Information on EC 1.14.13.175 - aflatoxin B synthase

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The expected taxonomic range for this enzyme is: Aspergillus

EC NUMBER
COMMENTARY
1.14.13.175
-
RECOMMENDED NAME
GeneOntology No.
aflatoxin B synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2 = aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
-
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2 = aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
(1)
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
aflatoxins B1 and G1 biosynthesis
-
-
aflatoxins B2 and G2 biosynthesis
-
-
Aflatoxin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
8-O-methylsterigmatocystin,NADPH:O2 oxidoreductase (aflatoxin-B forming)
A heme-thiolate (P-450) enzyme. Isolated from the mold Aspergillus parasiticus.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aflQ
-
gene name
aflQ
Aspergillus flavus PFCC 101, Aspergillus flavus PFCC 116, Aspergillus flavus PFCC 137, Aspergillus flavus PFCC 176, Aspergillus flavus PFCC 209, Aspergillus flavus PFCC 247, Aspergillus flavus PFCC 267, Aspergillus flavus PFCC 309
-
gene name
-
aflQ
-
gene name
aflQ
Aspergillus parasiticus NRRL 2999, Aspergillus parasiticus PFCC 14
-
gene name
-
ordA
-
-
gene name
-
ordA
-
gene name
ordA
Aspergillus flavus PFCC 101, Aspergillus flavus PFCC 116, Aspergillus flavus PFCC 137, Aspergillus flavus PFCC 176, Aspergillus flavus PFCC 209, Aspergillus flavus PFCC 247, Aspergillus flavus PFCC 267, Aspergillus flavus PFCC 309
-
gene name
-
ordA
-
gene name
ordA
Aspergillus parasiticus NRRL 2999, Aspergillus parasiticus PFCC 14
-
gene name
-
ordA
Aspergillus parasiticus SRRC 143
gene name
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Aspergillus flavus PFCC 101
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus PFCC 116
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus PFCC 137
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus PFCC 176
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus PFCC 209
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus PFCC 247
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus PFCC 267
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus PFCC 309
-
-
-
Manually annotated by BRENDA team
Aspergillus flavus SRRC 141
-
-
-
Manually annotated by BRENDA team
Aspergillus parasiticus NRRL 2999
-
-
-
Manually annotated by BRENDA team
Aspergillus parasiticus PFCC 14
-
-
-
Manually annotated by BRENDA team
Aspergillus parasiticus SRRC 143
-
UniProt
Manually annotated by BRENDA team
Aspergillus parasiticus SRRC 163
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
the enzyme is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2
metabolism
Aspergillus parasiticus SRRC 143
-
the enzyme is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus parasiticus SRRC 163
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus parasiticus SRRC 143
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus flavus SRRC 141
-
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus flavus, Aspergillus parasiticus, Aspergillus parasiticus SRRC 163
-
preferred substrate
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus parasiticus SRRC 143
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus flavus SRRC 141
-
preferred substrate
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
O13345
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus parasiticus SRRC 163
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus parasiticus SRRC 143
O13345
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methyldihydrosterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B2 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus flavus SRRC 141
-
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
O13345
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus flavus, Aspergillus parasiticus, Aspergillus parasiticus SRRC 163
-
preferred substrate
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus parasiticus SRRC 143
O13345
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
-
-
-
?
8-O-methylsterigmatocystin + 2 NADPH + 2 H+ + 2 O2
aflatoxin B1 + 2 NADP+ + H2O + methanol + CO2
show the reaction diagram
Aspergillus flavus SRRC 141
-
preferred substrate
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0134
8-O-methyldihydrosterigmatocystin
-
in 50 mM phosphate buffer, pH 7.5, at 29C
0.0012
8-O-methylsterigmatocystin
-
in 50 mM phosphate buffer, pH 7.5, at 29C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.23
-
enzyme from supernatant, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29C
34.11
-
after 148.3fold purification, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Aspergillus parasiticus SRRC 143
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 60000, SDS-PAGE
?
-
x * 60000, SDS-PAGE
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain InvSc1
expressed in Saccharomyces cerevisiae strain INVSc2
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A143S
the mutation affects enzymatic activity (approximately 50% of the enzyme activity which converts O-methylsterigmatocystin to aflatoxin B1 is lost)
H400L
the mutation results in a loss of the monooxygenase activity
I528Y
the mutation does not affect enzymatic activity
A143S
Aspergillus parasiticus SRRC 143
-
the mutation affects enzymatic activity (approximately 50% of the enzyme activity which converts O-methylsterigmatocystin to aflatoxin B1 is lost)
-
H400L
Aspergillus parasiticus SRRC 143
-
the mutation results in a loss of the monooxygenase activity
-
I528Y
Aspergillus parasiticus SRRC 143
-
the mutation does not affect enzymatic activity
-