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Information on EC 1.13.11.51 - 9-cis-epoxycarotenoid dioxygenase and Organism(s) Zea mays and UniProt Accession O24592

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IUBMB Comments
Requires iron(II). Acts on 9-cis-violaxanthin and 9'-cis-neoxanthin but not on the all-trans isomers [2,3]. In vitro, it will cleave 9-cis-zeaxanthin. Catalyses the first step of abscisic-acid biosynthesis from carotenoids in chloroplasts, in response to water stress. The other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic-aldehyde oxidase) and EC 1.14.13.93 [(+)-abscisic acid 8'-hydroxylase].
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Zea mays
UNIPROT: O24592
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Word Map
The taxonomic range for the selected organisms is: Zea mays
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
9-cis-epoxycarotenoid dioxygenase, nced3, atnced3, nced1, nced2, nced5, nced9, lenced1, sgnced1, nced4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9-cis epoxycarotenoid dioxygenase
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AtNCED3
-
nine-cis-epoxycarotenoid dioxygenase
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PvNCED1
-
VP14 protein
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VP14
-
-
VP14 epoxy-carotenoid dioxygenase
-
-
SYSTEMATIC NAME
IUBMB Comments
9-cis-epoxycarotenoid 11,12-dioxygenase
Requires iron(II). Acts on 9-cis-violaxanthin and 9'-cis-neoxanthin but not on the all-trans isomers [2,3]. In vitro, it will cleave 9-cis-zeaxanthin. Catalyses the first step of abscisic-acid biosynthesis from carotenoids in chloroplasts, in response to water stress. The other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic-aldehyde oxidase) and EC 1.14.13.93 [(+)-abscisic acid 8'-hydroxylase].
CAS REGISTRY NUMBER
COMMENTARY hide
199877-10-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9'-cis-neoxanthin + O2
?
show the reaction diagram
-
-
-
-
?
9-cis-8'R-luteoxanthin + O2
?
show the reaction diagram
-
-
-
-
?
9-cis-8'S-luteoxanthin + O2
?
show the reaction diagram
-
-
-
-
?
9-cis-antheraxanthin + O2
?
show the reaction diagram
-
-
-
-
?
9-cis-violaxanthin + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
9'-cis-neoxanthin
-
-
0.008
9-cis-8'R-luteoxanthin
-
-
0.015
9-cis-8'S-luteoxanthin
-
-
0.02
9-cis-antheraxanthin
-
-
0.058
9-cis-violaxanthin
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
strongly induced by water stress
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
VP14 is imported into chloroplast with cleavage of a short stroma-targeting domain. Mature VP14 exists in two forms, one which is soluble in stroma and the other bound to thylakoid
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NCED1_MAIZE
604
0
65495
Swiss-Prot
Chloroplast (Reliability: 3)
PDB
SCOP
CATH
UNIPROT
ORGANISM
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
VP14 is imported into chloroplast with cleavage of a short stroma-targeting domain. Mature VP14 exists in two forms, one of which is soluble in stroma and the other bound to thylakoid
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schwartz, S.H.; Tan, B.C.; McCarty, D.R.; Welch, W.; Zeevaart, J.A.
Substrate specificity and kinetics for VP14, a carotenoid cleavage dioxygenase in the ABA biosynthetic pathway
Biochim. Biophys. Acta
1619
9-14
2003
Zea mays
Manually annotated by BRENDA team
Tan, B.C.; Cline, K.; McCarty, D.R.
Localization and targeting of the VP14 epoxy-carotenoid dioxygenase to chloroplast membranes
Plant J.
27
373-382
2001
Zea mays
Manually annotated by BRENDA team
Tan, B.C.; Schwartz, S.H.; Zeevaart, J.A.; McCarty, D.R.
Genetic control of abscisic acid biosynthesis in maize
Proc. Natl. Acad. Sci. USA
94
12235-12240
1997
Zea mays (O24592), Zea mays
Manually annotated by BRENDA team
Schwartz, S.H.; Tan, B.C.; Gage, D.A.; Zeevaart, J.A.; McCarty, D.R.
Specific oxidative cleavage of carotenoids by VP14 of maize
Science
276
1872-1874
1997
Zea mays (O24592), Zea mays
Manually annotated by BRENDA team
Guerrini, I.A.; Trigueiro, R.M.; Leite, R.M.; Wilcken, C.F.; Velini, E.D.; Mori, E.S.; Furtado, E.L.; Marino, C.L.; Maia, I.G.
Eucalyptus ESTs involved in the production of 9-cis epoxycarotenoid dioxygenase, a regulatory enzyme of abscisic acid production
Genet. Mol. Biol.
28
640-643
2005
Arabidopsis thaliana, Eucalyptus globulus, Eucalyptus camaldulensis, Eucalyptus grandis, Eucalyptus saligna, Eucalyptus urophylla, Solanum lycopersicum (O24023), Zea mays (O24592), Persea americana (Q9AXZ3), Vigna unguiculata (Q9FS24), Solanum tuberosum (Q9M3Z9), Phaseolus vulgaris (Q9M6E8)
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Manually annotated by BRENDA team