Information on EC 1.13.11.37 - hydroxyquinol 1,2-dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
1.13.11.37
-
RECOMMENDED NAME
GeneOntology No.
hydroxyquinol 1,2-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
benzene-1,2,4-triol + O2 = maleylacetate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
2,4,5-trichlorophenoxyacetate degradation
-
4-aminophenol degradation
-
4-nitrophenol degradation II
-
Benzoate degradation
-
Chlorocyclohexane and chlorobenzene degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
resorcinol degradation
-
SYSTEMATIC NAME
IUBMB Comments
benzene-1,2,4-triol:oxygen 1,2-oxidoreductase (decyclizing)
An iron protein. Highly specific; catechol and pyrogallol are acted on at less than 1% of the rate at which hydroxyquinol is oxidized.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,2-HQD
Q5PXQ6
-
1,2-HQD
Pimelobacter simplex 3E
Q5PXQ6
-
-
hydroxyquinol 1,2-dioxygenase
-
-
hydroxyquinol 1,2-dioxygenase
-
-
hydroxyquinol 1,2-dioxygenase
-
-
-
hydroxyquinol-1,2-dioxygenase
-
-
hydroxyquinol-1,2-dioxygenase
Pseudomonas sp. HS-D38
-
-
-
NpcC
Rhodococcus opacus SAO101
-
-
-
oxygenase, hydroxyquinol 1,2-di-
-
-
-
-
oxygenase, hydroxyquinol di-
-
-
-
-
PdcC
-
gene name
PdcC
-
gene name
-
PnpC
-
gene name
PnpC
Pseudomonas sp. HS-D38
-
gene name
-
hydroxyquinon dioxygenase
-
-
-
-
additional information
Q3BEM2
cphA-1 encode hydroxyquinol 1,2-dioxygenases. The amino acid sequence of CphA-2 is 78.4% identical to that of CphA-1.
additional information
Q3BEN0
cphA-2 encodes hydroxyquinol 1,2-dioxygenases. The amino acid sequence of CphA-2 is 78.4% identical to that of CphA-1.
CAS REGISTRY NUMBER
COMMENTARY
91847-14-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain A6, DSM12829T
-
-
Manually annotated by BRENDA team
BA-5-17, grown in benzamide medium
SwissProt
Manually annotated by BRENDA team
Azotobacter sp.
strain GP1, DSM 6428, ATCC 49806, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
Azotobacter sp. GP1
strain GP1, DSM 6428, ATCC 49806, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
AC1100, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
L. ex Fr., 69346, grown with cellobiose
-
-
Manually annotated by BRENDA team
Drechslera oryzae
-
-
-
Manually annotated by BRENDA team
Fomes pinicola
Sw. ex Fr., Karst. B.H., grown with cellobiose
-
-
Manually annotated by BRENDA team
f. sp. vasinfectum
-
-
Manually annotated by BRENDA team
Lenzites trabea
Pers. ex Fr., Fr. Karst. A-576, grown with cellobiose
-
-
Manually annotated by BRENDA team
Pimelobacter simplex 3E
3E
SwissProt
Manually annotated by BRENDA team
Pimelobacter simplex 3E
strain 3E
-
-
Manually annotated by BRENDA team
Polyporus dichrous
Fr., grown with cellulose
-
-
Manually annotated by BRENDA team
Poria ambigua
Bres. BB3-1, grown with cellulose
-
-
Manually annotated by BRENDA team
gene pnpC
-
-
Manually annotated by BRENDA team
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA
-
-
Manually annotated by BRENDA team
strain PDS-7
-
-
Manually annotated by BRENDA team
isolated from methyl parathion-polluted activated sludge, gene pdcC encoded in gene cluster pdcEDGFCBA
-
-
Manually annotated by BRENDA team
Pseudomonas sp. HS-D38
gene pnpC
-
-
Manually annotated by BRENDA team
strain PDS-7
-
-
Manually annotated by BRENDA team
strain SAO101
-
-
Manually annotated by BRENDA team
Rhodococcus opacus SAO101
strain SAO101
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
grown in presence of vanillic acid
-
-
Manually annotated by BRENDA team
strain 303, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
Streptomyces rochei 303
strain 303, grown with 2,4,6-trichlorophenol
-
-
Manually annotated by BRENDA team
Jacq. ex Fr., Karst. A-360, grown with cellulose
-
-
Manually annotated by BRENDA team
grown in presence of L-tyrosine
-
-
Manually annotated by BRENDA team
grown with hydrobenzoate
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol inthe route via 4-nitrocatechol, overview
metabolism
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol in the route via 4-nitrocatechol, overview
metabolism
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol inthe route via 4-nitrocatechol, overview
-
metabolism
Pseudomonas sp. HS-D38
-
the enzyme is involved in degradation of 4-nitrophenol, the catabolism proceeds via two different intermediates, hydroquinone and 4-nitrocatechol, resulting in formation of maleylacetate. Hydroxyquinol 1,2-dioxygenase catalyzes the formation of maleylacetate from hydroxyquinol in the route via 4-nitrocatechol, overview
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
-
-
-
?
