HOME
login
history
all enzymes
BRENDA home
History
Enzyme Nomenclature
Information on EC 1.14.13.73 - tabersonine 16-hydroxylase
Word Map on EC 1.14.13.73
- 1.14.13.73
- vindoline
- roseus
- catharanthus
-
alkaloid
- indole
-
breakpoints
- vinblastine
- epidermis
-
nonrandom
- periwinkle
- vincristine
-
madagascar
-
abrasion
-
terpenoid
- geraniol
-
searle
-
catharanthine
-
unbalanced
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Catharanthus roseus
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER 
COMMENTARY 
1.14.13.73
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
RECOMMENDED NAME
GeneOntology No.
tabersonine 16-hydroxylase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
tabersonine + NADPH + H+ + O2 = 16-hydroxytabersonine + NADP+ + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
tabersonine,NADPH:oxygen oxidoreductase (16-hydroxylating)
A heme-thiolate protein (P-450).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
250378-34-8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
50 different flowering cultivars, one low vindoline accumulating cultivar Little Delicata
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
metabolism
the enzyme catalyzes the first step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. Absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview
metabolism
CYP71D12 (isoform T16H2) plays a role in the biosynthesis of vindoline in flowers; CYP71D351 (isoform T16H2) has a major role in the biosynthesis of vindoline in leaves
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-dihydro-3-hydroxytabersonine + NADPH + H+ + O2
?
low activity
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
-
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
highest activity
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
NADH is less active as substrate
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
first step in vindoline boxynthesis
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
indole alkaloid synthesis
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
step in monoterpenoid indole alkaloid biosynthesis, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
-
-
?
additional information
?
-
-
alkaloid and enzymatic profiling of the vindoline biosynthetic pathway, overview
-
-
-
additional information
?
-
-
the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
-
-
-
additional information
?
-
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-dihydro-3-hydroxytabersonine + NADPH + H+ + O2
?
C0KYN4
low activity
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
-
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
C0KYN4
-
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
C0KYN4
highest activity
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
first step in vindoline boxynthesis
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
P98183
indole alkaloid synthesis
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
-
step in monoterpenoid indole alkaloid biosynthesis, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
-
-
?
additional information
?
-
-
alkaloid and enzymatic profiling of the vindoline biosynthetic pathway, overview
-
-
-
additional information
?
-
-
the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
-
-
-
additional information
?
-
C0KYN4
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
CO
-
concentration dependent competitive inhibition, 77% inhibition at 90%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
highest expression of isoform T16H2, CYP71D12 in young leaves
additional information
additional information
CYP71D12 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds; CYP71D351 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
T16H is anchored to the endoplasmic reticulum as a monomer via a putative 18-residue N-terminal helix
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
T16H expression analysis in leaves, overview, recombinant expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.73)
html completed
Information
