Information on EC 1.14.13.58 - benzoyl-CoA 3-monooxygenase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY
1.14.13.58
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RECOMMENDED NAME
GeneOntology No.
benzoyl-CoA 3-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
benzoyl-CoA + NADPH + H+ + O2 = 3-hydroxybenzoyl-CoA + NADP+ + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
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-
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reduction
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PATHWAY
KEGG Link
MetaCyc Link
3-phenylpropionate degradation
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SYSTEMATIC NAME
IUBMB Comments
benzoyl-CoA,NADPH:oxygen oxidoreductase (3-hydroxylating)
The enzyme from the denitrifying bacterium Pseudomonas KB740 catalyses a flavin-requiring reaction (FAD or FMN). Benzoate is not a substrate.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Benzoyl-CoA 3-hydroxylase
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Oxygenase, benzoyl coenzyme A 3-mono
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CAS REGISTRY NUMBER
COMMENTARY
151616-61-4
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain KB740
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Manually annotated by BRENDA team
Pseudomonas sp. KB740
strain KB740
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzoyl-CoA + NADPH + O2
3-hydroxybenzoyl-CoA + NADP+ + H2O
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. KB740
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no activity with NADH
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Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
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enzyme is specifically induced during aerobic growth with benzoate, second enzyme in a variant of the aerobic benzoate degradation pathway
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Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
-
second enzyme in a variant of the aerobic benzoate degradation pathway
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-
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Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
Pseudomonas sp. KB740
-
enzyme is specifically induced during aerobic growth with benzoate, second enzyme in a variant of the aerobic benzoate degradation pathway
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-
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Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
Pseudomonas sp. KB740
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second enzyme in a variant of the aerobic benzoate degradation pathway
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
-
enzyme is specifically induced during aerobic growth with benzoate, second enzyme in a variant of the aerobic benzoate degradation pathway
-
-
-
Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
-
second enzyme in a variant of the aerobic benzoate degradation pathway
-
-
-
Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
Pseudomonas sp. KB740
-
enzyme is specifically induced during aerobic growth with benzoate, second enzyme in a variant of the aerobic benzoate degradation pathway
-
-
-
Benzoyl-CoA + NADPH + O2
?
show the reaction diagram
Pseudomonas sp. KB740
-
second enzyme in a variant of the aerobic benzoate degradation pathway
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COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
FAD
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Km: 0.15 mM; required
FAD
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reaction is strictly dependent on the presence of a flavin nucleotide, FMN or FAD as cofactor
FMN
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reaction is strictly dependent on the presence of a flavin nucleotide, FMN or FAD as cofactor
NADPH
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required
NADPH
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no activity with NADH; required
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.003
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benzoyl-CoA
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0.125
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NADPH
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.025
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1.812
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
8.5
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50% of maximal activity at pH 6.5 and at pH 8.5
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Pseudomonas sp. KB740
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grown aerobically
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
65000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
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1 * 63000, SDS-PAGE
monomer
Pseudomonas sp. KB740
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1 * 63000, SDS-PAGE
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STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70°C, 50% loss of activity after 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
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