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Enzyme Nomenclature
Information on EC 1.14.13.30 - leukotriene-B4 20-monooxygenase
Word Map on EC 1.14.13.30
- 1.14.13.30
- warfarin
- cyp2c9
- vkorc1
-
arachidonic
- cyp4fs
-
omega-hydroxylation
-
anticoagulant
-
dosing
- 20-hete
-
pharmacogenetic
- cyp4a11
-
20-hydroxyeicosatetraenoic
-
eicosanoids
- cyp3a5
-
omega-oxidation
-
acenocoumarol
- cyp1b1
-
thromboembolic
-
epoxyeicosatrienoic
-
lipoxins
-
ebastine
-
tocotrienols
-
20-hydroxy-ltb4
-
17-octadecynoic
-
haplotype-based
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
1.14.13.30
-
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RECOMMENDED NAME
GeneOntology No.
leukotriene-B4 20-monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + H+ + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
a heme-thiolate protein P450; not identical with EC 1.14.13.34
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
omega-oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate,NADPH:oxygen oxidoreductase (20-hydroxylating)
A heme-thiolate protein (P-450). Not identical with EC 1.14.13.34, leukotriene-E4 20-monooxygenase.
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CAS REGISTRY NUMBER
COMMENTARY
90119-11-2
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
CYP4F catalyzes the omega-hydroxylation of the side chain of tocopherols and tocotrienols, the first step in their catabolism to polar metabolites excreted in urine. CYP4F2 is the only enzyme currently shown to metabolize vitamin E in humans
physiological function
physiological function
-
by regulating the balance of leukotriene B4 in the lung, LTB4OH may function as a suppressor of lung carcinogenesis
physiological function
-
is critical in the inactivation of leukotriene B4 in polymorphonuclear leukocytes with a -fold induction between 3 and 71
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
(E,Z,Z,Z)-8-hydroxy-5,9,11,14-eicosatetraenoic acid + NADPH + O2
?
-
CYP4F2
-
-
?
(Z9(10))-epoxyoctadecanoic acid + NADPH + O2
?
-
omega-hydroxylation activity and weak (omega-1)-hydroxylation activity
-
-
?
6-trans-12-epi-leukotriene B4 + NADPH + O2
6-trans-12-epi-20-hydroxy-leukotriene B4
-
-
-
-
?
alpha-tocopherol + NADPH + H+ + O2
alpha-13'-hydroxy-tocopherol + NADP+ + H2O
-
-
-
-
?
delta-tocopherol + NADPH + H+ + O2
delta-13'-hydroxy-tocopherol + NADP+ + H2O
-
-
-
-
?
gamma-tocopherol + NADPH + H+ + O2
gamma-13'-hydroxy-tocopherol + NADP+ + H2O
-
-
-
-
?
leukotriene B4 + NADPH + H+ + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
leukotriene B4 + NADPH + O2
?
-
induction of in all-trans-retinoic acid-treated HL60 cells
-
-
?
prostaglandin H1 + NADPH + O2
19-hydroxy-prostaglandin H1 + NADP+ + H2O
-
CYP4F8
-
-
?
prostaglandin H2 + NADPH + O2
19-hydroxy-prostaglandin H2 + NADP+ + H2O
-
CYP4F8
-
-
?
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
inactivation of leukotriene B4, a mediator of inflammation
-
-
-
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
-
-
-
?
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
LTB4 inactivation, in vivo inhibition of CYP4F18 results in a marked increase in calcium flux and a 220% increases in the chemotactic response of mouse polymorphonuclear leukocytes to LTB4
-
-
?