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
Azotobacter sp.
-
33.5% relative activity of that measured with hydroxyquinol
-
?
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
Streptomyces rochei 303
-
-
-
?
6-chlorohydroxyquinol + O2
2-chloromaleylacetate
show the reaction diagram
Azotobacter sp. GP1
-
33.5% relative activity of that measured with hydroxyquinol
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Azotobacter sp.
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Fomes pinicola, Lenzites trabea, Poria ambigua, Daedalea quercina, Polyporus dichrous
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Chaetomium cupreum, Drechslera oryzae
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Q9S1B4
6.8-fold activity of that for catechol
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Streptomyces rochei 303
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Pseudomonas sp. HS-D38
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Azotobacter sp. GP1
-
-
maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Burkholderia cepacia AC1100
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp.
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Fomes pinicola, Lenzites trabea, Poria ambigua, Daedalea quercina, Polyporus dichrous
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Chaetomium cupreum, Drechslera oryzae
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Q9S1B4
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Streptomyces rochei 303
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp. GP1
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Burkholderia cepacia AC1100
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Q5PXQ6
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Rhodococcus opacus SAO101
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Pimelobacter simplex 3E
Q5PXQ6
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
cis,cis-muconic acid
show the reaction diagram
-
-
-
-
?
catechol + O2
cis,cis-muconic acid
show the reaction diagram
Q9S1B4
intradiol ring cleavage
-
?
catechol + O2
2-hydroxymuconic 6-semialdehyde
show the reaction diagram
Q9S1B4
extradiol ring cleavage
-
?
hydroxyquinol + O2
?
show the reaction diagram
-
-
-
-
?
hydroxyquinol + O2
maleylacetate
show the reaction diagram
-
intradiol ringcleavage
-
-
?
additional information
?
-
-, Q3BEM2, Q3BEN0
CphA-1 exhibits no activity with 4-chlorocatechol, hydroquinone, or resorcinol
-
-
-
additional information
?
-
-, Q3BEM2, Q3BEN0
CphA-2 exhibits no activity with 4-chlorocatechol, hydroquinone, or resorcinol
-
-
-
additional information
?
-
-
Pseudomonas sp. PDS-7 is capable of utilizing p-nitrophenol as the sole carbon, nitogen and energy source, concentration up to 80 mg/l
-
-
-
additional information
?
-
-
when the cells are grown on a mixture of 4-chlorophenol, 4-nitrophenol and phenol, 4-chlorophenol degradation apparently is delayed until 4-nitrophenol is almost completely depleted. Phenol is degraded more slowly than the other compounds and not until 4-nitrophenol and 4-chlorophenol are depleted, despite this being the least toxic compound of the three
-
-
-
additional information
?
-
-
PnpG is not active against protocatechuate and gentisate
-
-
-
additional information
?
-
-
Pseudomonas sp. PDS-7 is capable of utilizing p-nitrophenol as the sole carbon, nitogen and energy source, concentration up to 80 mg/l
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp.
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Fomes pinicola, Lenzites trabea, Poria ambigua, Daedalea quercina, Polyporus dichrous
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Chaetomium cupreum, Drechslera oryzae
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Q9S1B4
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Q5PXQ6
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Streptomyces rochei 303
-
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis,cis-muconate
show the reaction diagram
Pimelobacter simplex 3E
Q5PXQ6
-
-
-
?
benzene-1,2,4-triol + O2
maleylacetate
show the reaction diagram
Pseudomonas sp. HS-D38
-
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Azotobacter sp. GP1
-
-
-
?
benzene-1,2,4-triol + O2
3-hydroxy-cis-cis-muconate
show the reaction diagram
Burkholderia cepacia AC1100
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
stimulates at 0.2 mM
Cu2+
-
stimulates at 0.2 mM
Fe2+
-
stimulates at 0.2 mM
Fe3+
-
catalytically essential
Fe3+
Q5PXQ6
required for activity
Fe3+
-
stimulates at 0.2 mM
Iron
Azotobacter sp.
-
0.18 mol per mol enzyme
Iron
Azotobacter sp.
-
1 mol of iron per mol enzyme
Iron
-
1 mol of iron per mol enzyme
Mn2+
-
stimulates at 0.2 mM
Zn2+
-
stimulates at 0.2 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,4-Dichlorophenol
Azotobacter sp.
-
mixed inhibition
2,4-Dichlorophenol
-
competitive inhibition
2,6-dichlorohydroquinone
Azotobacter sp.
