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Information on EC 1.13.11.6 - 3-hydroxyanthranilate 3,4-dioxygenase and Organism(s) Pseudomonas fluorescens and UniProt Accession Q83V26

for references in articles please use BRENDA:EC1.13.11.6
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IUBMB Comments
Requires Fe2+.
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This record set is specific for:
Pseudomonas fluorescens
UNIPROT: Q83V26
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The taxonomic range for the selected organisms is: Pseudomonas fluorescens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilic acid oxygenase, 3-hao, 3-hydroxyanthranilate oxygenase, 3-had, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxyanthranilate oxygenase
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3-hydroxyanthranilic acid oxidase
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3-hydroxyanthranilic acid oxygenase
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3-hydroxyanthranilic oxygenase
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3HAO
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oxygenase, 3-hydroxyanthranilate 3,4-di-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
3-hydroxyanthranilate:oxygen 3,4-oxidoreductase (decyclizing)
Requires Fe2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-50-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxyanthranilate + O2
2-amino-3-carboxymuconate semialdehyde
show the reaction diagram
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-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxyanthranilate + O2
2-amino-3-carboxymuconate semialdehyde
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
needed for catalytic activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
Fe2+ chelator, 1 mM, complete inhibition
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide methiodide
carboxyl-directed reagent, 1 mM, 26% inhibition
2,2'-dipyridyl
Fe2+ chelator, 1 mM, complete inhibition
Cd2+
0.1 mM, more than 99% inhibition
Co2+
1 mM, 92% inhibition
Cu2+
0.1 mM, complete inhibition
diethyl dicarbonate
modifies histidine residues of catechol dioxygenases, 1 mM, 70% inhibition
Dithionitrobenzoic acid
cysteine-directed reagent, 1 mM, complete inhibition
EDTA
1 mM, 99% inhibition
Fe3+
0.1 mM, 82% inhibition
iodoacetate
cysteine-directed reagent, 1 mM, 42% inhibition
Ni2+
1 mM, 82% inhibition
Zn2+
0.1 mM, 99% inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.9
3-hydroxyanthranilate as substrate, pH 6.5, 30ºC
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
3HAO_PSEFL
185
0
21245
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21240
predicted from cDNA sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H52A
inactive enzyme
H93A
24.8% activity of the native enzyme
H96A
inactive enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Muraki, T.; Taki, M.; Hasegawa, Y.; Iwaki, H.; Lau, P.C.
Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7
Appl. Environ. Microbiol.
69
1564-1572
2003
Pseudomonas fluorescens (Q83V26), Pseudomonas fluorescens, Pseudomonas fluorescens KU-7 (Q83V26)
Manually annotated by BRENDA team