Information on EC 1.14.13.116 - geranylhydroquinone 3''-hydroxylase

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The expected taxonomic range for this enzyme is: Lithospermum erythrorhizon

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EC NUMBER
COMMENTARY hide
1.14.13.116
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RECOMMENDED NAME
GeneOntology No.
geranylhydroquinone 3''-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylhydroquinone + NADPH + H+ + O2 = 3''-hydroxygeranylhydroquinone + NADP+ + H2O
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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-
shikonin biosynthesis
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Ubiquinone and other terpenoid-quinone biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
geranylhydroquinone,NADPH:oxygen oxidoreductase (3''-hydroxylating)
Contains cytochrome P-450.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Sieb. et Zucc.
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylhydroquinone + NADPH + H+ + O2
3''-hydroxygeranylhydroquinone + NADP+ + H2O
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylhydroquinone + NADPH + H+ + O2
3''-hydroxygeranylhydroquinone + NADP+ + H2O
show the reaction diagram
-
likely to be involved in shikonin and dihydroechinofuran biosynthesis
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-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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required, could not be replaced by NADH, FAD or FMN. The optimal concentration of NADPH for this reaction is between 1 and 5 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ancymidol
-
1 mM, 38% inhibition
CO
-
inhibitory effect of CO is reversed by illumination
cytochrome c
-
0.01 mM, 96% inhibition
ketoconazole
-
1 mM, 62% inhibition
Metyrapone
-
1 mM, 14% inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P-450
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involved in activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0015
geranylhydroquinone
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pH 7.4, 30°C, microsomal fraction
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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activity in K-phosphate buffer at pH 7.4 is only 40% of that observed in Tris-HCl buffer
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 8.3
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50% of maximal activiyt at pH 6.7 and pH 8.3. Tris-Cl buffer
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of glycerol (20%, v/v) to the microsomal fraction before freezing results in the loss of 80% of the activity
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stable against freezing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 1 month, microsomal protein retains 82% of the original activity
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.116)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)