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Information on EC 1.14.13.81 - magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase and Organism(s) Chlamydomonas reinhardtii and UniProt Accession Q9LD46

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IUBMB Comments
Requires Fe(II) for activity. The enzyme participates in the biosynthesis of chlorophyllide a in aerobic organisms. The same transformation is achieved in anaerobic organisms by EC 1.21.98.3, anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase. Some facultative phototrophic bacteria, such as Rubrivivax gelatinosus, possess both enzymes.
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This record set is specific for:
Chlamydomonas reinhardtii
UNIPROT: Q9LD46
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The taxonomic range for the selected organisms is: Chlamydomonas reinhardtii
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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magnesium-protoporphyrin IX 13-monomethyl ester
+
3
NADPH
+
3
H+
+
3
O2
=
3,8-divinyl protochlorophyllide a
+
3
NADP+
+
5
H2O
+
3
+
3
+
3
=
+
3
+
5
Synonyms
chl27, mg-protoporphyrin ix monomethyl ester cyclase, xantha-l, pempec, mg-protoporphyrin ix monomethyl ester (oxidative) cyclase, sll1214, mg protoporphyrin monomethylester cyclase, oscrd1, mpe-cyclase, magnesium-protoporphyrin ix monomethyl ester cyclase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crd1 protein
Chlamydomonas reinhardtii, first identified as gene in response to copper deficiency, one of two isozymes
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Cth1 protein
copper target homolog 1, isoenzyme to Crd1
cyclase, magnesium protoporphyrin IX monomethyl ester
-
-
-
-
magnesium-protoporphyrin IX monomethyl ester cyclase
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magnesium-protoporphyrin-IX monomethyl ester cyclase
-
-
-
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magnesium-protoporphyrin-IX monomethyl ester oxidative cyclase
-
-
-
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Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
-
-
-
-
redox reaction
-
-
-
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oxidation
-
-
-
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reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen oxidoreductase (hydroxylating)
Requires Fe(II) for activity. The enzyme participates in the biosynthesis of chlorophyllide a in aerobic organisms. The same transformation is achieved in anaerobic organisms by EC 1.21.98.3, anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase. Some facultative phototrophic bacteria, such as Rubrivivax gelatinosus, possess both enzymes.
CAS REGISTRY NUMBER
COMMENTARY hide
92353-62-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
protochlorophyllide a + NADP+ + H2O
show the reaction diagram
-
intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a
-
?
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
divinylprotochlorophyllide + NADP+ + 2 H2O
show the reaction diagram
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
protochlorophyllide a + NADP+ + H2O
show the reaction diagram
-
intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
protochlorophyllide a + NADP+ + H2O
show the reaction diagram
-
intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a
-
?
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
divinylprotochlorophyllide + NADP+ + 2 H2O
show the reaction diagram
-
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
protochlorophyllide a + NADP+ + H2O
show the reaction diagram
-
intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
requirement
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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chelation of Fe2+
2,2'-bipyridyl
-
chelation of Fe2+
4-chloromercuribenzene sulfonate
-
partial inhibition of intact chloroplasts at 2.5 mM
EDTA
-
chelation of Fe3+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbate
-
prevents degradation of tetrapyrroles by reactive oxygen species
catalase
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prevents degradation of tetrapyrroles by reactive oxygen species
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isoascorbate
-
prevents degradation of tetrapyrroles by reactive oxygen species
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
green alga
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
crd1 strains fail to accumulate photosystem 1 (PS 1) and light-harvesting complex 1 (LHC 1) during hypoxia or copper deficiency, and have reduced amounts of LHC 2, Crd1 abundance is increased in copper or oxygen deficient cells
metabolism
protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway
malfunction
Cth1 accumulates in copper-sufficient, oxygenated cells
metabolism
protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CRD1_CHLRE
407
0
47212
Swiss-Prot
Mitochondrion (Reliability: 5)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster)
heterodimer
2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bollivar, D.W.; Beale, S.I.
The chlorophyll biosynthetic enzyme Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase. Characterization and partial purification from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803
Plant Physiol.
112
105-114
1996
Chlamydomonas reinhardtii, Synechocystis sp.
Manually annotated by BRENDA team
Liu, H.; Zheng, C.
MPEC: An important gene in the chlorophyll biosynthesis pathway in photosynthetic organisms
Photosynthetica
46
321-328
2008
Arabidopsis thaliana (Q9M591), Brassica napus (Q7XJI2), Chlamydomonas reinhardtii (Q9AR22), Chlamydomonas reinhardtii (Q9LD46), Cucumis sativus, Hordeum vulgare (Q5EFU4), Ipomoea nil (Q40093), Nicotiana tabacum, Oryza sativa, Rosa davurica, Rubrivivax gelatinosus (P0DJN9), Salix babylonica (Q7XJI4), Spinacia oleracea (Q7XJI5), Trifolium repens (Q7XJI1), Triticum aestivum (Q7XJI6)
-
Manually annotated by BRENDA team