Information on EC 1.13.11.30 - stizolobinate synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
1.13.11.30
-
RECOMMENDED NAME
GeneOntology No.
stizolobinate synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-dopa + O2 = 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
lincomycin biosynthesis
-
Tyrosine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxy-L-phenylalanine:oxygen 2,3-oxidoreductase (recyclizing)
The intermediate product undergoes ring closure and oxidation, with NAD(P)+ as acceptor, to stizolobinic acid. The enzyme requires Zn2+.
CAS REGISTRY NUMBER
COMMENTARY
65979-38-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Stizolobium hassjoo
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-L-phenylalanine + O2
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
undergoes ring closure and oxidation to stizolobinate with NAD(P)+ as acceptor
?
3,4-dihydroxy-L-phenylalanine + O2
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
undergoes ring closure and oxidation to stizolobinate with NAD(P)+ as acceptor
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-L-phenylalanine + O2
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
show the reaction diagram
Stizolobium hassjoo
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NAD+
Stizolobium hassjoo
-
required for reaction, less efficient than NADP+
NAD+
Stizolobium hassjoo
-
-
NADP+
Stizolobium hassjoo
-
required for reaction
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Zn2+
Stizolobium hassjoo
-
activation
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
8-Hydroxyquinoline-5-sulfonate
Stizolobium hassjoo
-
90% inhibition at 0.1 mM
Al3+
Stizolobium hassjoo
-
95% inhibition at 0.1 mM
alpha,alpha'-dipyridyl
Stizolobium hassjoo
-
70% inhibition at 0.1 mM
Co2+
Stizolobium hassjoo
-
85% inhibition at 0.1 mM
Cu2+
Stizolobium hassjoo
-
85% inhibition at 0.1 mM
Fe2+
Stizolobium hassjoo
-
75% inhibition at 0.1 mM
-
Fe3+
Stizolobium hassjoo
-
95% inhibition at 0.1 mM
-
glutathione
Stizolobium hassjoo
-
70% inhibition at 0.1 mM
iodoacetate
Stizolobium hassjoo
-
55% inhibition at 0.1 mM
KCN
Stizolobium hassjoo
-
85% inhibition at 0.1 mM
Mg2+
Stizolobium hassjoo
-
65% inhibition at 0.1 mM
Mn2+
Stizolobium hassjoo
-
95% inhibition at 0.1 mM
Mo6+
Stizolobium hassjoo
-
80% inhibition at 0.1 mM
N-ethylmaleimide
Stizolobium hassjoo
-
35% inhibition at 0.1 mM
Neocuproine
Stizolobium hassjoo
-
50% inhibition at 0.1 mM
o-phenanthroline
Stizolobium hassjoo
-
90% inhibition at 0.1 mM
p-chloromercuribenzoate
Stizolobium hassjoo
-
70% inhibition at 0.1 mM
Salicylaldoxime
Stizolobium hassjoo
-
65% inhibition at 0.1 mM
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.67
-
3,4-dihydroxyphenylalanine
Stizolobium hassjoo
-
-
0.00026
-
NADP+
Stizolobium hassjoo
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.031
-
Stizolobium hassjoo
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.6
-
Stizolobium hassjoo
-
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Stizolobium hassjoo
-
-
Manually annotated by BRENDA team
Stizolobium hassjoo
-
etiolated
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45000
-
Stizolobium hassjoo
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DTT stabilizes
Stizolobium hassjoo
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, 0.1 mM DTT, nitrogen atmosphere, stable for 3 months
Stizolobium hassjoo
-
30°C, purified enzyme, half life of 3-5 h
Stizolobium hassjoo
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Stizolobium hassjoo
-