-
competitive nhibition
2,6-dichlorohydroquinone
-
competitive inhibition
3,4,6-trichlorocatechol
Azotobacter sp., Streptomyces rochei
-
mixed inhibition
3,5-dichlorocatechol
Azotobacter sp.
-
competitive inhibition
3,5-dichlorocatechol
-
mixed inhibition
3-chlorocatechol
Azotobacter sp.
-
competitive inhibition
3-chlorocatechol
-
mixd inhibition
3-methyl-5-chlorocatechol
Azotobacter sp.
-
competitive inhibition
3-methyl-5-chlorocatechol
-
mixed inhibition
4,5-dichlorocatechol
Azotobacter sp.
-
competitive inhibition
4,5-dichlorocatechol
-
parially mixed competitive inhibition
4-Chlorocatechol
Azotobacter sp., Streptomyces rochei
-
competitive inhibition
catechol
Azotobacter sp.
-
mixed inhibition
EDTA
Azotobacter sp.
-
slight inhibition
monochlorohydroquinone
Azotobacter sp., Streptomyces rochei
-
competitive inhibition
pyrogallol
Azotobacter sp.
-
mixed inhibition
tetrachlorocatechol
Azotobacter sp., Streptomyces rochei
-
mixed inhibition
Tetrachlorohydroquinone
Azotobacter sp., Streptomyces rochei
-
competitive inhibition
Tiron
Azotobacter sp.
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Fe(NH4)2(SO4)2
Azotobacter sp.
-
0.00001-0.000001 mM
Fe(NH4)2(SO4)2
Azotobacter sp.
-
no activation by Fe2+ ions
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.06
-
6-chlorohydroxyquinol
-
-
0.0012
-
Benzene-1,2,4-triol
Q5PXQ6
-
3.5e-05
-
Hydroxyquinol
Sporotrichum pulverulentum
-
-
0.0057
-
Hydroxyquinol
-
-
0.007
-
Hydroxyquinol
-
-
0.02
-
Hydroxyquinol
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
72.3
-
6-chlorohydroxyquinol
-
-
29
-
Benzene-1,2,4-triol
Q5PXQ6
-
6.35
-
Hydroxyquinol
-
-
11.4
-
Hydroxyquinol
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01274
-
2,4-Dichlorophenol
Azotobacter sp.
-
-
0.01719
-
2,4-Dichlorophenol
-
-
0.00589
-
2,6-dichlorohydroquinone
-
-
0.01094
-
2,6-dichlorohydroquinone
Azotobacter sp.
-
-
0.00536
-
3,4,6-trichlorocatechol
-
-
0.0112
-
3,4,6-trichlorocatechol
Azotobacter sp.
-
-
0.0088
-
3,5-dichlorocatechol
Azotobacter sp.
-
-
0.0169
-
3,5-dichlorocatechol
-
-
0.00698
-
3-chlorocatechol
Azotobacter sp.
-
-
0.03077
-
3-chlorocatechol
-
-
0.02732
-
3-methyl-5-chlorocatechol
Azotobacter sp.
-
-
0.0373
-
3-methyl-5-chlorocatechol
-
-
0.00585
-
4,5-dichlorocatechol
-
-
0.0103
-
4,5-dichlorocatechol
Azotobacter sp.
-
-
0.0073
-
4-Chlorocatechol
Azotobacter sp.
-
-
0.0526
-
4-Chlorocatechol
-
-
0.00956
-
catechol
Azotobacter sp.
-
-
10
-
catechol
Q5PXQ6
approximate value
0.00083
-
monochlorohydroquinone
-
-
0.00107
-
monochlorohydroquinone
Azotobacter sp.
-
-
0.00069
-
pyrogallol
Azotobacter sp.
-
-
0.00128
-
tetrachlorocatechol
-
-
0.00349
-
tetrachlorocatechol
Azotobacter sp.
-
-
0.00226
-
Tetrachlorohydroquinone
-
-
0.01248
-
Tetrachlorohydroquinone
Azotobacter sp.
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.37
-
-
substrate hydroxyquinol
0.45
-
-
substrate catechol
7.06
-
Azotobacter sp.
-
reactivated with 0.01 mM Fe2+
9.3
-
-
purified recombinant His-tagged enzyme, pH 5.0, 45C
additional information
-
Azotobacter sp.
