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Enzyme Nomenclature
Information on EC 1.14.13.78 - ent-kaurene oxidase
Word Map on EC 1.14.13.78
- 1.14.13.78
- gibberellin
-
ent-kaurenoic
- fujikuroi
-
diterpene
-
diterpenoids
-
ent-copalyl
-
geranylgeranyl
- biotechnology
- uniconazole
-
3-oxidase
-
internode
- paclobutrazol
- cyp707a
-
8'-hydroxylase
- ga20ox
-
della
- molecular biology
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
1.14.13.78
-
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RECOMMENDED NAME
GeneOntology No.
ent-kaurene oxidase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ent-kaur-16-en-19-al + NADPH + O2 = ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
-
ent-kaur-16-en-19-ol + NADPH + H+ + O2 = ent-kaur-16-en-19-al + NADP+ + 2 H2O
-
-
-
-
ent-kaur-16-ene + NADPH + H+ + O2 = ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating)
Requires cytochrome P-450. Catalyses three sucessive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
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CAS REGISTRY NUMBER
COMMENTARY
149565-67-3
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Gibberella fujikuroi or G. intermedia, MP-C or MP-D, strains IMI58289 and D02945, gene P450-4
SwissProt
ent-kaurene oxidase 1; cv. Grand Rapids, genes LsKO1 and LsKO2 encoding ent-kaurene oxidases 1 and 2
UniProt
ent-kaurene oxidase 2; cv. Grand Rapids, genes LsKO1 and LsKO2 encoding ent-kaurene oxidases 1 and 2
UniProt
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
physiological function
metabolism
-
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
metabolism
-
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
-
physiological function
-
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
physiological function
-
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ent-kaur16-en-19-al + NADPH + H+ + O2
ent-kaur16-en-19-oate + NADP+ + H2O
-
three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
a three step oxidation
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
Q68YV8
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
Q68YV8
a three step oxidation
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
Q68YV7, Q68YV9, Q6GZ42
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
Q68YV7, Q68YV9, Q6GZ42
a three step oxidation
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
-
last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
-
a three step oxidation
-
-
?
ent-kaur-16-en-19-al + NADPH + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-en-19-al + NADPH + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-en-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + 2 H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + 2 H2O
-
-
-
?
ent-kaur-16-ene + 3 NADPH + 3 H+ + 3 O2
ent-kaur-16-en-19-oate + 3 NADP+ + 4 H2O
-
-
-
-
?
ent-kaur-16-ene + 3 NADPH + 3 H+ + 3 O2
ent-kaur-16-en-19-oate + 3 NADP+ + 4 H2O
-
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur16-en-19-ol + NADP+ + H2O
-
deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 6% yield of end-product ent-kaurenoate with ent-kaurene as substrate
-
-
?
ent-kaur-16-ene + NADPH + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-ene + NADPH + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
part of gibberelin biosynthetic pathway, decreased KO levels increase the sensitivity to KO inhibitors
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + O2
ent-kaur-16-en-19-oic acid + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + O2
ent-kaur-16-en-19-oic acid + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
-
?
ent-kaur16-en-19-ol + NADPH + H+ + O2
ent-kaur16-en-19-al + NADP+ + H2O
-
-
-
-
?
ent-kaur16-en-19-ol + NADPH + H+ + O2
ent-kaur16-en-19-al + NADP+ + H2O
-
deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 30% yield of end-product ent-kaurenoate with ent-kaurenol as substrate
-
-
?
ent-kaurene + NADPH + H+ + O2
(-)-kaurenoic acid + NADP+ + H2O
-
-
-
?
additional information
?
-
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
-
additional information
?
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
-
additional information
?
-
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
-
additional information
?
-
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
-
additional information
?
-
Q68YV8
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
-
additional information
?
-
Q68YV7
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
Q68YV9
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
Q6GZ42
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
-
additional information
?
-
-
the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ent-kaur-16-ene-19-al + NADPH + H+ + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
O94142
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + H2O
O94142
involved in gibberilin biosynthesis pathway
-
?
ent-kaur16-en-19-al + NADPH + H+ + O2
ent-kaur16-en-19-oate + NADP+ + H2O
-
three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase
-
-
?
ent-kaur16-en-19-ol + NADPH + H+ + O2
ent-kaur16-en-19-al + NADP+ + H2O
-
-
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
Q68YV8
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
Q68YV7, Q68YV9, Q6GZ42
the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
-
-
?