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
inactivation of leukotriene B4, a mediator of inflammation
-
-
-
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
inactivation of leukotriene B4, a mediator of inflammation
-
-
-
arachidonate + NADPH + O2
20-hydroxy-5,8,11,14-eicosatetraenoic acid + NADP+ + H2O
-
-
-
-
?
arachidonate + NADPH + O2
20-hydroxy-5,8,11,14-eicosatetraenoic acid + NADP+ + H2O
-
very poor substrate
-
-
?
arachidonate + NADPH + O2
20-hydroxy-5,8,11,14-eicosatetraenoic acid + NADP+ + H2O
-
the enzyme is the principal arachidonate omega-hydroxylase in liver, CYP4F2
-
-
?
leukotriene B4 + NADPH + H+ + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
-
-
?
leukotriene B4 + NADPH + H+ + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
CYP4F3A is expressed in human leukocytes and catalyzes the omega-hydroxylation of LTB4
-
-
?
leukotriene B4 + NADPH + H+ + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
CYP4F3A catalyzes the omega-hydroxylation of LTB4
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
-
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
the enzyme plays a critical role in regulating the biological effect of leukotriene B4, CYP4F3
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
yielding omega-COOH-leukotriene B4 via omega-hydroxy-leukotriene B4 and omega-CHO-leukotriene B4, CYP4F3
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
all recombinant proteins generate product I from leukotriene B4, which in all cases is indistinguishable from the 20-hydroxy-leukotriene B4 reference compound assayed under similar conditions. CYP4F3A is the most efficient leukotriene B4 20-hydroxylase. CYP4F3A and 4F3B generate a secondary product, which is 20-COOH leukotriene B4 derived from 20-hydroxy-leukotriene B4. CYP4F12 is least efficient, generating several additional products. Product I derived from CYP4F3A and that from epidermal keratinocytes are identical to the 20-hydroxy-leukotriene B4 reference compound
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
-
-
?
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
-
-
?
lipoxin A4 + NADPH + O2
?
-
CYP4F3 preferentially omega-jydroxylates lipoxin B4 over lipoxin A4
-
-
?
lipoxin B4 + NADPH + O2
?
-
CYP4F3 preferentially omega-jydroxylates lipoxin B4 over lipoxin A4
-
-
?
prostaglandin A1 + NADPH + O2
20-hydroxy-prostaglandin A1 + NADP+ + H2O
recombinant CYP4F14
-
-
?
prostaglandin A1 + NADPH + O2
20-hydroxy-prostaglandin A1 + NADP+ + H2O
-
-
-
-
?
additional information
?
-
-
overview, stereochemical requirement for substrate specificity
-
-
-
additional information
?
-
-
recombinant protein CYP4F3, wide substrate specificity
-
-
-
additional information
?
-
-
CYP4F3B and CYP4F2 may function collaboratively as 20-HETE synthetase as well as omega-hydroxylases for leukotiene b4 and other lipoxygenase-dependent eicosanoids in human liver and other tissues
-
-
-
additional information
?
-
-
no activity with 20-hydroxy-leukotriene B4 and lipoxin B4
-
-
-
additional information
?
-
cytochrome P450 4F2 gene encodes for the major CYP enzyme responsible for the synthesis of 20-hydroxyeicosatetraenoic acid in the human kidney
-
-
-
additional information
?
-
-
leukotriene B4 and arachidonate omega-hydroxylase activities are both CYP4F2-catalyzed in the liver, overview
-
-
-
additional information
?
-
-
CYP4F3A also catalyzes the omega-hydroxylation of epoxyeicosatrieonic acids
-
-
-
additional information
?
-
-
CYP4F18 is a critical protein in the regulation of LTB4 metabolism, the enzyme is active in down-regulation of responses to leukotriene B4, i.e. LTB4, leukotriene B4 is a potent chemoattractant for polymorphonuclear leukocytes and other cells, overview
-
-
-
additional information
?
-
-
lipopolysaccharide treatament suppresses CYP4F4 and upregulates CYP4F5 mRNA expression in rat liver whereas renal CYP4Fs are essentially unchanged. BaSO4 treatment, in contrast, increases both hepatic and renal CYP4F expression levels
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
arachidonate + NADPH + O2
20-hydroxy-5,8,11,14-eicosatetraenoic acid + NADP+ + H2O
-
the enzyme is the principal arachidonate omega-hydroxylase in liver, CYP4F2
-
-
?
leukotriene B4 + NADPH + H+ + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
leukotriene B4 + NADPH + O2
20-hydroxy-leukotriene B4 + NADP+ + H2O
-
the enzyme plays a critical role in regulating the biological effect of leukotriene B4, CYP4F3
-
-
?
leukotriene B4 + NADPH + O2
?
