Information on EC 1.14.13.144 - 9beta-pimara-7,15-diene oxidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)
Please login to have access to the AMENDA and FRENDA data


The expected taxonomic range for this enzyme is: Oryza sativa

top print hide
EC NUMBER
COMMENTARY hide
1.14.13.144
-
top print hide
RECOMMENDED NAME
GeneOntology No.
9beta-pimara-7,15-diene oxidase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9beta-pimara-7,15-dien-19-al + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-oate + NADP+ + H2O
show the reaction diagram
(1c)
-
-
-
9beta-pimara-7,15-dien-19-ol + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-al + NADP+ + 2 H2O
show the reaction diagram
(1b)
-
-
-
9beta-pimara-7,15-diene + 3 O2 + 3 NADPH + 3 H+ = 9beta-pimara-7,15-dien-19-oate + 3 NADP+ + 4 H2O
show the reaction diagram
overall reaction
-
-
-
9beta-pimara-7,15-diene + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-ol + NADP+ + H2O
show the reaction diagram
(1a)
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
diterpene phytoalexins precursors biosynthesis
-
-
Diterpenoid biosynthesis
-
-
momilactone A biosynthesis
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase
Requires cytochrome P-450. A rice, Oryza sativa, enzyme involved in the phytoalexin momilactone biosynthesis. It also acts similarly on 9beta-stemod-13(17)-ene.
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
the double knockdown of CYP99A2 and CYP99A3 specifically suppresses the elicitor-inducible production of momilactones
physiological function
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9beta-pimara-7,15-diene + O2 + NADPH + H+
3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
syn-pimara-7,15-diene + O2 + NADPH + H+
syn-pimaradien-19-ol + NADP+ + H2O
show the reaction diagram
-
-
-
?
syn-pimaradien-19-al + O2 + NADPH + H+
syn-pimaradien-19-oate + NADP+ + H2O
show the reaction diagram
-
-
-
?
syn-pimaradien-19-ol + O2 + NADPH + H+
syn-pimaradien-19-al + NADP+ + H2O
show the reaction diagram
-
-
-
?
syn-stemod-13(17)-ene + O2 + NADPH + H+
syn-stemoden-19-ol + NADP+ + H2O
show the reaction diagram
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
-
-
?
syn-stemoden-19-al + O2 + NADPH + H+
syn-stemoden-19-oate + NADP+ + H2O
show the reaction diagram
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
-
-
?
syn-stemoden-19-ol + O2 + NADPH + H+
syn-stemoden-19-al + NADP+ + H2O
show the reaction diagram
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
-
-
?
additional information
?
-
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
syn-pimara-7,15-diene
pH and temperature not specified in the publication
0.009
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
46
syn-pimara-7,15-diene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
49
syn-stemod-13(17)-ene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
top print hide
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23000
syn-pimara-7,15-diene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
41912
5000
syn-stemod-13(17)-ene
Oryza sativa
Q0JF01
pH and temperature not specified in the publication
41913
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 cells
expressed in Escherichia coli Rosetta (DE3) cells
-
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.144)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)