Information on EC 1.14.13.91 - deoxysarpagine hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)
Please login to have access to the AMENDA and FRENDA data


The expected taxonomic range for this enzyme is: Rauvolfia serpentina

top print hide
EC NUMBER
COMMENTARY hide
1.14.13.91
-
top print hide
RECOMMENDED NAME
GeneOntology No.
deoxysarpagine hydroxylase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
10-deoxysarpagine + NADPH + H+ + O2 = sarpagine + NADP+ + H2O
show the reaction diagram
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ajmaline and sarpagine biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Indole alkaloid biosynthesis
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
10-deoxysarpagine,NADPH:oxygen oxidoreductase (10-hydroxylating)
A heme-thiolate protein (P-450).
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10-deoxysarpagine + NADPH + O2
sarpagine + NADP+ + H2O
show the reaction diagram
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-deoxysarpagine + NADPH + O2
sarpagine + NADP+ + H2O
show the reaction diagram
-
most probably the natural substrate
-
-
?
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
can not be replaced by NADH
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ancymidole
-
1.0 mM, 49% inhibition
BAS 110 W
-
1 mM, complete inhibition
BAS 111 W
-
1 mM, 84% inhibition
CO
-
ratio CO:O2 of 9:1, 70% inhibition in the light, 80% inhibition under red light, 77% inhibition under blue light
cytochrome c
-
0.01 mM, 46% inhibition
ketoconazole
-
1 mM, 69% inhibition
LAB 1500978
-
1 mM, 74% inhibition
Metyrapone
-
1 mM, 83% inhibition
NADP+
-
competitive inhibition
Tetcyclacis
-
1 mM, 45% inhibition
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0074
10-deoxysarpagine
-
35°C, PH 8.0
0.025
NADPH
-
35°C, PH 8.0
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-25°C, 20% sucrose, more than 3 months, no loss of activity
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.91)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)