-
-
additional information
-
Q9S1B4
-
additional information
-
-, Q3BEM2, Q3BEN0
cell extracts from 4-chlorophenol-grown cells deplete hydoxyquinol from the medium with an activity of 96 microM/min/mg protein. No depletion of hydroxyquinol is measured in extracts of Succinate-grown cells.; cell extracts from 4-chlorophenol-grown cells deplete hydoxyquinol from the medium with an activity of 96 microM/min/mg protein. No depletion of hydroxyquinol is measured in extracts of Succinate-grown cells.; Cph-2 remove hydroxyquinol: CphA-2 = 3.0 microM/min/mg. CphA-2 show some activity with catechol: CphA-2 = 0.15 microM/min/mg.; CphA-1 remove hydroxyquinol: CphA-1 = 16 microM/min/mg. CphA-1 show some activity with catechol: CphA-1 = 0.67 microM/min/mg.
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
-
enzyme assay
6.4
-
Azotobacter sp.
-
in succinate buffer
6.5
-
Sporotrichum pulverulentum
-
enzyme assay
8.5
-
Q9S1B4
for catechol
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
Sporotrichum pulverulentum
-
enzyme assay
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Daedalea quercina, Fomes pinicola, Lenzites trabea, Polyporus dichrous, Poria ambigua, Trametes cinnabarina
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
34800
-
-
estimated from SDS-PAGE
40000
-
-, Q3BEM2, Q3BEN0
recombinant CphA-1
42000
-
-, Q3BEM2, Q3BEN0
recombinant CphA-2
58000
-
Azotobacter sp.
-
gel filtration
61000
-
-
gel filtration
68000
-
-
gel filtration
79000
-
Q9S1B4
gel filtration
93000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Q9S1B4
? * 36000, SDS-PAGE
?
-
x * 33200, deduced from nucleotide sequence
?
-
x * 33000, about, sequence calculation
?
Pseudomonas sp. HS-D38
-
x * 33000, about, sequence calculation
-
?
Rhodococcus opacus SAO101
-
x * 33200, deduced from nucleotide sequence
-
dimer
-
2 * 36000, SDS-PAGE
dimer
Azotobacter sp.
-
2 * 34250, SDS-PAGE
dimer
Azotobacter sp. GP1
-
2 * 34250, SDS-PAGE
-
dimer
Burkholderia cepacia AC1100
-
2 * 36000, SDS-PAGE
-
heterodimer
-
alpha 1 * 39600 + beta 1 * 38200, SDS-PAGE
homodimer
-
2 * 31000, SDS-PAGE
homodimer
-
two identical subunits in a alpha2-type quaternary strucure
homodimer
Pimelobacter simplex 3E
-
two identical subunits in a alpha2-type quaternary strucure
-
homodimer
Streptomyces rochei 303
-
2 * 31000, SDS-PAGE
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sitting drop vapor difffusion from a solution containing 2 M ammonium sulfate, 4% polyethylene glycol 400, 100 mM Hepes, pH 7.5, drops consist of 0.004 ml of 20 mg/ml protein solution and 0.006 ml reservoir solution equilibrated against 0.05 ml of reservoir solution, crystals diffract to 1.75 A
Q5PXQ6
unit-cell dimensions: a = 81.15, b = 86.79, c = 114.93
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
Q9S1B4
activity not influenced after incubation for 10 min
55
-
Q9S1B4
60% activity loss after 10 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, no loss of activity after several weeks, at room temperature no considerable decrease after 1 day
Azotobacter sp.
-
4C, stable for 2 to 3 weeks in elution buffer, can not be stored at 4C or frozen at -20C
-
4C, reasonably stable for several weeks in the presence of isopropanol
-
4C, loss of most of the activity after 1 week
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Azotobacter sp.
-
recombinant 1,2-HQD
Q5PXQ6
Ni-NTA column chromatography
-
recombinant His-tagged enzyme 37.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cphA-1 from Arthrobacter chloropenolicus A6 is cloned into the vectro pCRT7/CT-TOPO, the construct is transformed into CL21(DE3)/pLysS cells.; cphA-2 from Arthrobacter chloropenolicus A6 is cloned into the vectro pCRT7/CT-TOPO, the construct is transformed into CL21(DE3)/pLysS cells.
-, Q3BEM2, Q3BEN0
expressed in Escherichia coli
Q9S1B4
expressed in Escherichia coli
-
expression in Escherichia coli
Q5PXQ6
expressed in Escherichia coli BL21 cells
-
expression in Escherichia coli BL21
-
gene pnpC, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-, Q3BEM2, Q3BEN0
Arthrobacter chlorophenolicus A6 is transformed with plasmid pKGT452Cbeta, which contain an Arthrobacter-derived, randomly inserting transposon conferring chloramphenicol resistance. The transposon mutagenese to disrupt the cphA-1 gene generates mutant strain Arthrobacter chlorophenolicus T99. The T99 mutant is not able to grow on 4-chlorophenol as a sole carbon source and shows a hydroxyquinol accumulation.
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
degradation
-
degradation of mixtures of phenolic compounds by Arthrobacter chlorophenolicus A6