publication
0.002
syn-pimaradiene
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pH and temperature not specified in the publication
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0.006
ent-cassadiene
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pH and temperature not specified in the publication
0.015
ent-isokaurene
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pH and temperature not specified in the publication
0.009
ent-kaurene
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pH and temperature not specified in the publication
0.009
ent-pimaradiene
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pH and temperature not specified in the publication
0.011
ent-sandaracopimaradien-3beta-ol
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pH and temperature not specified in the publication
0.006
syn-pimaradiene
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pH and temperature not specified in the publication
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1.5
ent-cassadiene
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pH and temperature not specified in the publication
1
ent-isokaurene
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pH and temperature not specified in the publication
1.8
ent-kaurene
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pH and temperature not specified in the publication
2.3
ent-pimaradiene
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pH and temperature not specified in the publication
1.6
ent-sandaracopimaradien-3beta-ol
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pH and temperature not specified in the publication
2.6
syn-pimaradiene
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pH and temperature not specified in the publication
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brenda
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C76M8_ORYSJ
500
1
55933
Swiss-Prot
Mitochondrion (Reliability: 3)
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expressed in Escherichia coli C41 cells
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Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.
Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster
J. Biol. Chem.
287
6159-6168
2012
Oryza sativa
brenda
Wu, Y.; Wang, Q.; Hillwig, M.; Peters, R.
Picking sides: Distinct roles for CYP76M6 and CYP76M8 in rice oryzalexin biosynthesis
Biochem. J.
454
209-216
2013
Oryza sativa
brenda
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