Information on EC 1.13.11.57 - gallate dioxygenase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY hide
1.13.11.57
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RECOMMENDED NAME
GeneOntology No.
gallate dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
gallate + O2 = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminobenzoate degradation
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gallate degradation I
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
gallate:oxygen oxidoreductase
Contains non-heme Fe2+. The enzyme is a ring-cleavage dioxygenase that acts specifically on gallate to produce the keto-tautomer of 4-oxalomesaconate [1,2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Sphingobium sp.
formerly Sphingomonas paucimobilis strain SYK-6
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Manually annotated by BRENDA team
formerly Sphingomonas paucimobilis strain SYK-6
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
gallate + O2
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
show the reaction diagram
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gallate is also namend gallic acid or 3,4,5-trihydroxybenzoic acid
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?
gallate + O2
4-oxalomesaconate
show the reaction diagram
pyrogallol + O2
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
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2 mM 2,2'-dipyridyl, a specific Fe2+-chelator, abolishes the gallate dioxygenase activity completely
H2O2
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hydrogen peroxide at 1 mM reduces the gallate dioxygenase activity to 15%, and the activity can be partially restored (50% activity of the untreated enzyme) upon addition of 10 mM ascorbate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0669 - 0.144
gallate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21.4
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crude extract, in 100 mM Tris-HCl, pH 7.0, at 30C
42.7
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after 2fold purification, in 100 mM Tris-HCl, pH 7.0, at 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47700
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calculated from amino acid sequence
86000
gel filtration
93800
Sphingobium sp.
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calculated from amino acid sequence
132000
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molar mass obtained by sedimentation equilibrium
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
homotrimer
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3 * 47000, SDS-PAGE; 3 * 47644, calculated from amino acid sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 25% (w/v) PEG 8000, 0.1 M sodium acetate trihydrate, 0.1 M HEPES-NaOH pH 7.8, at 4C
Sphingobium sp.
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
POROS PI column chromatography and POROS HQ column chromatography
Sphingobium sp.
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POROS polyethyleneimine column chromatography
ultracentrifugation and phenyl-Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression of the galA gene is specifically induced by gallate
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