Information on EC 1.14.13.212 - 1,3,7-trimethyluric acid 5-monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.212
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RECOMMENDED NAME
GeneOntology No.
1,3,7-trimethyluric acid 5-monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,3,7-trimethylurate + NADH + H+ + O2 = 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
caffeine degradation V (bacteria, via trimethylurate)
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SYSTEMATIC NAME
IUBMB Comments
1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate forming)
The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3,7-trimethylurate + NADH + H+ + O2
1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O
show the reaction diagram
1,3-dimethylurate + NADH + H+ + O2
1,3-dimethyl-5-hydroxyisourate + NAD+ + H2O
show the reaction diagram
1-methylurate + NADH + H+ + O2
1-methyl-5-hydroxyisourate + NAD+ + H2O
show the reaction diagram
3,7-dimethylurate + NADH + H+ + O2
3,7-dimethyl-5-hydroxyisourate + NAD+ + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,3,7-trimethylurate + NADH + H+ + O2
1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
flavoprotein
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0102
1,3,7-trimethylurate
pH 7.5, 30C
0.1265
1,3-dimethylurate
pH 7.5, 30C
0.0012
1-Methylurate
pH 7.5, 30C
0.0013
3,7-dimethylurate
pH 7.5, 30C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.48
1,3,7-trimethylurate
pH 7.5, 30C
3.08
1,3-dimethylurate
pH 7.5, 30C
0.485
1-Methylurate
pH 7.5, 30C
1.97
3,7-dimethylurate
pH 7.5, 30C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
735
1,3,7-trimethylurate
pH 7.5, 30C
25
1,3-dimethylurate
pH 7.5, 30C
408.3
1-Methylurate
pH 7.5, 30C
1490
3,7-dimethylurate
pH 7.5, 30C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.128
purified native enzyme, pH 7.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
x * 43000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 55fold from strain CBB1 by anion exchange, affinity, and hydrophobic interaction chromatography, followed by gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene tmuM, DNA and amino acid sequence determination and analysis, genes involved in the caffeine C-8 oxidation pathway are located in a 25.2-kb genomic DNA fragment of CBB1, including cdhABC (coding for caffeine dehydrogenase) and tmuM (coding for TmuM)
gene tmuM, genes involved in the caffeine C-8 oxidation pathway are located in a 25.2-kb genomic DNA fragment of CBB1, including cdhABC (coding for caffeine dehydrogenase) and tmuM (coding for TmuM)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture