Information on EC 1.14.13.37 - methyltetrahydroprotoberberine 14-monooxygenase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.14.13.37
-
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RECOMMENDED NAME
GeneOntology No.
methyltetrahydroprotoberberine 14-monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-N-methylcanadine + NADPH + H+ + O2 = allocryptopine + NADP+ + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
chelerythrine biosynthesis
-
-
Isoquinoline alkaloid biosynthesis
-
-
Metabolic pathways
-
-
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SYSTEMATIC NAME
IUBMB Comments
(S)-N-methylcanadine,NADPH:oxygen oxidoreductase (14-hydroxylating)
A heme-thiolate protein (P-450).
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CAS REGISTRY NUMBER
COMMENTARY hide
113478-42-5
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Corydalis vaginans
papaveraceae
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-cis-N-methylcanadine + NADPH + O2
allocryptopine + NADP+ + H2O
show the reaction diagram
(S)-cis-N-methylcorydalmine + NADPH + O2
muramine + NADP+ + H2O
show the reaction diagram
Corydalis vaginans
-
-
-
-
?
(S)-cis-N-methylstylopine + NADPH + O2
protopine + NADP+ + H2O
show the reaction diagram
Corydalis vaginans
-
-
-
?
(S)-cis-N-methyltetrahydropalmitine + NADPH + O2
muramine + NADP+ + H2O
show the reaction diagram
Corydalis vaginans
-
-
-
-
?
(S)-cis-N-methyltetrahydrothalifendine + NADPH + O2
thalictrisine + NADP+ + H2O
show the reaction diagram
Corydalis vaginans
-
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-cis-N-methylcanadine + NADPH + O2
allocryptopine + NADP+ + H2O
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
Corydalis vaginans
-
-
-
NADH
Corydalis vaginans
-
33% of the activity with NADPH, NAD+ or NADP+ are ineffective
NADPH
Corydalis vaginans
-
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ancymidol
Corydalis vaginans
-
-
CO
Corydalis vaginans
-
reversible by light
cytochrome c
Corydalis vaginans
-
-
ketoconazole
Corydalis vaginans
-
-
Metyrapone
Corydalis vaginans
-
-
Prochloraz
Corydalis vaginans
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0125
cis-N-methylcanadine
Corydalis vaginans
-
-
0.0625
NADPH
Corydalis vaginans
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0255
Corydalis vaginans
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
Corydalis vaginans
-
-
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.7
Corydalis vaginans
-
half-maximal activity at pH 6.5 and pH 9.7
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Corydalis vaginans
-
-
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 45
Corydalis vaginans
-
half-maximal activity at 10°C and 45°C
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Corydalis vaginans
-
-
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Corydalis vaginans
-
membrane bound
-
Manually annotated by BRENDA team
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.37)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)