enzyme maintains approximately 75% of its highest activity at a broad range from 20°C to 37°C. The activity decreases rapidly when the temperature is higher than 50°C
high MIOX2 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview
high MIOX1 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview
high MIOX4 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview
high MIOX5 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview
the quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. Except for MIOX2, transcripts for the other three MIOX genes are still detected at a level of 2-14% of their abundance in the wild-type, the mutant does not show any visible phenotype and produced viable pollen, but the incorporation of myo-inositol-derived sugars into cell walls is strongly inhibited by over 90% with no change in the ultrastructure of syncytial cell walls
the enzyme myo-inositol oxygenase is the key enzyme of a pathway leading from myo-inositol to UDP-glucuronic acid. The primary function of myo-inositol oxygenase for syncytium development after infection with Heterodera schachtii is probably not the production of UDP-glucuronic acid as a precursor for cell wall polysaccharides, but the reduction of myo-inositol levels and thereby a reduction in the galactinol level to avoid the induction of defence-related genes
a quadruple (miox1/2/4/5) mutant that incorporates T-DNA insertions in all four MIOX genes is generated. This mutant shows a severe reduction in transcripts for all four MIOX genes. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1
loss-of-function mutants in MIOX genes contain alterations in myo-inositol levels and growth changes in the root. Miox2 mutants can be complemented with a MIOX2:green fluorescent protein fusion
the quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. Except for MIOX2, transcripts for the other three MIOX genes are still detected at a level of 2-14% of their abundance in the wild-type, the mutant does not show any visible phenotype and produced viable pollen, but the incorporation of myo-inositol-derived sugars into cell walls is strongly inhibited by over 90% with no change in the ultrastructure of syncytial cell walls
the T-DNA insertion mutant atmiox1 is sensitive to alkaline stress, the phenotype can be complemented by expression of Glycine soja MIOX1 under constitutive CaMV35S promoter control
the enzyme myo-inositol oxygenase is the key enzyme of a pathway leading from myo-inositol to UDP-glucuronic acid. The primary function of myo-inositol oxygenase for syncytium development after infection with Heterodera schachtii is probably not the production of UDP-glucuronic acid as a precursor for cell wall polysaccharides, but the reduction of myo-inositol levels and thereby a reduction in the galactinol level to avoid the induction of defence-related genes
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants
generation of an AtMIOX1 gene T-DNA insertion mutant atmiox1, the AtMIOX1 gene is efficiently silenced by the T-DNA insertion in the atmiox1 mutant. Constitutive overepression of Glycine soja MIOX1 under constitutive CaMV35S promoter control can recover the phenotype leading to alkaline stress tolerance in the atmiox1 mutant plants, overview
Teng, F.; You, R.; Hu, M.; Liu, W.; Wang, L.; Tao, Y.
Production of D-glucuronic acid from myo-inositol using Escherichia coli whole-cell biocatalyst overexpressing a novel myo-inositol oxygenase from Thermothelomyces thermophile