Information on EC 1.13.12.19 - 2-oxoglutarate dioxygenase (ethylene-forming)

New: Word Map on EC 1.13.12.19
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Pseudomonas syringae

EC NUMBER
COMMENTARY hide
1.13.12.19
-
RECOMMENDED NAME
GeneOntology No.
2-oxoglutarate dioxygenase (ethylene-forming)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-oxoglutarate + O2 = ethylene + 3 CO2 + H2O
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ethylene biosynthesis II (microbes)
-
-
ethylene biosynthesis IV (engineered)
-
-
ethylene biosynthesis V (engineered)
-
-
ethylene forming enzyme
-
-
SYSTEMATIC NAME
IUBMB Comments
2-oxoglutarate:oxygen oxidoreductase (decarboxylating, ethylene-forming)
This is one of two simultaneous reactions catalysed by the enzyme, which is responsible for ethylene production in bacteria of the Pseudomonas syringae group. In the other reaction [EC 1.14.11.34, 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)] the enzyme catalyses the mono-oxygenation of both 2-oxoglutarate and L-arginine, forming succinate, carbon dioxide and L-hydroxyarginine, which is subsequently cleaved into guanidine and (S)-1-pyrroline-5-carboxylate. The enzymes catalyse two cycles of the ethylene-forming reaction for each cycle of the succinate-forming reaction, so that the stoichiometry of the products ethylene and succinate is 2:1.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate + O2
ethylene + 3 CO2 + H2O
show the reaction diagram
2-oxoglutarate + O2
ethylene + ?
show the reaction diagram
presence of oxygen is essential for the ethylene forming reaction by EFE
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4,5-dihydroxy-1,3-benzene disulfonic acid
1 mM, 0.8% residual activity
5,5'-dithio-bis(2-nitrobenzoate)
1 mM, 0.7% residual activity
CoCl2
1 mM, 20% residual activity
CuSO4
1 mM, 50% residual activity
EDTA
1 mM, 1% residual activity
H2O2
1 mM, 0.7% residual activity
MnCl2
1 mM, 6% residual activity
n-propyl gallate
1 mM, 1% residual activity
Sodium azide
1 mM, 90% residual activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-arginine
3% of the activity with L-arginine
L-arginine
highly specific for cofactor L-arginine, KM value 0.018 mM
L-canavanine sulfate
7% of the activity with L-arginine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006 - 0.033
2-oxoglutarate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009 - 0.5
2-oxoglutarate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.27 - 38.5
2-oxoglutarate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9
isoelectric focusing
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from cell-free extract
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisae. Different cultivation factors on ethylene formation in Saccharomyces cerevisiae expressing the EFE in continuous cultures are investigated. Main finding is that oxygen availability is crucial for ethylene production. By employing three different nitrogen sources it is shown that the nitrogen source available can both improve and impair the ethylene productivity. N-Source/yield ethylene (microgram/g glucose): (NH4)2SO4/164, glutamate/233, glutamate+arginine/96.8
-
expression in Escherichia coli
-
expression in Nicotiana tabacum
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H116Q
-
kcat value decreases to 2.4% of wild-type. Mutant is more thermolabile than wild-type
H168Q
-
kcat value decreases to 3% of wild-type. Mutant is more thermolabile than wild-type
H169Q
-
kcat value decreases to 9.3% of wild-type. Mutant is more thermolabile than wild-type
H189Q
-
complete loss of activity
H233Q
-
complete loss of activity
H268Q
-
kcat value decreases to 1.8% of wild-type
H284Q
-
kcat value decreases to 2% of wild-type. Mutant is more thermolabile than wild-type
H305Q
-
kcat value decreases to 40% of wild-type
H309Q
-
kcat value decreases to 3.3% of wild-type. Mutant is more thermolabile than wild-type
H335Q
-
kcat value decreases to 60% of wild-type
additional information
-
introduction of a gene encoding a chimeric protein consisting of EFE and beta-glucuronidase GUS into the tobacco genome using a binary vector which directs expression of the EFE-GUS fusion protein under the control of constitutive promoter of cauliflower mosaic virus 35S RNA
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
introduction of a gene encoding a chimeric protein consisting of EFE and beta-glucuronidase GUS into the tobacco genome using a binary vector which directs expression of the EFE-beta-glucuronidase fusion protein under the control of constitutive promoter of cauliflower mosaic virus 35S RNA. Transgenic plants produce ethylene at consistently higher rates than the untransformed plant, and their beta-glucuronidase activities are expressed in different tissues. A significant dwarf morphology observed in the transgenic tobacco displaying the highest ethylene production resembles the phenotype of a wild-type plant exposed to excess ethylene
biotechnology
-
different cultivation factors on ethylene formation in Saccharomyces cerevisiae expressing the EFE in continuous cultures is investigated. Main finding is that oxygen availability is crucial for ethylene production. By employing three different nitrogen sources it is shown that the nitrogen source available can both improve and impair the ethylene productivity
Show AA Sequence (144 entries)
Please use the Sequence Search for a certain query.