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Information on EC 1.14.13.149 - phenylacetyl-CoA 1,2-epoxidase

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IUBMB Comments
Part of the aerobic pathway of phenylacetate catabolism in Escherichia coli and Pseudomonas putida.
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UNIPROT: P76079
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
paaabcde, paaac, paaabce, phenylacetyl-coa monooxygenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PaaAC
part of the phenylacetic acid-CoA,oxygenase complex
SYSTEMATIC NAME
IUBMB Comments
phenylacetyl-CoA:oxygen oxidoreductase (1,2-epoxidizing)
Part of the aerobic pathway of phenylacetate catabolism in Escherichia coli and Pseudomonas putida.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
the protein contains a di-iron center
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PAAC_ECOLI
Escherichia coli (strain K12)
248
0
27877
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotetramer
dimer of heterodimers
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, PaaAC with acetyl-CoA is crystallized in 0.1 M sodium citrate buffer, pH 5.5, and 15% (w/v) PEG 6000 (Fluka). Crystals of ligand-free PaaAC are obtained in 100 mM N-(2-acetamido)-iminodiacetic acid, pH 5.5
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superose 12 gel filtration
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Grishin, A.; Ajamian, E.; Tao, L.; Zhang, L.; Menard, R.; Cygler, M.
Structural and functional studies of the Escherichia coli phenylacetyl-CoA monooxygenase complex
J. Biol. Chem.
286
10735-10743
2011
Escherichia coli (P76079), Escherichia coli
Manually annotated by BRENDA team