Information on EC 1.14.13.230 - butane monooxygenase (soluble)

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The expected taxonomic range for this enzyme is: Thauera butanivorans

EC NUMBER
COMMENTARY hide
1.14.13.230
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RECOMMENDED NAME
GeneOntology No.
butane monooxygenase (soluble)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
butane + NADH + H+ + O2 = butan-1-ol + NAD+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Butanoate metabolism
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Microbial metabolism in diverse environments
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butane degradation
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SYSTEMATIC NAME
IUBMB Comments
butane,NADH:oxygen oxidoreductase
The enzyme, characterized from the bacterium Thauera butanivorans, is similar to EC 1.14.13.25, methane monooxygenase (soluble), but has a very low activity with methane. It comprises three components - a carboxylate-bridged non-heme di-iron center-containing hydroxylase (made of three different subunits), a flavo-iron sulfur-containing NADH-oxidoreductase, and a small regulatory component protein. The enzyme can also act on other C3-C6 linear and branched aliphatic alkanes with lower activity.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
show the reaction diagram
butane + NADH + H+ + O2
butan-2-ol + NAD+ + H2O
show the reaction diagram
ethane + NADH + H+ + O2
ethanol + NAD+ + H2O
show the reaction diagram
hexane + NADH + H+ + O2
hexan-1-ol + NAD+ + H2O
show the reaction diagram
isobutane + NADH + H+ + O2
2-methyl-1-propanol + NAD+ + H2O
show the reaction diagram
isopentane + NADH + H+ + O2
3-methyl-1-butanol + 2-methyl-1-butanol + NAD+ + H2O
show the reaction diagram
methane + NADH + H+ + O2
methanol + NAD+ + H2O
show the reaction diagram
pentane + NADH + H+ + O2
pentan-1-ol + NAD+ + H2O
show the reaction diagram
pentane + NADH + H+ + O2
pentan-2-ol + NAD+ + H2O
show the reaction diagram
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?
pentane + NADH + H+ + O2
pentan-3-ol + NAD+ + H2O
show the reaction diagram
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?
propan + NADH + H+ + O2
propan-1-ol + NAD+ + H2O
show the reaction diagram
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?
propane + NADH + H+ + O2
propan-1-ol + NAD+ + H2O
show the reaction diagram
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about 82% propan-1-ol plus 18% propan-2-ol, in presence and absence of regulatory subunit BmoB
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?
propane + NADH + H+ + O2
propan-2-ol + NAD+ + H2O
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
butane + NADH + H+ + O2
butan-1-ol + NAD+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Butanoate
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incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity
propanoate
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10 mM, 41% residual activity. Incubation of alkane-grown cells with butanoate or propanoate leads to irreversible time- and O2-dependent loss of butane monooxygenase activity. Propanoate-dependent inactivation involves interaction with the catalytic site. Butane monooxygenase is protected from propanoate-dependent inactivation by the presence of its natural substrate, butane
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00024
butane
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pH 7.2, 25C
0.0022
ethane
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pH 7.2, 25C
1.1
methane
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pH 7.2, 25C
0.00034
pentane
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pH 7.2, 25C
0.00094
propane
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pH 7.2, 25C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 0.48
butane
0.76
ethane
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pH 7.2, 25C
1.3
methane
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pH 7.2, 25C
0.008 - 0.34
pentane
0.55 - 0.98
propane
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2500
butane
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overall reaction, pH 7.2, 25C
350
ethane
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pH 7.2, 25C
1.2
methane
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pH 7.2, 25C
1200
pentane
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overall reaction, pH 7.2, 25C
690
propane
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overall reaction, pH 7.2, 25C
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activity in a reporter strain is not induced by butane, but is induced by 1-butanol and butyraldehyde
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L287F
Q8KQF0 and Q8KQE9 and Q8KQE7
mutation in subunit BmoX, loss of activity
P179K
Q8KQF0 and Q8KQE9 and Q8KQE7
mutation in subunit BmoX, loss of activity
Q319G
Q8KQF0 and Q8KQE9 and Q8KQE7
mutation in subunit BmoX, loss of activity
V181M
Q8KQF0 and Q8KQE9 and Q8KQE7
mutation in subunit BmoX, loss of activity
F321Y
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mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 61% residual activity
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G113N
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mutation in the alpha-subunit of hydrolase component, loss of inhibition by propanoate
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L279F
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mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 56% residual activity
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Q320K
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mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 28% residual activity
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T148C
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mutation in the alpha-subunit of hydrolase component, in presence of 10 mM propanoate, 15% residual activity
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