Information on EC 1.13.11.57 - gallate dioxygenase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
1.13.11.57
-
RECOMMENDED NAME
GeneOntology No.
gallate dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gallate + O2 = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
show the reaction diagram
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-
-
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PATHWAY
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
gallate degradation I
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
gallate:oxygen oxidoreductase
Contains non-heme Fe2+. The enzyme is a ring-cleavage dioxygenase that acts specifically on gallate to produce the keto-tautomer of 4-oxalomesaconate [1,2].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
DesB
Sphingobium sp.
-
-
DesB
Sphingomonas paucimobilis SYK-6
Q5NTE5
-
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GA dioxygenase
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GALA
-
-
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Sphingobium sp.
formerly Sphingomonas paucimobilis strain SYK-6
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Manually annotated by BRENDA team
formerly Sphingomonas paucimobilis strain SYK-6
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-
Manually annotated by BRENDA team
Sphingomonas paucimobilis SYK-6
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
Q5NTE5
the enzyme is involved in syringate catabolism, the pathway involving DesB plays an especially important role in the growth of Sphingomonas paucimobilis SYK-6 on syringate, and DesB is involved in gallate degradation
physiological function
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the GalA protein is the only dioxygenase involved in gallate cleavage in Pseudomonas putida KT2440
physiological function
Sphingomonas paucimobilis SYK-6
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the enzyme is involved in syringate catabolism, the pathway involving DesB plays an especially important role in the growth of Sphingomonas paucimobilis SYK-6 on syringate, and DesB is involved in gallate degradation
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SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
gallate + O2
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
show the reaction diagram
-
gallate is also namend gallic acid or 3,4,5-trihydroxybenzoic acid
-
-
?
gallate + O2
4-oxalomesaconate
show the reaction diagram
Sphingobium sp.
-
-
-
-
?
gallate + O2
4-oxalomesaconate
show the reaction diagram
Q5NTE5
-
-
-
?
gallate + O2
4-oxalomesaconate
show the reaction diagram
-
the substrate specificity is very restricted toward gallate
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-
?
gallate + O2
4-oxalomesaconate
show the reaction diagram
Sphingomonas paucimobilis SYK-6
Q5NTE5
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-
-
?
pyrogallol + O2
?
show the reaction diagram
Sphingomonas paucimobilis, Sphingomonas paucimobilis SYK-6
Q5NTE5
-
-
-
?
gallate + O2
4-oxalomesaconate
show the reaction diagram
-
-
-
-
?
additional information
?
-
Q5NTE5
no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate
-
-
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additional information
?
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no activity with protocatechuate, catechol, resorcinolate, resorcinol, gentisate, hydroquinone, pyrogallol, methylgallate, or syringate
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-
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additional information
?
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Sphingomonas paucimobilis SYK-6
Q5NTE5
no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Fe2+
Q5NTE5
DesB requires Fe2+ for activity
Fe2+
-
required for activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,2'-dipyridyl
-
2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely
EDTA
Q5NTE5
complete inhibition at 0.5 mM, the oxygen consumption activity after EDTA treatment is completely recovered by addition of Fe2+ (119%)
EDTA
-
complete inhibition at 1 mM, the activity is only fully recovered with Fe2+
H2O2
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hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0669
-
gallate
Q5NTE5
in 50 mM GTA buffer (pH 8.5) consisting of 50 mM 3,3-dimethylglutarate, 50 mM Tris, and 50 mM 2-amino-2-methyl-1,3-propanediol, at 30C
0.144
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gallate
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in 100 mM Tris-HCl, pH 7.0, at 30C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
21.4
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crude extract, in 100 mM Tris-HCl, pH 7.0, at 30C
42.7
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after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
47700
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-
calculated from amino acid sequence
86000
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Q5NTE5
gel filtration
93800
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Sphingobium sp.
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calculated from amino acid sequence
132000
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molar mass obtained by sedimentation equilibrium
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
homodimer
Sphingobium sp.
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x-ray crystallography
homodimer
Q5NTE5
2 * 45000, SDS-PAGE; 2 * 46843, calculated from amino acid sequence
homodimer
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x-ray crystallography
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homodimer
Sphingomonas paucimobilis SYK-6
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2 * 45000, SDS-PAGE; 2 * 46843, calculated from amino acid sequence
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homotrimer
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3 * 47000, SDS-PAGE; 3 * 47644, calculated from amino acid sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging drop vapor diffusion method, using 25% (w/v) PEG 8000, 0.1 M sodium acetate trihydrate, 0.1 M HEPES-NaOH pH 7.8, at 4C
Sphingobium sp.
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ultracentrifugation and phenyl-Sepharose column chromatography
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POROS PI column chromatography and POROS HQ column chromatography
Sphingobium sp.
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POROS polyethyleneimine column chromatography
Q5NTE5
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) cells
Sphingobium sp.
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expressed in Escherichia coli BL21(DE3) cells
Q5NTE5
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the expression of the galA gene is specifically induced by gallate
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