Information on EC 1.14.13.143 - ent-isokaurene C2-hydroxylase

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The expected taxonomic range for this enzyme is: Oryza sativa

top print hide
EC NUMBER
COMMENTARY hide
1.14.13.143
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RECOMMENDED NAME
GeneOntology No.
ent-isokaurene C2-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-isokaurene + O2 + NADPH + H+ = ent-2alpha-hydroxyisokaurene + H2O + NADP+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxylation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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oryzalide A biosynthesis
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diterpene phytoalexins precursors biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ent-isokaurene,NADPH:oxygen oxidoreductase (hydroxylating)
This is the initial step in the conversion of ent-isokaurene to the antibacterial oryzalides in rice, Oryza sativa.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme acts as a C2-hydroxylase involved in a latter step of phytocassane biosynthesis
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-isokaurene + O2 + NADPH + H+
ent-2alpha-hydroxyisokaurene + H2O + NADP+
show the reaction diagram
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ir
additional information
?
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the enzyme does not react with ent-cassadiene
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
ent-isokaurene
-
pH and temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
ent-isokaurene
Oryza sativa
-
pH and temperature not specified in the publication
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 cells
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.143)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)