Information on EC 1.14.12.8 - 4-sulfobenzoate 3,4-dioxygenase

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The expected taxonomic range for this enzyme is: Comamonas testosteroni

EC NUMBER
COMMENTARY
1.14.12.8
-
RECOMMENDED NAME
GeneOntology No.
4-sulfobenzoate 3,4-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4-sulfobenzoate + NADH + H+ + O2 = 3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
4-toluenesulfonate degradation I
-
Microbial metabolism in diverse environments
-
Toluene degradation
-
SYSTEMATIC NAME
IUBMB Comments
4-sulfobenzoate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming)
A system, containing a reductase which is an iron-sulfur flavoprotein (FMN), an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4-sulfobenzoate 3,4-dioxygenase system
-
-
-
-
oxygenase, 4-sulfobenzoate di-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
122933-81-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Comamonas testosteroni PSB-4
PSB-4
-
-
Manually annotated by BRENDA team
Comamonas testosteroni T-2
T-2
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
-
-
-
-
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
-
-
-
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
-
the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
-
-
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
-
reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity
-
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
Comamonas testosteroni PSB-4, Comamonas testosteroni T-2
-
-
-
-
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
Comamonas testosteroni T-2
-
the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
-
-
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
Comamonas testosteroni T-2
-
-, reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity
-
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
Comamonas testosteroni TER-1:
-
-
-
-
?
4-toluenesulfonate + NADH + O2
?
show the reaction diagram
-
the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
-
-
?
4-toluenesulfonate + NADH + O2
?
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni PSB-4, Comamonas testosteroni T-2
-
this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC)
-
-
?
4-toluenesulfonate + NADH + O2
?
show the reaction diagram
Comamonas testosteroni T-2
-
the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
-
-
?
4-toluenesulfonate + NADH + O2
?
show the reaction diagram
Comamonas testosteroni TER-1:
-
this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC)
-
-
?
additional information
?
-
Comamonas testosteroni, Comamonas testosteroni T-2
-
poor substrates: 2-sulfobenzoate, 3-sulfobenzoate, benzenesulfonate, 4-methylbenzenesulfonate, 4-hydroxybenzenesulfonate, 4-aminobenzenesulfonate, 4-nitrobenzenesulfonate, 4-chloro-benzenesulfonate, 4-sulfophenylacetate, 4-sulfophenylpropionate, 4-sulfophenylbutyrate, no substrates are: benzoate, 4-methylbenzoate, 4-hydroxybenzoate, 4-aminobenzoate, 4-nitrobenzoate, 4-chlorobenzoate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni T-2
-
reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
FMN
-
flavoprotein, 1 mol FMN/mol reductase B
additional information
-
no independent ferredoxin
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Fe2+
-
requirement, iron-sulfur protein, 1 mol (2Fe-2S) per mol reductase B, one 2Fe-2S center (Rieske-type) per oxygenase A subunit, atomic absorption spectroscopy
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.025
0.032
4-Sulfobenzoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.012
-
-
for enzyme system grown on sulfobenzoate
0.024
-
-
for enzyme system grown on toluenesulfonate
0.03
-
-
for enzyme system grown on sulfobenzoate
0.11
-
-
reductase B
0.8
-
-
oxygenase A
84
-
-
reductase B, dichlorophenolindophenol
138
-
-
reductase B, cytochrome c
318
-
-
reductase B, ferricyanide
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at, reductase B
30
-
-
assay at
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Comamonas testosteroni T-2
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
39000
-
-
reductase B, contains 1 mol of FMN and about 2 mol each of iron and inorganic sulfur per mol, Superose gel filtration
47000
-
-
reductase B, Sephadex G-2000 gel filtration
50000
-
-
dioxygenase A, SDS-PAGE
85000
-
-
oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Superose gel filtration
105000
-
-
oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Sephadex G-2000 gel filtration
additional information
-
-
simple multi-component dioxygenase of two, perhaps three components: reductase B coupled to dimeric oxygenase, reductase C exhibits only 15% of total activity
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 50000, oxygenase A, SDS-PAGE
dimer
Comamonas testosteroni T-2
-
2 * 50000, oxygenase A, SDS-PAGE
-
monomer
-
1 * 36000, reductase B, SDS-PAGE
monomer
Comamonas testosteroni T-2
-
1 * 36000, reductase B, SDS-PAGE
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ammonium sulfate precipitation leads to excessive loss of activity
-
chloride-buffers result in diminished yields during purification
-
glycerol, 30% v/v, stabilizes
-
repeated freezing and thawing inactivates
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purification of the oxygenase system
-