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Information on EC 1.14.11.24 - 2'-deoxymugineic-acid 2'-dioxygenase and Organism(s) Hordeum vulgare and UniProt Accession Q40062

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IUBMB Comments
Requires iron(II). It is also likely that this enzyme can catalyse the hydroxylation of 3-epihydroxy-2'-deoxymugineic acid to form 3-epihydroxymugineic acid.
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This record set is specific for:
Hordeum vulgare
UNIPROT: Q40062
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The taxonomic range for the selected organisms is: Hordeum vulgare
The enzyme appears in selected viruses and cellular organisms
Synonyms
IDS3, mugineicacid synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mugineicacid synthase
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
2'-deoxymugineic acid,2-oxoglutarate:oxygen oxidoreductase (2-hydroxylating)
Requires iron(II). It is also likely that this enzyme can catalyse the hydroxylation of 3-epihydroxy-2'-deoxymugineic acid to form 3-epihydroxymugineic acid.
CAS REGISTRY NUMBER
COMMENTARY hide
133758-62-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxymugineic acid + 2-oxoglutarate + O2
mugineic acid + succinate + CO2
show the reaction diagram
-
-
-
?
3-epihydroxy-2'-deoxymugineic acid + 2-oxoglutarate + O2
? + succinate + CO2
show the reaction diagram
-
-
-
?
2'-deoxymugineic acid + 2-oxoglutarate + O2
mugineic acid + succinate + CO2
show the reaction diagram
-
-
-
-
?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
L. cv. Ehimehadaka
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
concomitant introduction of the Glycine max ferritin gene and Hordeum vulgare mugineic acid biosynthetic genes effectively increases the seed iron level without causing iron sensitivity under iron-limited conditions in Oryza sativa, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IDS3_HORVU
339
0
37732
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37700
x * 37700, calculation from nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 37700, calculation from nucleotide sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
introduction of Glycine max ferritin gene driven by two endosperm-specific promoters, along with Hordeum vulgare nicotianamine synthase gene HvNAS, two nicotianamine aminotransferase genes HvNAAT-A and -B, and mugineic acid synthase gene IDS3 to enhance mugineic acid production in Oryza sativa sp. japonica cv. Tsukinohikari plant seeds. Lines expressing both ferritin and mugineic acid biosynthetic genes show signs of iron-deficiency tolerance in calcareous soil, phenotypes, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
introduction of the Ids3 gene into Oryza sativa cultivar Nipponbare and Tsukinohikari which lack Ids3 homologues
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakanishi, H.; Okumura, N.; Emehara, Y.; Nishizawa, N.K.; Chino, M.; Mori, S.
Expression of a gene specific for iron deficiency (Ids3) in the root of Hordeum vulgare
Plant Cell Physiol.
34
401-410
1993
Hordeum vulgare
Manually annotated by BRENDA team
Nakanishi, H.; Yamaguchi, H.; Sasakuma, T.; Nishizawa, N.K.; Mori, S.
Two dioxygenase genes, Ids3 and Ids2, from Hordeum vulgare are involved in the biosynthesis of mugineic acid family phytosiderophores
Plant Mol. Biol.
44
199-207
2000
Hordeum vulgare (Q40062)
Manually annotated by BRENDA team
Kobayashi, T.; Nakanishi, H.; Takahashi, M.; Kawasaki, S.; Nishizawa, N.K.; Mori, S.
In vivo evidence that Ids3 from Hordeum vulgare encodes a dioxygenase that converts 2'-deoxymugineic acid to mugineic acid in transgenic rice
Planta
212
864-871
2001
Hordeum vulgare
Manually annotated by BRENDA team
Masuda, H.; Kobayashi, T.; Ishimaru, Y.; Takahashi, M.; Aung, M.S.; Nakanishi, H.; Mori, S.; Nishizawa, N.K.
Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene
Front. Plant Sci.
4
132
2013
Hordeum vulgare
Manually annotated by BRENDA team