Information on EC 1.14.13.170 - pentalenolactone D synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY
1.14.13.170
-
RECOMMENDED NAME
GeneOntology No.
pentalenolactone D synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
1-deoxy-11-oxopentalenate + NADPH + H+ + O2 = pentalenolactone D + NADP+ + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
Baeyer-Villiger oxidation
oxidation
Streptomyces arenae TU46, Streptomyces arenae TUE469, Streptomyces exfoliatus UC5319
-
Baeyer-Villiger oxidation
-
PATHWAY
KEGG Link
MetaCyc Link
pentalenolactone biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (pentalenolactone-D forming)
A FAD-dependent oxygenase. Isolated from the bacteria Streptomyces exfoliatus and Streptomyces arenae. The ketone undergoes a biological Baeyer-Villiger reaction. Part of the pathway of pentalenolactone biosynthesis.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Baeyer-Villiger monooxygenase
-
-
Baeyer-Villiger monooxygenase
Streptomyces arenae TU46, Streptomyces arenae TUE469
-
-
-
Baeyer-Villiger monooxygenase
-
-
Baeyer-Villiger monooxygenase
Streptomyces exfoliatus UC5319
-
-
-
penE
-
-
gene name
-
penE
Streptomyces exfoliatus UC5319
-
-
-
pntE
-
-
gene name
-
pntE
Streptomyces arenae TU46, Streptomyces arenae TUE469
-
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Streptomyces arenae TU46
-
-
-
Manually annotated by BRENDA team
Streptomyces exfoliatus UC5319
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
pentalenolactone D + NADP+ + H2O
show the reaction diagram
Streptomyces exfoliatus, Streptomyces arenae, Streptomyces arenae TU46, Streptomyces exfoliatus UC5319, Streptomyces arenae TUE469
-
-
-
-
ir
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
FAD
-
the enzyme contains one bound FAD molecule
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry
?
-
x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry
?
Streptomyces arenae TU46, Streptomyces arenae TUE469
-
x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry
-
?
Streptomyces exfoliatus UC5319
-
x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-