Information on EC 1.13.11.37 - hydroxyquinol 1,2-dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
1.13.11.37
-
RECOMMENDED NAME
GeneOntology No.
hydroxyquinol 1,2-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
benzene-1,2,4-triol + O2 = maleylacetate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
2,4,5-trichlorophenoxyacetate degradation
-
4-aminophenol degradation
-
4-nitrophenol degradation II
-
Benzoate degradation
-
Chlorocyclohexane and chlorobenzene degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
resorcinol degradation
-
resorcinol degradation
BRENDA
BRENDA
BRENDA
SYSTEMATIC NAME
IUBMB Comments
benzene-1,2,4-triol:oxygen 1,2-oxidoreductase (decyclizing)
An iron protein. Highly specific; catechol and pyrogallol are acted on at less than 1% of the rate at which hydroxyquinol is oxidized.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,2-HQD
Q5PXQ6
-
1,2-HQD
Pimelobacter simplex 3E
Q5PXQ6
-
-
hydroxyquinol 1,2-dioxygenase
-
-
hydroxyquinol 1,2-dioxygenase
-
-
hydroxyquinol 1,2-dioxygenase
-
-
-
hydroxyquinol-1,2-dioxygenase
-
-
hydroxyquinol-1,2-dioxygenase
Pseudomonas sp. HS-D38
-
-
-
NpcC
Rhodococcus opacus SAO101
-
-
-
oxygenase, hydroxyquinol 1,2-di-
-
-
-
-
oxygenase, hydroxyquinol di-
-
-
-
-
PdcC
-
gene name
PdcC
-
gene name
-
PnpC
-
gene name
PnpC
Pseudomonas sp. HS-D38
-
gene name
-
hydroxyquinon dioxygenase
-
-
-
-
additional information
Q3BEM2
cphA-1 encode hydroxyquinol 1,2-dioxygenases. The amino acid sequence of CphA-2 is 78.4% identical to that of CphA-1.
additional information
Q3BEN0
cphA-2 encodes hydroxyquinol 1,2-dioxygenases. The amino acid sequence of CphA-2 is 78.4% identical to that of CphA-1.
CAS REGISTRY NUMBER
COMMENTARY
91847-14-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain A6, DSM12829T
-
-
Manually annotated by BRENDA team
BA-5-17, grown in benzamide medium
SwissProt
Manually annotated by BRENDA team
Azotobacter sp.
strain GP1, DSM 6428, ATCC 49806, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
Azotobacter sp. GP1
strain GP1, DSM 6428, ATCC 49806, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
AC1100, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
L. ex Fr., 69346, grown with cellobiose
-
-
Manually annotated by BRENDA team
Fomes pinicola
Sw. ex Fr., Karst. B.H., grown with cellobiose
-
-
Manually annotated by BRENDA team
f. sp. vasinfectum
-
-
Manually annotated by BRENDA team
Lenzites trabea
Pers. ex Fr., Fr. Karst. A-576, grown with cellobiose
-
-
Manually annotated by BRENDA team
Pimelobacter simplex 3E
3E
SwissProt
Manually annotated by BRENDA team
Pimelobacter simplex 3E
strain 3E
-
-
Manually annotated by BRENDA team
Polyporus dichrous
Fr., grown with cellulose
-
-
Manually annotated by BRENDA team
Poria ambigua
Bres. BB3-1, grown with cellulose
-
-
Manually annotated by BRENDA team
gene pnpC
-
-
Manually annotated by BRENDA team
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA
-
-
Manually annotated by BRENDA team
strain PDS-7
-
-
Manually annotated by BRENDA team
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA
-
-
Manually annotated by BRENDA team
Pseudomonas sp. HS-D38
gene pnpC
-
-
Manually annotated by BRENDA team
strain PDS-7
-
-
Manually annotated by BRENDA team
strain SAO101
-
-
Manually annotated by BRENDA team
Rhodococcus opacus SAO101
strain SAO101
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
grown in presence of vanillic acid
-
-
Manually annotated by BRENDA team
strain 303, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
Streptomyces rochei 303
strain 303, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
Jacq. ex Fr., Karst. A-360, grown with cellulose
-
-
Manually annotated by BRENDA team
grown in presence of L-tyrosine
-
-
Manually annotated by BRENDA team
grown with hydrobenzoate
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol in the route via 4-nitrocatechol, overview
metabolism
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol inthe route via 4-nitrocatechol, overview
metabolism
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol inthe route via 4-nitrocatechol, overview
-
metabolism
Pseudomonas sp. HS-D38
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol in the route via 4-nitrocatechol, overview
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
-
-
-
?