-
induction of in all-trans-retinoic acid-treated HL60 cells
-
-
?
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
inactivation of leukotriene B4, a mediator of inflammation
-
-
-
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
LTB4 inactivation, in vivo inhibition of CYP4F18 results in a marked increase in calcium flux and a 220% increases in the chemotactic response of mouse polymorphonuclear leukocytes to LTB4
-
-
?
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
inactivation of leukotriene B4, a mediator of inflammation
-
-
-
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
inactivation of leukotriene B4, a mediator of inflammation
-
-
-
leukotriene B4 + NADPH + H+ + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
P78329
-
-
-
?
leukotriene B4 + NADPH + H+ + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP+ + H2O
-
CYP4F3A is expressed in human leukocytes and catalyzes the omega-hydroxylation of LTB4
-
-
?
additional information
?
-
-
CYP4F3B and CYP4F2 may function collaboratively as 20-HETE synthetase as well as omega-hydroxylases for leukotiene b4 and other lipoxygenase-dependent eicosanoids in human liver and other tissues
-
-
-
additional information
?
-
P78329
cytochrome P450 4F2 gene encodes for the major CYP enzyme responsible for the synthesis of 20-hydroxyeicosatetraenoic acid in the human kidney
-
-
-
additional information
?
-
-
leukotriene B4 and arachidonate omega-hydroxylase activities are both CYP4F2-catalyzed in the liver, overview
-
-
-
additional information
?
-
-
CYP4F18 is a critical protein in the regulation of LTB4 metabolism, the enzyme is active in down-regulation of responses to leukotriene B4, i.e. LTB4, leukotriene B4 is a potent chemoattractant for polymorphonuclear leukocytes and other cells, overview
-
-
-
additional information
?
-
-
lipopolysaccharide treatament suppresses CYP4F4 and upregulates CYP4F5 mRNA expression in rat liver whereas renal CYP4Fs are essentially unchanged. BaSO4 treatment, in contrast, increases both hepatic and renal CYP4F expression levels
-
-
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
all-trans-retinoic acid
-
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f13, 4f14, and 4f37 are down-regulated. No changes in transcript abundance for the Cyp4f16, 4f17, and 4f18 genes
additional information
-
in vivo inhibition of CYP4F18 results in a marked increase in calcium flux and a 220% increase in the chemotactic response of mouse PMN to LTB4
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
all-trans retinoic acid
-
significantly induces expression of CYP4F3A at both mRNA and protein levels in cultured HL-60 cells
all-trans-retinoic acid
-
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f39 are up-regulated. No changes in transcript abundance for the Cyp4f16, 4f17, and 4f18 genes
lipopolysaccharide
-
upregulates expression and activity of LTB4OH in ex-smokers in a dose-dependent manner, but not in smokers. In vitro smoke exposure of ex-smokers' bronchoalveolar lavage cells abrogates the lipopolysaccharide-induced up-regulation of LTB4OH mRNA expression, thus tobacco smoke alters bronchoalveolar lavage cell LTB4OH mRNA expression in response to lipopolysaccharide exposure
phenol
-
significantly induces expression of CYP4F3A at both mRNA and protein levels in cultured and differentiated HL-60 cells, in the proerythroid cell line K-562, and ex vivo in human neutrophils
additional information
-
CYP4F18 expression is induced in bone marrow-derived dendritic cells by bacterial lipopolysaccharide, a ligand for TLR4, and by poly(I-C), a ligand for TLR3
-
additional information
-
hepatic enzyme is not induced by phenobarbital or 3-methylcholanthrene
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8 - 9.5
-
pH 6.8: about 50% of activity maximum, pH 9.5: about 70% of activity maximum
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
ex-smokers express significantly higher LTB4OH mRNA levels than smokers
-
exhibits greater leukotriene B4 hydroxylase activities than fresh skin tissues of either species
-
CYP4F3 is specifically expressed in. CYP4F3 is restricted to myeloid cells
additional information
-
CYP4F11; CYP4F2; CYP4F3B is the predominant CYP4F isoform; mRNA for CYP4F8 detected
-
transcript abundance is highest for Cyp4f13 and 4f16 genes, lowest for Cyp4f14, 4f17, and 4f37, and intermediate for Cyp4f18 and 4f39. Transcripts arising from Cyp4f15 are highly variable, ranging from intermediate to undetectable levels in individual mice. Transcripts arising from Cyp4f40 are present, but levels are too low to measure reliably in most mice
-
