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Information on EC 1.14.13.146 - taxoid 14beta-hydroxylase and Organism(s) Taxus cuspidata and UniProt Accession Q84KI1

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IUBMB Comments
Requires cytochrome P450. From the yew Taxus cuspidata. Also acts on taxa-4(20),11-dien-5alpha-yl acetate.
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Taxus cuspidata
UNIPROT: Q84KI1
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The taxonomic range for the selected organisms is: Taxus cuspidata
The enzyme appears in selected viruses and cellular organisms
Synonyms
cytochrome P450 taxoid 14beta-hydroxylase, taxoid 14beta-hydroxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cytochrome P450 taxoid 14beta-hydroxylase
-
taxoid 14beta-hydroxylase
-
SYSTEMATIC NAME
IUBMB Comments
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl-acetate,NADPH:oxygen 14-oxidoreductase
Requires cytochrome P450. From the yew Taxus cuspidata. Also acts on taxa-4(20),11-dien-5alpha-yl acetate.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5alpha-acetoxy-10beta-hydroxytaxadiene + O2 + NADPH + H+
5alpha-acetoxy-10beta,14beta-dihydroxytaxadiene + NADP+ + H2O
show the reaction diagram
-
-
-
?
5alpha-acetoxytaxadiene + O2 + NADPH + H+
? + NADP+ + H2O
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
dependent on
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.055
5alpha-acetoxy-10beta-hydroxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
0.033
5alpha-acetoxytaxadiene
in Tris-HCl pH 7.5, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
T14H_TAXCU
509
2
57147
Swiss-Prot
Secretory Pathway (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57146
x * 57146, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 57146, calculated from amino acid sequence
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae WAT11 cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
manipulation of the hydroxylase gene can permit redirection of the pathway to increase flux toward taxol and can allow the preparation of 13alpha,14beta-hydroxy taxoids as new therapeutic agents
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jennewein, S.; Rithner, C.D.; Williams, R.M.; Croteau, R.
Taxoid metabolism: Taxoid 14beta-hydroxylase is a cytochrome P450-dependent monooxygenase
Arch. Biochem. Biophys.
413
262-270
2003
Taxus cuspidata (Q84KI1)
Manually annotated by BRENDA team