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
Azotobacter sp.
-
33.5% relative activity of that measured with hydroxyquinol
-
?
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
Streptomyces rochei 303
-
-
-
?
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
Azotobacter sp. GP1
-
33.5% relative activity of that measured with hydroxyquinol
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Azotobacter sp.
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Fomes pinicola, Lenzites trabea, Poria ambigua, Daedalea quercina, Polyporus dichrous
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Q9S1B4
6.8-fold activity of that for catechol
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Streptomyces rochei 303
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Pseudomonas sp. HS-D38
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Azotobacter sp. GP1
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Burkholderia cepacia AC1100
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp.
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Fomes pinicola, Lenzites trabea, Poria ambigua, Daedalea quercina, Polyporus dichrous
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Q9S1B4
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Streptomyces rochei 303
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp. GP1
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Burkholderia cepacia AC1100
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Q5PXQ6
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Rhodococcus opacus SAO101
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Pimelobacter simplex 3E
Q5PXQ6
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
cis,cis-muconic acid
show the reaction diagram
-
-
-
-
?
catechol + O2
cis,cis-muconic acid
show the reaction diagram
Q9S1B4
intradiol ring cleavage
-
?
catechol + O2
2-hydroxymuconic 6-semialdehyde
show the reaction diagram
Q9S1B4
extradiol ring cleavage
-
?
hydroxyquinol + O2
?
show the reaction diagram
-
-
-
-
?
hydroxyquinol + O2
maleylacetate
show the reaction diagram
-
intradiol ringcleavage
-
-
?
additional information
?
-
-, Q3BEM2, Q3BEN0
CphA-1 exhibits no activity with 4-chlorocatechol, hydroquinone, or resorcinol
-
-
-
additional information
?
-
-, Q3BEM2, Q3BEN0
CphA-2 exhibits no activity with 4-chlorocatechol, hydroquinone, or resorcinol
-
-
-
additional information
?
-
-
Pseudomonas sp. PDS-7 is capable of utilizing p-nitrophenol as the sole carbon, nitogen and energy source, concentration up to 80 mg/l
-
-
-
additional information
?
-
-
when the cells are grown on a mixture of 4-chlorophenol, 4-nitrophenol and phenol, 4-chlorophenol degradation apparently is delayed until 4-nitrophenol is almost completely depleted. Phenol is degraded more slowly than the other compounds and not until 4-nitrophenol and 4-chlorophenol are depleted, despite this being the least toxic compound of the three
-
-
-
additional information
?
-
-
PnpG is not active against protocatechuate and gentisate
-
-
-
additional information
?
-
-
Pseudomonas sp. PDS-7 is capable of utilizing p-nitrophenol as the sole carbon, nitogen and energy source, concentration up to 80 mg/l
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp.
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Fomes pinicola, Lenzites trabea, Poria ambigua, Daedalea quercina, Polyporus dichrous
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Q9S1B4
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Q5PXQ6
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Streptomyces rochei 303
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Pimelobacter simplex 3E
Q5PXQ6
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Pseudomonas sp. HS-D38
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp. GP1
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Burkholderia cepacia AC1100
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
stimulates at 0.2 mM
Cu2+
-
stimulates at 0.2 mM
Fe2+
-
stimulates at 0.2 mM
Fe3+
-
catalytically essential
Fe3+
Q5PXQ6
required for activity
Fe3+
-
stimulates at 0.2 mM
Iron
Azotobacter sp.
-
0.18 mol per mol enzyme
Iron
Azotobacter sp.
-
1 mol of iron per mol enzyme
Iron
-
1 mol of iron per mol enzyme
Mn2+
-
stimulates at 0.2 mM
Zn2+
-
stimulates at 0.2 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,4-Dichlorophenol
Azotobacter sp.
-
mixed inhibition
2,4-Dichlorophenol
-
competitive inhibition
2,6-dichlorohydroquinone
Azotobacter sp.
-
competitive nhibition
2,6-dichlorohydroquinone
-
competitive inhibition
3,4,6-trichlorocatechol
Azotobacter sp., Streptomyces rochei
-
mixed inhibition
3,5-dichlorocatechol
Azotobacter sp.
-
competitive inhibition
3,5-dichlorocatechol
-
mixed inhibition
3-chlorocatechol
Azotobacter sp.
-
competitive inhibition
3-chlorocatechol
-
mixd inhibition
3-methyl-5-chlorocatechol
Azotobacter sp.
