Information on EC 1.14.13.89 - isoflavone 2'-hydroxylase

Word Map on EC 1.14.13.89
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.89
-
RECOMMENDED NAME
GeneOntology No.
isoflavone 2'-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an isoflavone + NADPH + H+ + O2 = a 2'-hydroxyisoflavone + NADP+ + H2O
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Isoflavonoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
(-)-glycinol biosynthesis
-
-
coumestrol biosynthesis
-
-
wighteone and luteone biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
isoflavone,NADPH:oxygen oxidoreductase (2'-hydroxylating)
A heme-thiolate protein (P-450). Acts on daidzein, formononetin and genistein. EC 1.14.13.53, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
CAS REGISTRY NUMBER
COMMENTARY hide
110183-49-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-hydroxyformononetin + NADPH + O2
?
show the reaction diagram
9.1% of the activity with biochanin A
-
-
?
3'-hydroxyformononetin + NADPH + O2
?
show the reaction diagram
4.7% of the activity with biochanin A
-
-
?
biochanin A + NADPH + O2
2'-hydroxybiochanin A + NADP+ + H2O
show the reaction diagram
-
-
-
?
daidzein + NADPH + O2
2'-hydroxydaidzein + NADP+ + H2O
show the reaction diagram
formononetin + NADPH + O2
2'-hydroxyformononetin + NADP+ + H2O
show the reaction diagram
genistein + NADPH + O2
2'-hydroxygenistein + NADP+ + H2O
show the reaction diagram
pseudobaptigenin + NADPH + O2
?
show the reaction diagram
35% of the activity with biochanin A
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
NADPH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.051
Biochanin A
pH 8.0
0.067
formononetin
pH 8.0
0.00017
NADPH
pH 8.0
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
Biochanin A
pH 8.0
0.015
formononetin
pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
suspension, induction with yeast extract
Manually annotated by BRENDA team
-
activity increases 10 h after elicitation with reduced glutathione. Only a low level of transcription is observed in untreated seedlings
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
expression in yeast
-
expression in yeast strain WAT11