(-)-kaurene + 3 NADPH + 3 H+ + 3 O2
(-)-kaurenoic acid + 3 NADP+ + 4 H2O
-
last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview
-
-
?
ent-kaur-16-en-19-al + NADPH + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-en-19-al + NADPH + O2
ent-kaur-16-en-19-oate + NADP+ + H2O
Q93ZB2
-
-
-
?
ent-kaur-16-en-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + 2 H2O
-
-
-
-
?
ent-kaur-16-en-19-ol + NADPH + H+ + O2
ent-kaur-16-en-19-al + NADP+ + 2 H2O
Q93ZB2
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
Q93ZB2
-
-
-
?
ent-kaur-16-ene + NADPH + H+ + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
O94142
involved in gibberilin biosynthesis pathway
-
?
ent-kaur-16-ene + NADPH + O2
ent-kaur-16-en-19-ol + NADP+ + H2O
-
part of gibberelin biosynthetic pathway, decreased KO levels increase the sensitivity to KO inhibitors
-
-
?
ent-kaur-16-ene-19-al + NADPH + O2
ent-kaur-16-en-19-oic acid + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-ene-19-al + NADPH + O2
ent-kaur-16-en-19-oic acid + NADP+ + H2O
-
-
-
-
?
ent-kaur-16-ene-19-ol + NADPH + O2
ent-kaur-16-en-19-al + NADP+ + H2O
-
-
-
-
?
ent-kaurene + NADPH + H+ + O2
(-)-kaurenoic acid + NADP+ + H2O
F2ZAE0
-
-
-
?
additional information
?
-
-
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
-
additional information
?
-
Q93ZB2
the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
-
-
-
additional information
?
-
B5MEX5
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
-
additional information
?
-
B5MEX6
the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
-
-
-
additional information
?
-
Q68YV8
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
-
additional information
?
-
Q68YV7
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
Q68YV9
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
Q6GZ42
the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
-
-
-
additional information
?
-
-
CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
-
-
-
additional information
?
-
-
the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(1E)-1-[4-(4-butyl-1H-1,2,3-triazol-1-yl)phenyl]-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol
-
-
(1E,3S)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol
-
i.e. uniconazole
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate
-
i.e. abscinazole-E1, orUT1-E2Ts, or Abz-E1, a specific potent inhibitor of ABA 8'-hydroxylase, EC 1.14.13.93, but a weak inhibitor of CYP701A, both in vitro and in vivo
methyl (2E)-3-[1-[(1E)-1-(4-chlorophenyl)-3-hydroxy-4,4-dimethylpent-1-en-2-yl]-1H-imidazol-5-yl]prop-2-enoate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cytochrome P450 monooxygenase
-
necessary for ent-kaurenoate production by ent-kaurene oxidase
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00097
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate
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pH 7.25, 30°C, recombinant enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
2.19 Bq kaurenoids/min/mg, activity in cell extracts
additional information
Q68YV8
gibberellic acid content in healthy and rice dwarf virus-infected plants
additional information
Q68YV7
gibberellic acid content in healthy and rice dwarf virus-infected plants; gibberellic acid content in healthy and rice dwarf virus-infected plants; gibberellic acid content in healthy and rice dwarf virus-infected plants
additional information
Q68YV9
gibberellic acid content in healthy and rice dwarf virus-infected plants; gibberellic acid content in healthy and rice dwarf virus-infected plants; gibberellic acid content in healthy and rice dwarf virus-infected plants
additional information
Q6GZ42
gibberellic acid content in healthy and rice dwarf virus-infected plants; gibberellic acid content in healthy and rice dwarf virus-infected plants; gibberellic acid content in healthy and rice dwarf virus-infected plants
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
the enzyme expression in pistillate flowers is significantly higher than in staminate flowers and neutral flowers
additional information
the expression level is especially abundant in root and stem apexes
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant CYP701A3 partially in microsomal membranes from Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CYP701A, expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method
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CYP701A3, functional expression in Escherichia coli using a fully codon-optimized construct, along with additional N-terminal deletion and modification
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DNA and amino acid sequence determimnation and analysis, expression in Escherichia coli, Saccharomyces cerevisiae, and in transgenic Arabidopsis thaliana leaves as GFP-tagged protein via Agrobacterium tumefaciens transfection, expression of the GFP-tagged enzyme in onion bulb epidermis or tobacco seedling leaves using particle bombardment method
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DNA and amino acid sequence determination and analysis, sequence comparisons; KOS2, DNA and amino acid sequence determination and analysis, sequence comparisons; KOS4, DNA and amino acid sequence determination and analysis, sequence comparisons
Q68YV7, Q68YV9, Q6GZ42
gene LsKO1, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis, expression in Pichia pastoris; gene LsKO2, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis, expression in Pichia pastoris
gene P450-4 from strain D02945, DNA and amino acid sequence determination and analysis, organization of the GA gene cluster, expression in Escherichia coli and in strain D02945, screening for ectopic integrations, P450-4 promoter studies using the GUS reporter system, expression analysis
KOS3, DNA and amino acid sequence determination and analysis, sequence comparisons
Q68YV8
PCR-amplification of cDNA, cloning into pPICZ yeast expression vector, expression in Pichia pastoris strain X-33 together with fungal P450 reductase (CPR)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview
additional information
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usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
molecular biology
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the fungal cytochrome P450 monooxygenase isolated from from Phaeoshaeria sp., strain L487 in the Pichia pastoris expression system might become a tool for functional assays of a variety of rice cytochrome P450 monooxygenases involved in the biosynthesis of secondary metabolites
biotechnology
Q68YV7, Q68YV9, Q6GZ42
P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase; P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase; P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase
biotechnology
Q68YV8
P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.78)
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