-
competitive inhibition
3-methyl-5-chlorocatechol
-
mixed inhibition
4,5-dichlorocatechol
Azotobacter sp.
-
competitive inhibition
4,5-dichlorocatechol
-
parially mixed competitive inhibition
4-Chlorocatechol
Azotobacter sp., Streptomyces rochei
-
competitive inhibition
catechol
Azotobacter sp.
-
mixed inhibition
EDTA
Azotobacter sp.
-
slight inhibition
monochlorohydroquinone
Azotobacter sp., Streptomyces rochei
-
competitive inhibition
pyrogallol
Azotobacter sp.
-
mixed inhibition
tetrachlorocatechol
Azotobacter sp., Streptomyces rochei
-
mixed inhibition
Tetrachlorohydroquinone
Azotobacter sp., Streptomyces rochei
-
competitive inhibition
Tiron
Azotobacter sp.
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Fe(NH4)2(SO4)2
Azotobacter sp.
-
0.00001-0.000001 mM
Fe(NH4)2(SO4)2
Azotobacter sp.
-
no activation by Fe2+ ions
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.06
-
6-chlorohydroxyquinol
-
-
0.0012
-
Benzene-1,2,4-triol
Q5PXQ6
-
0.000035
-
Hydroxyquinol
Sporotrichum pulverulentum
-
-
0.0057
-
Hydroxyquinol
-
-
0.007
-
Hydroxyquinol
-
-
0.02
-
Hydroxyquinol
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
72.3
-
6-chlorohydroxyquinol
-
-
29
-
Benzene-1,2,4-triol
Q5PXQ6
-
6.35
-
Hydroxyquinol
-
-
11.4
-
Hydroxyquinol
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01274
-
2,4-Dichlorophenol
Azotobacter sp.
-
-
0.01719
-
2,4-Dichlorophenol
-
-
0.00589
-
2,6-dichlorohydroquinone
-
-
0.01094
-
2,6-dichlorohydroquinone
Azotobacter sp.
-
-
0.00536
-
3,4,6-trichlorocatechol
-
-
0.0112
-
3,4,6-trichlorocatechol
Azotobacter sp.
-
-
0.0088
-
3,5-dichlorocatechol
Azotobacter sp.
-
-
0.0169
-
3,5-dichlorocatechol
-
-
0.00698
-
3-chlorocatechol
Azotobacter sp.
-
-
0.03077
-
3-chlorocatechol
-
-
0.02732
-
3-methyl-5-chlorocatechol
Azotobacter sp.
-
-
0.0373
-
3-methyl-5-chlorocatechol
-
-
0.00585
-
4,5-dichlorocatechol
-
-
0.0103
-
4,5-dichlorocatechol
Azotobacter sp.
-
-
0.0073
-
4-Chlorocatechol
Azotobacter sp.
-
-
0.0526
-
4-Chlorocatechol
-
-
0.00956
-
catechol
Azotobacter sp.
-
-
10
-
catechol
Q5PXQ6
approximate value
0.00083
-
monochlorohydroquinone
-
-
0.00107
-
monochlorohydroquinone
Azotobacter sp.
-
-
0.00069
-
pyrogallol
Azotobacter sp.
-
-
0.00128
-
tetrachlorocatechol
-
-
0.00349
-
tetrachlorocatechol
Azotobacter sp.
-
-
0.00226
-
Tetrachlorohydroquinone
-
-
0.01248
-
Tetrachlorohydroquinone
Azotobacter sp.
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.37
-
-
substrate hydroxyquinol
0.45
-
-
substrate catechol
7.06
-
Azotobacter sp.
-
reactivated with 0.01 mM Fe2+
9.3
-
-
purified recombinant His-tagged enzyme, pH 5.0, 45C
additional information
-
Azotobacter sp.
-
-
additional information
-
Q9S1B4
-
additional information
-
-, Q3BEM2, Q3BEN0
cell extracts from 4-chlorophenol-grown cells deplete hydoxyquinol from the medium with an activity of 96 microM/min/mg protein. No depletion of hydroxyquinol is measured in extracts of Succinate-grown cells.; cell extracts from 4-chlorophenol-grown cells deplete hydoxyquinol from the medium with an activity of 96 microM/min/mg protein. No depletion of hydroxyquinol is measured in extracts of Succinate-grown cells.; Cph-2 remove hydroxyquinol: CphA-2 = 3.0 microM/min/mg. CphA-2 show some activity with catechol: CphA-2 = 0.15 microM/min/mg.; CphA-1 remove hydroxyquinol: CphA-1 = 16 microM/min/mg. CphA-1 show some activity with catechol: CphA-1 = 0.67 microM/min/mg.
