Information on EC 1.14.12.8 - 4-sulfobenzoate 3,4-dioxygenase

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The expected taxonomic range for this enzyme is: Comamonas testosteroni

EC NUMBER
COMMENTARY hide
1.14.12.8
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RECOMMENDED NAME
GeneOntology No.
4-sulfobenzoate 3,4-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-sulfobenzoate + NADH + H+ + O2 = 3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
-
-
-
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reduction
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-toluenesulfonate degradation I
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Microbial metabolism in diverse environments
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Toluene degradation
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SYSTEMATIC NAME
IUBMB Comments
4-sulfobenzoate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming)
A system, containing a reductase which is an iron-sulfur flavoprotein (FMN), an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+.
CAS REGISTRY NUMBER
COMMENTARY hide
122933-81-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PSB-4
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
4-toluenesulfonate + NADH + O2
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
-
flavoprotein, 1 mol FMN/mol reductase B
additional information
-
no independent ferredoxin
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025 - 0.032
4-Sulfobenzoate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.012
-
for enzyme system grown on sulfobenzoate
0.024
-
for enzyme system grown on toluenesulfonate
0.03
-
for enzyme system grown on sulfobenzoate
0.11
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reductase B
0.8
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oxygenase A
84
-
reductase B, dichlorophenolindophenol
138
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reductase B, cytochrome c
318
-
reductase B, ferricyanide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at, reductase B
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
-
reductase B, contains 1 mol of FMN and about 2 mol each of iron and inorganic sulfur per mol, Superose gel filtration
47000
-
reductase B, Sephadex G-2000 gel filtration
50000
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dioxygenase A, SDS-PAGE
85000
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oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Superose gel filtration
105000
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oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Sephadex G-2000 gel filtration
additional information
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simple multi-component dioxygenase of two, perhaps three components: reductase B coupled to dimeric oxygenase, reductase C exhibits only 15% of total activity
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation leads to excessive loss of activity
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chloride-buffers result in diminished yields during purification
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glycerol, 30% v/v, stabilizes
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repeated freezing and thawing inactivates
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification of the oxygenase system
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