Information on EC 1.14.13.74 - 7-deoxyloganin 7-hydroxylase

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The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
1.14.13.74
-
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RECOMMENDED NAME
GeneOntology No.
7-deoxyloganin 7-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
7-deoxyloganin + NADPH + H+ + O2 = loganin + NADP+ + H2O
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Metabolic pathways
-
-
Monoterpenoid biosynthesis
-
-
secologanin and strictosidine biosynthesis
-
-
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SYSTEMATIC NAME
IUBMB Comments
7-deoxyloganin,NADPH:oxygen oxidoreductase (7alpha-hydroxylating)
A heme-thiolate protein (P-450).
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CAS REGISTRY NUMBER
COMMENTARY hide
335305-40-3
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-deoxyloganic acid + NADPH + H+ + O2
loganic acid + NADP+ + H2O
show the reaction diagram
-
50% of activity with 7-deoxyloganin
-
?
7-deoxyloganin + NADPH + H+ + O2
loganin + NADP+ + H2O
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7-deoxyloganin + NADPH + H+ + O2
loganin + NADP+ + H2O
show the reaction diagram
-
enzyme is involved in loganin biosynthesis
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
45% of activity with NADPH
NADPH
-
essential for activity
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ancymidol
-
0.01 mM, 58.4% inhibition, 0.1 mM, 77.0% inhibition
CO
-
60% inhibition in the dark, partially reversible by light
cytochrome c
-
0.01 mM, 44.9% inhibition, 0.1 mM, 59.6% inhibition
ketoconazole
-
0.01 mM, 93% inhibition, 0.1 mM, complete inhibition
Metyrapone
-
0.01 mM, 73.6% inhibition, 0.1 mM, 92.4% inhibition
miconazole
-
0.01 mM, 26.2% inhibition, 0.1 mM, 78.5% inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
7-deoxyloganin
-
-
0.018
NADPH
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000099
-
activity in cell extracts, cofactor NADPH
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
50% of maximal activity at pH 7.1 and pH 9.4
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.74)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)