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
-
enzyme assay
6.4
-
Azotobacter sp.
-
in succinate buffer
6.5
-
Sporotrichum pulverulentum
-
enzyme assay
8.5
-
Q9S1B4
for catechol
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
Sporotrichum pulverulentum
-
enzyme assay
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Sporotrichum pulverulentum
-
-
Manually annotated by BRENDA team
Trametes cinnabarina, Fomes pinicola, Lenzites trabea, Poria ambigua, Daedalea quercina, Polyporus dichrous
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
34800
-
-
estimated from SDS-PAGE
40000
-
-, Q3BEM2, Q3BEN0
recombinant CphA-1
42000
-
-, Q3BEM2, Q3BEN0
recombinant CphA-2
58000
-
Azotobacter sp.
-
gel filtration
61000
-
-
gel filtration
68000
-
-
gel filtration
79000
-
Q9S1B4
gel filtration
93000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Q9S1B4
? * 36000, SDS-PAGE
?
-
x * 33200, deduced from nucleotide sequence
?
-
x * 33000, about, sequence calculation
?
Rhodococcus opacus SAO101
-
x * 33200, deduced from nucleotide sequence
-
?
Pseudomonas sp. HS-D38
-
x * 33000, about, sequence calculation
-
dimer
-
2 * 36000, SDS-PAGE
dimer
Azotobacter sp.
-
2 * 34250, SDS-PAGE
dimer
Azotobacter sp. GP1
-
2 * 34250, SDS-PAGE
-
dimer
Burkholderia cepacia AC1100
-
2 * 36000, SDS-PAGE
-
heterodimer
-
alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE
homodimer
-
2 * 31000, SDS-PAGE
homodimer
-
two identical subunits in a alpha2-type quaternary strucure
homodimer
Streptomyces rochei 303
-
2 * 31000, SDS-PAGE
-
homodimer
Pimelobacter simplex 3E
-
two identical subunits in a alpha2-type quaternary strucure
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sitting drop vapor difffusion from a solution containing 2 M ammonium sulfate, 4% polyethylene glycol 400, 100 mM Hepes, pH 7.5, drops consist of 0.004 ml of 20 mg/ml protein solution and 0.006 ml reservoir solution equilibrated against 0.05 ml of reservoir solution, crystals diffract to 1.75 A
Q5PXQ6
unit-cell dimensions: a = 81.15, b = 86.79, c = 114.93
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
Q9S1B4
activity not influenced after incubation for 10 min
55
-
Q9S1B4
60% activity loss after 10 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, no loss of activity after several weeks, at room temperature no considerable decrease after 1 day
Azotobacter sp.
-
4C, stable for 2 to 3 weeks in elution buffer, can not be stored at 4C or frozen at -20C
-
4C, reasonably stable for several weeks in the presence of isopropanol
-
4C, loss of most of the activity after 1 week
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Azotobacter sp.
-
recombinant 1,2-HQD
Q5PXQ6
Ni-NTA column chromatography
-
recombinant His-tagged enzyme 37.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cphA-1 from Arthrobacter chloropenolicus A6 is cloned into the vectro pCRT7/CT-TOPO, the construct is transformed into CL21(DE3)/pLysS cells.; cphA-2 from Arthrobacter chloropenolicus A6 is cloned into the vectro pCRT7/CT-TOPO, the construct is transformed into CL21(DE3)/pLysS cells.
-, Q3BEM2, Q3BEN0
expressed in Escherichia coli
Q9S1B4
expressed in Escherichia coli
-
expression in Escherichia coli
Q5PXQ6
expressed in Escherichia coli BL21 cells
-
expression in Escherichia coli BL21
-
gene pnpC, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-, Q3BEM2, Q3BEN0
Arthrobacter chlorophenolicus A6 is transformed with plasmid pKGT452Cbeta, which contain an Arthrobacter-derived, randomly inserting transposon conferring chloramphenicol resistance. The transposon mutagenese to disrupt the cphA-1 gene generates mutant strain Arthrobacter chlorophenolicus T99. The T99 mutant is not able to grow on 4-chlorophenol as a sole carbon source and shows a hydroxyquinol accumulation.
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
degradation
-
degradation of mixtures of phenolic compounds by Arthrobacter chlorophenolicus A6