transcript abundance is highest for Cyp4f13 and 4f16 genes, lowest for Cyp4f14, 4f17, and 4f37, and intermediate for Cyp4f18 and 4f39. Transcripts arising from Cyp4f15 are highly variable, ranging from intermediate to undetectable levels in individual mice. Transcripts arising from Cyp4f40 are present, but levels are too low to measure reliably in most mice
-
additional information
-
CYP4F2 is not expressed in myeloid cells, polymorphonuclear leukocyte; no expression of CYP4F8 in leukocytes
additional information
-
tissue-specific expression and function of CYP4F2, overview
additional information
-
tissue-specific expression, expression analysis of CYP4F18 in different cell types, tissue distribution, overview
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
61000
-
recombinant isoforms CYP4F3A and CYP4F3B expressed in COS-7 cells, western blot analysis
additional information
-
the isolated cDNA encodes a protein of 520 amino acids with 59805 Da, CYP4F3
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, no loss of activity with highly purified preparations even after 4 years
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from insect Sf9 suspension cultures, to near homogeneity, native enzyme partially from liver and kidney by microsome preparation
-
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
chromosomal localization 19p; CYP4F3 gene contains 14 exons and 13 introns; expression in transfected COS-7 cells; tissue-specific expression is regulated by alternative promoter usage and mutually exclusive exon splicing, exon 3 and 4; two isoforms of CYP4F3, CYP4F3A expressed in neutrophils and CYP4F3B expressed in fetal and adult liver
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expression in Spodoptera frugiperda Sf9 cells using the baculovirus infection system
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expression of wild-type and CYP4F2 mutant enzymes in Spodoptera frugiperda SF9 insect cell microsomes via the baculovrius transfection system
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gene Cyp4f18, expression analysis of CYP4F18 in different cell types, recombinant expression of the enzyme in Spodoptera frugiperda Sf9 cell microsomes via baculovirus transfection system
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wild-type and mutant DNA and amino acid sequence determinations, functional nonsynonymous polymorphisms, overview, expression of wild-type and mutant genes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f13, 4f14, and 4f37 are down-regulated
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f39 are up-regulated
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f13, 4f14, and 4f37 are down-regulated
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after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f13, 4f14, and 4f37 are down-regulated
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after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f39 are up-regulated
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after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f39 are up-regulated
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
V433M
W12G
W12G/V433M
the functional polymorphism in human Cyp4F2 decreases 20-hydroxyeicosatetraenoic acid production, but does not influence the omega-hydroxylation of leukotriene B4, screening and genotyping of African and European Americans, overview
V433M
the functional polymorphism in human Cyp4F2 decreases 20-hydroxyeicosatetraenoic acid production, but does not influence the omega-hydroxylation of leukotriene B4, screening and genotyping of African and European Americans, overview
V433M
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naturally occuring common genetic variant of the human CYP4F2 gene, the mutant has lower enzyme specific activity toward tocopherols, 42-66% of wild-type activity, but is without a significant effect on the metabolism of tocotrienols
W12G
the mutant enzyme activity is similar to the wild-type enzyme, screening and genotyping of African and European Americans, overview
W12G
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naturally occuring common genetic variant of the human CYP4F2 gene, the mutant exhibits a greater enzyme specific activity compared to the wild-type enzyme for both tocopherols and tocotrienols, 230-275% of wild-type activity towards alpha, gamma, and delta-tocopherol and 350% of wild-type activity towards alpha, gamma, and delta-tocotrienol
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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induction of CYP4F3 may play a role in the development of benzene hematotoxicity and serve as a biomarker of benzene exposure
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.30)
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